Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,974,705	T→C	D207G (GAC→GGC)	cheA ←	chemotaxis protein CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,974,705	0	T	C	100.0%	201.0 / NA	58	D207G (GAC→GGC)	cheA	chemotaxis protein CheA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/28);  total (30/28)

CTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGAAAGGATA  >  NC_000913/1974572‑1974839                                                                                                                                      |                                                                                                                                       cTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCtt                                                                                                                                  >  2:1244990/1‑140 (MQ=255)          gCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGt                                                                                                                         <  1:861655/140‑1 (MQ=255)           ccGGTCGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTc                                                                                                                        >  1:888537/1‑140 (MQ=255)            cGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCg                                                                                                                       >  2:855456/1‑140 (MQ=255)            cGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCg                                                                                                                       <  2:1209410/140‑1 (MQ=255)            cGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCg                                                                                                                       <  1:603117/140‑1 (MQ=255)              gTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGcc                                                                                                                     <  2:1392940/140‑1 (MQ=255)               ttGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGccc                                                                                                                    <  1:1103105/140‑1 (MQ=255)               ttGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGccc                                                                                                                    <  2:373868/140‑1 (MQ=255)                 ggCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCgg                                                                                                                  <  1:74717/140‑1 (MQ=255)                      ttGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAata                                                                                                             <  1:487661/140‑1 (MQ=255)                                 ccAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGc                                                                                                  >  2:1164266/1‑140 (MQ=255)                                        aaGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCg                                                                                           <  1:855456/140‑1 (MQ=255)                                          gCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGcc                                                                                         <  2:549036/140‑1 (MQ=255)                                               ggtggggtggATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCttt                                                                                    >  1:336587/1‑140 (MQ=255)                                                gtggggtggATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTc                                                                                   >  2:421760/1‑140 (MQ=255)                                                  ggggtggATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTcac                                                                                 <  1:454751/140‑1 (MQ=255)                                                   gggtggATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTca                                                                                  >  1:1361086/1‑138 (MQ=255)                                                   gggtggATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTca                                                                                  <  2:1361086/138‑1 (MQ=255)                                                      tggATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACg                                                                             <  1:1244990/140‑1 (MQ=255)                                                             ttttGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTca                                                                                  <  2:754183/128‑1 (MQ=255)                                                             ttttGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTca                                                                                  >  1:754183/1‑128 (MQ=255)                                                             ttttGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTa                                                                      <  2:336587/140‑1 (MQ=255)                                                                       aCTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAg                                                            >  1:245485/1‑140 (MQ=255)                                                                           cTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGt                                                        <  1:1054784/140‑1 (MQ=255)                                                                             aCTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTcc                                                      <  1:1164266/140‑1 (MQ=255)                                                                                 tttCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACg                                                                   <  1:1051087/123‑1 (MQ=255)                                                                                 tttCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACg                                                                   >  2:1051087/1‑123 (MQ=255)                                                                                 tttCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTc                                                              >  2:163896/1‑128 (MQ=255)                                                                                 tttCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTc                                                              <  1:163896/128‑1 (MQ=255)                                                                                 tttCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTcc                                                      >  1:1190201/1‑136 (MQ=255)                                                                                 tttCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTcc                                                      <  2:1190201/136‑1 (MQ=255)                                                                                  ttCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGtt                                                 <  2:245485/140‑1 (MQ=255)                                                                                   tCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGttc                                                >  2:1181341/1‑140 (MQ=255)                                                                                    cAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGttct                                               >  2:562285/1‑140 (MQ=255)                                                                                          tAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTcc                                         <  1:828740/140‑1 (MQ=255)                                                                                             tCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTcc                                                      <  2:1110495/124‑1 (MQ=255)                                                                                             tCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTcc                                                      >  1:1110495/1‑124 (MQ=255)                                                                                             tCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCcagc                                      >  2:117205/1‑140 (MQ=255)                                                                                             tCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCcagc                                      <  1:1181341/140‑1 (MQ=255)                                                                                                   cGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCg                                >  1:859175/1‑140 (MQ=255)                                                                                                   cGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCg                                >  1:699147/1‑140 (MQ=255)                                                                                                   cGGCTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCg                                >  2:770890/1‑140 (MQ=255)                                                                                                      cTTCAATCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACt                             >  2:1071055/1‑140 (MQ=255)                                                                                                          aaTCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTccc                         <  2:1278884/140‑1 (MQ=255)                                                                                                            tCACAAAACAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCg                       <  1:117205/140‑1 (MQ=255)                                                                                                                    cAGAGTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAg               >  1:993793/1‑140 (MQ=255)                                                                                                                       agTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGgcg            >  2:181113/1‑140 (MQ=255)                                                                                                                       agTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGgcg            >  1:956281/1‑140 (MQ=255)                                                                                                                       agTACCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGgcg            >  1:613644/1‑140 (MQ=255)                                                                                                                          aCCGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGa         <  1:1071055/140‑1 (MQ=255)                                                                                                                           ccGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGaa        <  1:181113/140‑1 (MQ=255)                                                                                                                            cGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGaaa       >  2:789654/1‑140 (MQ=255)                                                                                                                            cGCTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGaaa       >  1:1052627/1‑140 (MQ=255)                                                                                                                              cTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGAAAgg     >  2:1420295/1‑140 (MQ=255)                                                                                                                              cTGTGATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGAAAgg     >  2:1389885/1‑140 (MQ=255)                                                                                                                                 tgATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGAAAGGATa  >  2:431996/1‑140 (MQ=255)                                                                                                                                 tgATGCCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGAAAGGATa  >  2:605853/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGAAAGGATA  >  NC_000913/1974572‑1974839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑