Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,591,989	T→C	N63S (AAC→AGC)	hipA ←	serine/threonine kinase HipA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,591,989	0	T	C	100.0%	307.7 / NA	89	N63S (AAC→AGC)	hipA	serine/threonine kinase HipA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/48);  total (41/48)

TCGTCTTCGGGTATTAACGTCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGTTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAA  >  NC_000913/1591854‑1592122                                                                                                                                        |                                                                                                                                      tCGTCTTCGGGTATTAACGTCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATc                                                                                                                                   >  2:1013225/1‑140 (MQ=255) tCGTCTTCGGGTATTAACGTCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATc                                                                                                                                   >  1:652316/1‑140 (MQ=255)     cTTCGGGTATTAACGTCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCgaag                                                                                                                               <  2:397809/140‑1 (MQ=255)     cTTCGGGTATTAACGTCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCgaag                                                                                                                               <  1:225821/140‑1 (MQ=255)           gTATTAACGTCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTa                                                                                                                         >  2:955998/1‑140 (MQ=255)                aaCGTCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATAc                                                                                                                    <  2:1284519/140‑1 (MQ=255)                aaCGTCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATAc                                                                                                                    <  2:74915/140‑1 (MQ=255)                    tCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATATGGCa                                                                                                                <  2:652316/140‑1 (MQ=255)                     cACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATAc                                                                                                                    >  1:1387322/1‑135 (MQ=255)                     cACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATAc                                                                                                                    <  2:1387322/135‑1 (MQ=255)                     cACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCAt                                                                                                               >  1:856206/1‑140 (MQ=255)                     cACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCAt                                                                                                               >  2:366716/1‑140 (MQ=255)                     cACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCAt                                                                                                               <  2:1088092/140‑1 (MQ=255)                     cACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCAt                                                                                                               >  2:1327818/1‑140 (MQ=255)                      aCGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTa                                                                                                                         <  2:396743/129‑1 (MQ=255)                      aCGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTa                                                                                                                         <  2:1000777/129‑1 (MQ=255)                      aCGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTa                                                                                                                         >  1:1000777/1‑129 (MQ=255)                      aCGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTa                                                                                                                         >  1:396743/1‑129 (MQ=255)                      aCGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATc                                                                                                              >  1:1200622/1‑140 (MQ=255)                      aCGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATc                                                                                                              >  1:1147798/1‑140 (MQ=255)                       cGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCa                                                                                                             >  1:905923/1‑140 (MQ=255)                        ggCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCag                                                                                                            >  2:689625/1‑140 (MQ=255)                          catcAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCagag                                                                                                          <  1:458148/137‑1 (MQ=255)                            ccAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCAt                                                                                                               >  1:328274/1‑133 (MQ=255)                            ccAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCAt                                                                                                               <  2:328274/133‑1 (MQ=255)                            ccAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGt                                                                                                        >  1:1304175/1‑140 (MQ=255)                            ccAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGt                                                                                                        <  1:955998/140‑1 (MQ=255)                             cAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGAATCAGAGGTg                                                                                                       >  2:218270/1‑140 (MQ=255)                               aCGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGat                                                                                                     >  1:1135204/1‑140 (MQ=255)                               aCGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGat                                                                                                     >  1:1290948/1‑140 (MQ=255)                                 gCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGatat                                                                                                   <  2:905923/140‑1 (MQ=255)                                 gCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGatat                                                                                                   <  2:1147798/140‑1 (MQ=255)                                 gCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGatat                                                                                                   <  2:1135204/140‑1 (MQ=255)                                 gCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGatat                                                                                                   <  1:1430255/140‑1 (MQ=255)                                  cTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATAtt                                                                                                  >  1:59427/1‑140 (MQ=255)                                     tctcGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTccc                                                                                               <  2:856206/140‑1 (MQ=255)                                     tctcGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGAATCAGAGGTGATATTccc                                                                                               <  1:218270/140‑1 (MQ=255)                                      ctcGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTcccc                                                                                              <  1:1213932/140‑1 (MQ=255)                                      ctcGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTcccc                                                                                              <  1:689625/140‑1 (MQ=255)                                       tcGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTccccc                                                                                             <  1:1401026/140‑1 (MQ=255)                                          cccTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCctct                                                                                          >  1:894602/1‑140 (MQ=255)                                                 tCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATgg                                                                                   >  2:1453115/1‑140 (MQ=255)                                                     aCAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGc                                                                               >  2:1070332/1‑140 (MQ=255)                                                      cAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCg                                                                              <  2:894602/140‑1 (MQ=255)                                                         tAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGaaa                                                                           >  2:53348/1‑140 (MQ=255)                                                         tAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGaaa                                                                           >  1:154817/1‑140 (MQ=255)                                                            aTCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTg                                                                        >  2:363511/1‑140 (MQ=255)                                                               aaaCGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACa                                                                     <  1:1195633/140‑1 (MQ=255)                                                               aaaCGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACa                                                                     <  2:1200622/140‑1 (MQ=255)                                                                 aCGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACaa                                                                    <  2:1184447/139‑1 (MQ=255)                                                                 aCGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACaa                                                                    >  1:1184447/1‑139 (MQ=255)                                                                 aCGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAAc                                                                   >  1:1393722/1‑140 (MQ=255)                                                                   ggTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACgg                                                                 <  1:1453115/140‑1 (MQ=255)                                                                    gTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGt                                                                <  1:1327818/140‑1 (MQ=255)                                                                     ttGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTc                                                               <  1:366716/140‑1 (MQ=255)                                                                     ttGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTc                                                               <  2:59427/140‑1 (MQ=255)                                                                     ttGACTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTc                                                               >  1:1276726/1‑140 (MQ=255)                                                                             aTTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACa                                                                     >  1:137945/1‑126 (MQ=255)                                                                             aTTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACa                                                                     <  2:137945/126‑1 (MQ=255)                                                                               ttGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACg                                                     <  1:1036562/140‑1 (MQ=255)                                                                               ttGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACg                                                     <  1:376118/140‑1 (MQ=255)                                                                               ttGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACg                                                     <  2:1304175/140‑1 (MQ=255)                                                                                  gCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCt                                                  >  2:1090268/1‑140 (MQ=255)                                                                                             tttAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACt                                       <  2:1393722/140‑1 (MQ=255)                                                                                             tttAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACt                                       <  2:1463441/140‑1 (MQ=255)                                                                                             tttAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACt                                       <  2:707888/140‑1 (MQ=255)                                                                                                    aTCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                <  1:53348/140‑1 (MQ=255)                                                                                                    aTCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                <  2:154817/140‑1 (MQ=255)                                                                                                    aTCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                <  2:252873/140‑1 (MQ=255)                                                                                                    aTCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                <  2:1290948/140‑1 (MQ=255)                                                                                                    aTCCGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                <  1:401980/140‑1 (MQ=255)                                                                                                      ccGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                >  1:788559/1‑138 (MQ=255)                                                                                                      ccGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                >  2:452979/1‑138 (MQ=255)                                                                                                      ccGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                <  1:452979/138‑1 (MQ=255)                                                                                                      ccGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                <  2:788559/138‑1 (MQ=255)                                                                                                       cGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                >  2:693605/1‑137 (MQ=255)                                                                                                       cGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                >  2:1018997/1‑137 (MQ=255)                                                                                                       cGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                <  1:693605/137‑1 (MQ=255)                                                                                                       cGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGc                                <  1:1018997/137‑1 (MQ=255)                                                                                                       cGGTCACGTACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATa                             >  2:1124650/1‑140 (MQ=255)                                                                                                               tACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAgg                     >  2:317198/1‑140 (MQ=255)                                                                                                               tACAATCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAgg                     >  1:1111772/1‑140 (MQ=255)                                                                                                                    tCGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGCGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTgc                >  2:1226612/1‑140 (MQ=255)                                                                                                                     cGGGCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTgcg               <  2:705569/140‑1 (MQ=255)                                                                                                                      gggCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTgcgc              >  1:601865/1‑140 (MQ=255)                                                                                                                        gCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTgcgcgc            >  1:926191/1‑140 (MQ=255)                                                                                                                        gCTATCGGGTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTgcgcgc            >  2:842538/1‑140 (MQ=255)                                                                                                                                gTAACAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCt    <  2:1276726/140‑1 (MQ=255)                                                                                                                                  aaCAGGCTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTaa  <  2:926191/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      TCGTCTTCGGGTATTAACGTCACGGCACCAACGCTGTCTCGCCCTATTTCTGACAATAAATCAAACGGTTGTCTGGATTTGGCATGATAACGTTTAACGATCCGGTCACGTACAATCGGGCTATCGGGTAACAGGTTATCGAAGAAGTTAAATACGGCATCAGAGGTGATATTCCCCCTCTGCAATGGCAGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAA  >  NC_000913/1591854‑1592122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑