Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,776,263 T→C V226A (GTG→GCG)  ydiF → putative acetate‑CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,776,2630TC100.0% 98.2 / NA 30V226A (GTG→GCG) ydiFputative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/29);  total (1/29)

CCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGT  >  NC_000913/1776127‑1776399
                                                                                                                                        |                                                                                                                                        
ccAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCa                                                                                                                                       <  1:523180/140‑1 (MQ=255)
       ttGCCTTCATTCGCGCTTCCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATg                                                                                                                                <  1:940620/140‑1 (MQ=255)
       ttGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGGATTTTGATGTAGCAGGCGCATaaaaac                                                                                                                                >  2:523180/1‑138 (MQ=255)
          ccTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGtt                                                                                                                             <  1:992531/140‑1 (MQ=255)
          ccTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGtt                                                                                                                             <  1:1371766/140‑1 (MQ=255)
             tCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAg                                                                                                                          <  2:460735/140‑1 (MQ=255)
               aTTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGaa                                                                                                                        >  1:1200563/1‑140 (MQ=255)
               aTTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGAATTGTGATGATGTAGGGGCCGAAAAAGGATGAGaa                                                                                                                        >  2:1371766/1‑140 (MQ=255)
                ttCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAAAGGGTaaaaaa                                                                                                                       >  2:1270998/1‑140 (MQ=255)
                         ccaccTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTg                                                                                                              <  1:100023/140‑1 (MQ=255)
                         ccaccTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTg                                                                                                              <  2:413746/140‑1 (MQ=255)
                         ccaccTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTg                                                                                                              <  1:625315/140‑1 (MQ=255)
                         ccaccTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTg                                                                                                              <  1:158931/140‑1 (MQ=255)
                                   cAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGATGATGCAGGCGCCGAAAAAGGGTAAGAAAGCCACGCTGCATCCTAAAt                                                                                                    >  1:511036/1‑140 (MQ=255)
                                    aGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGGTGATGCAGGCGCAGAAAAAGGTTAAGAAAGACACCCCGCATCCTAAAtt                                                                                                   >  2:381083/1‑139 (MQ=255)
                                    aGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                                                                                   <  1:37693/140‑1 (MQ=255)
                                    aGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                                                                                   <  2:1200563/140‑1 (MQ=255)
                                             tACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTAt                                                                                          <  1:1270998/140‑1 (MQ=255)
                                              aCGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTAtt                                                                                         <  1:381083/140‑1 (MQ=255)
                                                gCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTcc                                                                                       <  2:914804/140‑1 (MQ=255)
                                                          aaGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAAAGGTTAAGAAAGCCACGCTGCATCCTAAAACTGTCCGTATTCCGGGTTAtttt                                                                             >  2:1189237/1‑137 (MQ=255)
                                                              tGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCtggt                                                                           <  2:397288/138‑1 (MQ=255)
                                                              tGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAAAGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCtggt                                                                           >  1:397288/1‑138 (MQ=255)
                                                                 ggTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGata                                                                      <  1:972900/140‑1 (MQ=255)
                                                                      tGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATtgtg                                                                 <  1:1168880/140‑1 (MQ=255)
                                                                          tctcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGGGCCGAAAAAGGGTTAGAAAACCACGCTGCATCCTAAATCTTTCCGTATTTCCGGTTTTCTGGGGGGGATTGtggtgg                                                             >  2:573718/1‑140 (MQ=255)
                                                                           ctcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTggtggt                                                            <  1:247842/140‑1 (MQ=255)
                                                                           ctcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTggtggt                                                            <  2:883421/140‑1 (MQ=255)
                                                                           ctcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTggtggt                                                            <  1:1189237/140‑1 (MQ=255)
                                                                            tcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGATGATGCAGGGGCAGAAAAAGGTTAAAAAAACCACGCCGCATCCTTAAACTGTTCGTATTTCGGGGTTTTTTGGGGGTATTGTtgtggtg                                                           >  2:1072384/1‑139 (MQ=255)
                                                                             cGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTTGTGATGGAGGCGCAGAAAAAGGGTTAAAAAACCACGCTGCATCCTAAATCTGTCCGTATTTCCGGGTATTTTGTGGGTATTTTGGGGGGCg                                                          >  2:1440459/1‑140 (MQ=255)
                                                                                cGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGGTGATGCAGGCGCAGAAAAAGGTTAAAAAAACCACGCTGCATCCTAAAACTTTCCGTATTCCGGGGTATCTGGTGGGGATTTTGGTGGGCGGTc                                                       >  2:392001/1‑140 (MQ=255)
                                                                                cGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGCGCAGAAAAAGGTTAAGAAAGCCACGCTGCATCCTAAATCTTTCCGTATTTCGGGGTATCTGGGGGGTATTTTGGTGGTCGATc                                                       >  2:279658/1‑140 (MQ=255)
                                                                                cGCATTGCGTATTGCCCAGGCGGTGGCCAATAAGCGGGGTAGTGTGATGATGCTGACGTAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTACGTCTTCCGGTTTATCTGGTGGATATGGTGGTGGTctgtc                                                       <  2:44936/140‑5 (MQ=255)
                                                                                            tGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAAccc                                           <  1:1072384/140‑1 (MQ=255)
                                                                                            tGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAAccc                                           <  1:699873/140‑1 (MQ=255)
                                                                                             gCCCAGGCGGTGCACAATAACGGCGGGATTTTGATGGTGCAGGGGCAGAAAAAGGTTAAGAAAACCACGCCGCATCCTAAATCTTTCCGTATTCCGGGGTATCTGGGGGGTATTTTGGTGGGCGGTCCGGGTCAAAcccc                                          >  2:853639/1‑139 (MQ=255)
                                                                                                   gCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTa                                    <  1:279658/140‑1 (MQ=255)
                                                                                                        gCACAATAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCg                               <  1:853639/140‑1 (MQ=255)
                                                                                                            aaTAACGGCGGTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGc                           <  2:966706/140‑1 (MQ=255)
                                                                                                                   gcggTATTTTGATGATGCAGGCGCAGAAAAAGGTTAAGAAAACCACGCTGCATCCTAAATCTGTTCGTATTTCGGGGTATCTTGTGGGTATTGTGGTGGTCGATCCGGGTCAAAACCAACTGTATGGCGGGGCGcccggt                    >  2:1224855/1‑136 (MQ=255)
                                                                                                                      gTATTGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAAc                 <  2:555867/140‑1 (MQ=255)
                                                                                                                         ttGTGATGATGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGc              <  1:287600/140‑1 (MQ=255)
                                                                                                                             gatgatGCAGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTa          <  1:469058/140‑1 (MQ=255)
                                                                                                                                     aGGCGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGt  <  1:529802/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGT  >  NC_000913/1776127‑1776399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑