Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,063,707	G→A	A254V (GCT→GTT)	cobT ←	nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,063,707	0	G	A	100.0%	221.4 / NA	63	A254V (GCT→GTT)	cobT	nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/31);  total (32/31)

CTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCAATTTTATTAGC  >  NC_000913/2063570‑2063834                                                                                                                                          |                                                                                                                                cTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAgcaaca                                                                                                                               <  1:167468/140‑1 (MQ=255)       gcgcgcCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAAc                                                                                                                         <  1:221708/140‑1 (MQ=255)            ccTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCAc                                                                                                                    >  1:1124281/1‑140 (MQ=255)            ccTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCAc                                                                                                                    <  1:933949/140‑1 (MQ=255)            ccTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCAc                                                                                                                    <  2:174747/140‑1 (MQ=255)                tttCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCa                                                                                                                <  2:1124281/140‑1 (MQ=255)                       cGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTc                                                                                                         >  1:876009/1‑140 (MQ=255)                       cGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTc                                                                                                         >  2:413817/1‑140 (MQ=255)                           aGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGAc                                                                                                     <  2:876009/140‑1 (MQ=255)                                       aTGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACcaaatcaaa                                                                                            >  1:1396014/1‑137 (MQ=255)                                       aTGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACcaaatcaaa                                                                                            <  2:1396014/137‑1 (MQ=255)                                       aTGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATcc                                                                                         >  1:183456/1‑140 (MQ=255)                                         gagaTACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATccac                                                                                       <  2:389896/140‑1 (MQ=255)                                          agaTACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGAc                                                                                                     >  2:1176386/1‑125 (MQ=255)                                          agaTACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGAc                                                                                                     <  1:1176386/125‑1 (MQ=255)                                            aTACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATccaccca                                                                                    >  1:1354974/1‑140 (MQ=255)                                            aTACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATccaccca                                                                                    <  2:693944/140‑1 (MQ=255)                                                    tGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGcc                                                                            >  1:174172/1‑140 (MQ=255)                                                      aTCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATccac                                                                                       <  2:1158460/127‑1 (MQ=255)                                                      aTCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATccac                                                                                       >  1:1158460/1‑127 (MQ=255)                                                      aTCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAg                                                                          <  2:174172/140‑1 (MQ=255)                                                         gCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGAc                                                                       <  2:1354974/140‑1 (MQ=255)                                                           aGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACAt                                                                     >  2:1300719/1‑140 (MQ=255)                                                           aGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACAt                                                                     <  2:19849/140‑1 (MQ=255)                                                           aGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACAt                                                                     <  1:457177/140‑1 (MQ=255)                                                                aCATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAaca                                                                >  2:1282982/1‑140 (MQ=255)                                                                aCATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAaca                                                                >  2:929247/1‑140 (MQ=255)                                                                aCATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAaca                                                                <  2:1466462/140‑1 (MQ=255)                                                                  aTCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACAcc                                                              <  1:82315/140‑1 (MQ=255)                                                                  aTCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACAcc                                                              >  1:1252456/1‑140 (MQ=255)                                                                  aTCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACAcc                                                              <  1:1282982/140‑1 (MQ=255)                                                                  aTCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACAcc                                                              >  2:1120366/1‑140 (MQ=255)                                                                      ggCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATcc                                                          <  2:1252456/140‑1 (MQ=255)                                                                       gCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCt                                                         <  2:494143/140‑1 (MQ=255)                                                                         aGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACAt                                                                     >  2:1080020/1‑126 (MQ=255)                                                                         aGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACAt                                                                     <  1:1080020/126‑1 (MQ=255)                                                                         aGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGa                                                       >  1:1248985/1‑140 (MQ=255)                                                                           gCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAgg                                                     >  1:700866/1‑140 (MQ=255)                                                                            cTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGa                                                    >  1:649362/1‑140 (MQ=255)                                                                             tGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGAt                                                   >  2:906512/1‑140 (MQ=255)                                                                              gCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGAtt                                                  >  2:290949/1‑140 (MQ=255)                                                                              gCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATCCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGAtt                                                  >  2:99627/1‑140 (MQ=255)                                                                               cGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGAttt                                                 >  1:351565/1‑140 (MQ=255)                                                                                  gcgcAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGt                                              <  1:929247/140‑1 (MQ=255)                                                                                   cgcAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGtt                                             <  2:1248985/140‑1 (MQ=255)                                                                                      agcagcaTAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGAt                                          >  1:474767/1‑140 (MQ=255)                                                                                                  aagaaaTCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCg                              >  2:36506/1‑140 (MQ=255)                                                                                                    gaaaTCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGATCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGcc                            <  2:190276/140‑1 (MQ=255)                                                                                                    gaaaTCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGcc                            <  2:183456/140‑1 (MQ=255)                                                                                                     aaaTCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGccc                           >  2:1297362/1‑140 (MQ=255)                                                                                                     aaaTCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGcc                            <  1:1111231/139‑1 (MQ=255)                                                                                                     aaaTCCATCCAGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGcc                            >  2:1111231/1‑139 (MQ=255)                                                                                                        tccatccaGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGAc                        >  1:635637/1‑140 (MQ=255)                                                                                                            tccaGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGcac                    <  2:649362/140‑1 (MQ=255)                                                                                                               aGCAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCacaac                 >  1:162357/1‑140 (MQ=255)                                                                                                                 cAACACGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACAt               >  2:1195555/1‑140 (MQ=255)                                                                                                                    cacGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCaa            <  1:562721/140‑1 (MQ=255)                                                                                                                    cacGGGTAAACCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCaa            >  2:562721/1‑140 (MQ=255)                                                                                                                            aaCCGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCAATTttatta    <  1:1300719/140‑1 (MQ=255)                                                                                                                              ccGCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCAATTTTATTAGc  <  2:1140012/140‑1 (MQ=255)                                                                                                                                gCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCAATTtta       <  2:1140381/133‑1 (MQ=255)                                                                                                                                gCAGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCAATTtta       >  1:1140381/1‑133 (MQ=255)                                                                                                                                  aGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCAATTTTATTAGc  >  1:489198/1‑136 (MQ=255)                                                                                                                                  aGGAAGCAACACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCAATTTTATTAGc  <  2:489198/136‑1 (MQ=255)                                                                                                                                          |                                                                                                                                CTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGGATTTGGTTGATTCAACGTAATCGCCCGACGCACAACATCAATTTTATTAGC  >  NC_000913/2063570‑2063834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑