Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,661,994	G→A	G480D (GGC→GAC)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,661,994	0	G	A	100.0%	352.5 / NA	99	G480D (GGC→GAC)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (54/45);  total (54/45)

CTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACAGAGCAAGAA  >  NC_000913/1661857‑1662130                                                                                                                                          |                                                                                                                                         cTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACg                                                                                                                                        >  1:882690/1‑140 (MQ=255)     aCATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACat                                                                                                                                    <  1:512831/140‑1 (MQ=255)         ggACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACatcaat                                                                                                                                <  2:826245/140‑1 (MQ=255)           aCAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACatcaatca                                                                                                                              <  2:1235760/140‑1 (MQ=255)           aCAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACatcaatca                                                                                                                              <  2:427133/140‑1 (MQ=255)           aCAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACatcaatca                                                                                                                              <  1:42852/140‑1 (MQ=255)              gATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGCTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCacac                                                                                                                           >  1:505274/1‑140 (MQ=255)               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACAcc                                                                                                                          >  1:111238/1‑140 (MQ=255)               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACAcc                                                                                                                          >  2:1196947/1‑140 (MQ=255)               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACAcc                                                                                                                          >  2:535737/1‑140 (MQ=255)               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACAcc                                                                                                                          >  2:467554/1‑140 (MQ=255)               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACAcc                                                                                                                          >  1:279748/1‑140 (MQ=255)                    aTCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGa                                                                                                                     >  1:1164501/1‑140 (MQ=255)                    aTCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGa                                                                                                                     >  2:393436/1‑140 (MQ=255)                     tCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAg                                                                                                                    >  1:883494/1‑140 (MQ=255)                     tCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAg                                                                                                                    >  2:143916/1‑140 (MQ=255)                      cGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAgg                                                                                                                   >  2:966919/1‑140 (MQ=255)                      cGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAgg                                                                                                                   <  1:393436/140‑1 (MQ=255)                       gACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGt                                                                                                                  <  1:1196947/140‑1 (MQ=255)                       gACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTT‑‑TGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGc                                                                                                                <  1:419241/140‑1 (MQ=255)                         ccATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGc                                                                                                                >  2:173821/1‑140 (MQ=255)                           aTGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCtt                                                                                                              <  2:279748/140‑1 (MQ=255)                            tGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTc                                                                                                             >  2:844129/1‑140 (MQ=255)                             ggTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCa                                                                                                                        >  1:585553/1‑128 (MQ=255)                             ggTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCa                                                                                                                        <  2:585553/128‑1 (MQ=255)                               tACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGc                                                                                                                >  1:1280746/1‑134 (MQ=255)                               tACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGc                                                                                                                <  2:1280746/134‑1 (MQ=255)                                   gAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGa                                                                                                      >  2:258776/1‑140 (MQ=255)                                       tGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGc                                                                                                  >  1:823022/1‑140 (MQ=255)                                       tGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGc                                                                                                  >  1:88435/1‑140 (MQ=255)                                       tGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGc                                                                                                  <  2:111238/140‑1 (MQ=255)                                       tGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGc                                                                                                  >  2:607768/1‑140 (MQ=255)                                        gACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCa                                                                                                 >  2:903927/1‑140 (MQ=255)                                        gACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCa                                                                                                 <  1:831569/140‑1 (MQ=255)                                        gACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCa                                                                                                 >  1:1383296/1‑140 (MQ=255)                                         aCCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCaa                                                                                                >  2:1408546/1‑140 (MQ=255)                                               aCCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCga                                                                                          <  2:1022596/140‑1 (MQ=255)                                                  cGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgat                                                                                       <  2:88435/140‑1 (MQ=255)                                                          tgtTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGAttgttg                                                                               >  1:517244/1‑140 (MQ=255)                                                            ttCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgat                                                                                       <  2:1029425/130‑1 (MQ=255)                                                            ttCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgat                                                                                       >  1:1029425/1‑130 (MQ=255)                                                            ttCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGc                                                                             >  1:1345126/1‑140 (MQ=255)                                                            ttCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGc                                                                             >  2:615717/1‑140 (MQ=255)                                                             tCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCa                                                                            <  1:1034818/140‑1 (MQ=255)                                                             tCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCa                                                                            >  1:1042692/1‑140 (MQ=255)                                                              cGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGTATGACAGCAAGTGCGAGATGATTGTTGGCAt                                                                           >  1:283415/1‑140 (MQ=255)                                                              cGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCAt                                                                           >  1:89712/1‑140 (MQ=255)                                                                  gaaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGAc                                                                       <  1:615717/140‑1 (MQ=255)                                                                  gaaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGAc                                                                       <  1:966919/140‑1 (MQ=255)                                                                   aaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGAcc                                                                      >  2:759563/1‑140 (MQ=255)                                                                     aagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCAc                                                                    <  1:607768/140‑1 (MQ=255)                                                                      agaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGAttgt                                                                                 <  2:57518/126‑1 (MQ=255)                                                                      agaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGAttgt                                                                                 >  1:57518/1‑126 (MQ=255)                                                                             cTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGAc                                                            >  2:883325/1‑140 (MQ=255)                                                                                gATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGc                                                         <  1:467554/140‑1 (MQ=255)                                                                                gATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGc                                                         <  1:258776/140‑1 (MQ=255)                                                                                gATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGc                                                         <  1:1408546/140‑1 (MQ=255)                                                                                     ccccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCgg                                                    >  1:1148770/1‑140 (MQ=255)                                                                                      cccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGTATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGc                                                   <  2:283415/140‑1 (MQ=255)                                                                                       ccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCt                                                  <  1:759563/140‑1 (MQ=255)                                                                                       ccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCt                                                  <  2:1148770/140‑1 (MQ=255)                                                                                       ccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCt                                                  <  2:89712/140‑1 (MQ=255)                                                                                          tCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATAACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAg                                               >  2:1019758/1‑140 (MQ=255)                                                                                               tttttATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTg                                          <  2:202044/140‑1 (MQ=255)                                                                                               tttttATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTg                                          <  1:173821/140‑1 (MQ=255)                                                                                                 tttATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCg                                        <  1:1019758/140‑1 (MQ=255)                                                                                                 tttATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCg                                        >  2:592977/1‑140 (MQ=255)                                                                                                   tATGGTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGat                                      <  2:605999/140‑1 (MQ=255)                                                                                                      ggTGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATc                                   >  2:1386423/1‑140 (MQ=255)                                                                                                        tGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCt                                 >  2:158172/1‑140 (MQ=255)                                                                                                        tGCTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCt                                 >  2:600429/1‑140 (MQ=255)                                                                                                          cTACGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATCACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGt                               >  1:564953/1‑140 (MQ=255)                                                                                                             cGCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGc                            <  2:823022/140‑1 (MQ=255)                                                                                                              gCCAGTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGcc                           <  2:1389501/140‑1 (MQ=255)                                                                                                                  gTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGAc                       >  2:680425/1‑140 (MQ=255)                                                                                                                  gTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGAc                       >  1:1110023/1‑140 (MQ=255)                                                                                                                  gTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGAc                       >  1:683403/1‑140 (MQ=255)                                                                                                                  gTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGAc                       <  2:1345126/140‑1 (MQ=255)                                                                                                                  gTAACACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGAc                       >  1:249593/1‑140 (MQ=255)                                                                                                                    aaCACATTGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCt                     <  2:1110023/140‑1 (MQ=255)                                                                                                                          ttGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGcc                           >  2:9426/1‑128 (MQ=255)                                                                                                                          ttGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGcc                           <  1:9426/128‑1 (MQ=255)                                                                                                                          ttGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGcc               >  1:488105/1‑140 (MQ=255)                                                                                                                           tGATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCa              <  1:903927/140‑1 (MQ=255)                                                                                                                            gATCAATCAACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCaa             >  1:963178/1‑140 (MQ=255)                                                                                                                             atcaatcaACATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAAc            >  1:558064/1‑140 (MQ=255)                                                                                                                               caatcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACag          <  2:505274/140‑1 (MQ=255)                                                                                                                               caatcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACag          >  1:198202/1‑140 (MQ=255)                                                                                                                               caatcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACag          <  1:118445/140‑1 (MQ=255)                                                                                                                               caatcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACag          <  2:488105/140‑1 (MQ=255)                                                                                                                                 atcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACagag        >  2:1263015/1‑140 (MQ=255)                                                                                                                                 atcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACagag        >  1:531138/1‑140 (MQ=255)                                                                                                                                  tcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACag          >  2:1301013/1‑137 (MQ=255)                                                                                                                                  tcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACag          <  1:1301013/137‑1 (MQ=255)                                                                                                                                  tcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACAGAGc       <  2:249593/140‑1 (MQ=255)                                                                                                                                  tcaaCATGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACAGAGc       >  1:1208791/1‑140 (MQ=255)                                                                                                                                       aTGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACaga         <  1:1121736/133‑1 (MQ=255)                                                                                                                                       aTGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACAGAGCaagaa  >  2:520673/1‑140 (MQ=255)                                                                                                                                       aTGACGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACAGAGCaaga   <  1:241650/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTTGCCCGACCTGATGCCAACAGAGCAAGAA  >  NC_000913/1661857‑1662130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑