Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,442,663	G→A	L61L (CTG→TTG)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,442,663	0	G	A	100.0%	230.8 / NA	67	L61L (CTG→TTG)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/31);  total (36/31)

GCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAG  >  NC_000913/1442533‑1442798                                                                                                                                   |                                                                                                                                        gCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGggcg                                                                                                                                >  2:1418818/1‑140 (MQ=255) gCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGATCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGggcg                                                                                                                                >  2:413767/1‑140 (MQ=255)    tctGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCt                                                                                                                             >  1:1119564/1‑140 (MQ=255)      tGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGc                                                                                                                           >  2:1167020/1‑140 (MQ=255)           cATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCa                                                                                                                      <  2:1119564/140‑1 (MQ=255)            aTAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCAt                                                                                                                     >  1:1067416/1‑140 (MQ=255)            aTAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCAt                                                                                                                     >  2:614217/1‑140 (MQ=255)             tAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATc                                                                                                                    >  2:406177/1‑140 (MQ=255)                gCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGtt                                                                                                                 <  2:1230053/140‑1 (MQ=255)                gCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGtt                                                                                                                 >  2:1122449/1‑140 (MQ=255)                gCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGtt                                                                                                                 <  2:884737/140‑1 (MQ=255)                gCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGtt                                                                                                                 >  1:195442/1‑140 (MQ=255)                  tGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTa                                                                                                               <  1:1167020/140‑1 (MQ=255)                   ggAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTa                                                                                                               >  2:498312/1‑139 (MQ=255)                   ggAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTa                                                                                                               <  1:498312/139‑1 (MQ=255)                   ggAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTAc                                                                                                              >  2:1244841/1‑140 (MQ=255)                   ggAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTAc                                                                                                              >  2:53563/1‑140 (MQ=255)                    gAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACg                                                                                                             <  1:26225/140‑1 (MQ=255)                    gAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACg                                                                                                             <  1:614217/140‑1 (MQ=255)                    gAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACg                                                                                                             >  2:1172580/1‑140 (MQ=255)                     aaCACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGa                                                                                                            >  1:221520/1‑140 (MQ=255)                         cGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAat                                                                                                        <  1:1172580/140‑1 (MQ=255)                           gACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGtt                                                                                                                 <  2:125745/129‑1 (MQ=255)                           gACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGtt                                                                                                                 >  1:125745/1‑129 (MQ=255)                            actactTTCAGCCCCAGTTCTTTTGCCGCGTCAAGATCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAataca                                                                                                     <  1:413767/140‑1 (MQ=255)                                 tttCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCg                                                                                                <  1:1244841/140‑1 (MQ=255)                                 tttCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCg                                                                                                <  1:53563/140‑1 (MQ=255)                                 tttCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCg                                                                                                <  2:221520/140‑1 (MQ=255)                                     aGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGaaa                                                                                                          <  2:913356/126‑1 (MQ=255)                                     aGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGaaa                                                                                                          >  1:913356/1‑126 (MQ=255)                                      gCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCg                                                                                           >  1:740223/1‑140 (MQ=255)                                                        cgTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTa                                                                         <  2:415954/140‑1 (MQ=255)                                                                    cGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTcc                                                             <  2:1050722/140‑1 (MQ=255)                                                                      ttATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGt                                                           <  1:307782/140‑1 (MQ=255)                                                                         ttGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTcag                                                        >  1:410548/1‑140 (MQ=255)                                                                          tGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTcagc                                                       >  1:809148/1‑140 (MQ=255)                                                                               ccGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGaaa                                                  >  1:350765/1‑140 (MQ=255)                                                                                cGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAg                                                 <  1:1102265/140‑1 (MQ=255)                                                                                 ggCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGt                                                >  2:419541/1‑140 (MQ=255)                                                                                   cacaGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGACAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCa                                              >  2:579486/1‑140 (MQ=255)                                                                                      aGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCaaaa                                           >  2:1406560/1‑140 (MQ=255)                                                                                             ggCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCa                                    >  1:263415/1‑140 (MQ=255)                                                                                             ggCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCa                                    >  1:665917/1‑140 (MQ=255)                                                                                                gATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCt                                 >  1:835282/1‑140 (MQ=255)                                                                                                        tAACGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCa                         <  2:263415/140‑1 (MQ=255)                                                                                                          aCGCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACG‑‑AATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAgg                     <  1:406177/140‑1 (MQ=255)                                                                                                            gCCGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAgg                     >  1:357423/1‑140 (MQ=255)                                                                                                             ccGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGa                    <  2:665917/140‑1 (MQ=255)                                                                                                              cGTGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCTAAAGGAc                   >  2:225761/1‑140 (MQ=255)                                                                                                                tGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAgg                     >  2:801838/1‑136 (MQ=255)                                                                                                                tGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAgg                     <  1:801838/136‑1 (MQ=255)                                                                                                                tGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTc                 >  2:983206/1‑140 (MQ=255)                                                                                                                tGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTc                 >  1:1228894/1‑140 (MQ=255)                                                                                                                tGCTTTTTCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTc                 >  2:483778/1‑140 (MQ=255)                                                                                                                      ttCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCAc           <  2:195442/140‑1 (MQ=255)                                                                                                                      ttCAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCAc           <  2:740223/140‑1 (MQ=255)                                                                                                                        cAGCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCt         <  2:603673/140‑1 (MQ=255)                                                                                                                          gCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCa            <  1:597100/135‑1 (MQ=255)                                                                                                                          gCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCa            >  2:597100/1‑135 (MQ=255)                                                                                                                          gCTCTTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGt       >  1:811318/1‑140 (MQ=255)                                                                                                                           ctctTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGtt      <  2:357423/140‑1 (MQ=255)                                                                                                                            tctTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGtt      >  2:869661/1‑139 (MQ=255)                                                                                                                            tctTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGtt      <  1:869661/139‑1 (MQ=255)                                                                                                                             ctTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCt         <  2:208581/135‑1 (MQ=255)                                                                                                                             ctTCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCt         >  1:208581/1‑135 (MQ=255)                                                                                                                               tCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAg  <  2:15929/140‑1 (MQ=255)                                                                                                                               tCCAACACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAg  <  2:1067416/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAG  >  NC_000913/1442533‑1442798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑