Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	132,467	A→G	T285A (ACG→GCG)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	132,467	0	A	G	100.0%	174.2 / NA	54	T285A (ACG→GCG)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/31);  total (23/31)

TTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAAC  >  NC_000913/132330‑132602                                                                                                                                          |                                                                                                                                         ttGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAAcgcg                                                                                                                                        >  1:232272/1‑140 (MQ=255) ttGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAAcgcg                                                                                                                                        >  2:682283/1‑140 (MQ=255)       gTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGaa                                                                                                                                  <  1:808631/140‑1 (MQ=255)        tACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAg                                                                                                                                 >  1:1063065/1‑140 (MQ=255)            gttttttcGCTAAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGAcgc                                                                                                                             <  2:373589/135‑1 (MQ=255)              ttcttcGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCgg                                                                                                                           <  2:76174/140‑1 (MQ=255)              ttcttcGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCgg                                                                                                                           >  2:444634/1‑140 (MQ=255)              ttcttcGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGGAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCgg                                                                                                                           >  2:1306185/1‑140 (MQ=255)                cttcGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCgggg                                                                                                                         >  1:629539/1‑139 (MQ=255)                  tcGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGc                                                                                                                       >  1:188467/1‑140 (MQ=255)                       tAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGc                                                                                                                  >  1:922845/1‑140 (MQ=255)                       tAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGc                                                                                                                  <  1:739551/140‑1 (MQ=255)                               ccACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTTTTTAACGCGATGGAAGACGCGGGGGGACTGCCAATCGaa                                                                                                          >  2:672694/1‑140 (MQ=255)                               ccACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCaa                                                                                                               <  2:614049/135‑1 (MQ=255)                               ccACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCaa                                                                                                               >  1:614049/1‑135 (MQ=255)                               ccACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCGATCGaa                                                                                                          >  1:1336390/1‑140 (MQ=255)                                    aaCTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGa                                                                                                     <  2:1174685/140‑1 (MQ=255)                                    aaCTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGa                                                                                                     <  1:672694/140‑1 (MQ=255)                                     aCTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGAc                                                                                                    <  1:682283/140‑1 (MQ=255)                                         cGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGtct                                                                                                <  1:1455069/140‑1 (MQ=255)                                         cGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGtct                                                                                                <  2:922845/140‑1 (MQ=255)                                         cGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGtct                                                                                                <  1:113755/140‑1 (MQ=255)                                          gTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAAACGAAATCGACGtctc                                                                                               >  1:152753/1‑140 (MQ=255)                                          gTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGGGCACTGCCAATCGAAGTCGACGtctc                                                                                               >  1:89848/1‑140 (MQ=255)                                           ttCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGGGCACTGCCAATCGAAGTCGACGTctct                                                                                              >  1:731913/1‑140 (MQ=255)                                              tgtgGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AAc                                                                                          <  1:793443/140‑1 (MQ=255)                                               gtgGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGGGCACTGCCAATCGAAGTCGACGTCTCT‑AAcc                                                                                         >  2:443653/1‑140 (MQ=255)                                                tgGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCt                                                                                        >  1:667340/1‑140 (MQ=255)                                                     tATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACa                                                                                   <  1:1396818/140‑1 (MQ=255)                                                          gCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AAc                                                                                          <  1:176206/128‑1 (MQ=255)                                                          gCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGGGCACTGCCAATCGAAGTCGACGTCTCT‑AAc                                                                                          >  2:176206/1‑128 (MQ=255)                                                           cGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCg                                                                             <  2:188467/140‑1 (MQ=255)                                                            gatgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGa                                                                            <  1:600535/140‑1 (MQ=255)                                                             atgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGGAGACGCGGGGGCACTGCCAATCGAAGTCGGCCTCCCT‑AACCTGAACATGGGCGAc                                                                           >  2:374675/1‑140 (MQ=255)                                                             atgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAAATGCACCCATCTTCTTTAAAGCGGTGGGAGACGCGGGGGGACTGCCCAACCGAGTCGGCCGCTCTAAACCTGAAAATgggggc                                                                            >  2:1455197/1‑137 (MQ=255)                                                             atgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGGAAAATTGCACCCATCTTCTTTAACGCGAAGGAAGACGCGGGGGCAATGCCAAACGAAGTCGCCGTGCCT‑AACCCGGACATGGGCGAc                                                                           >  2:1205050/1‑140 (MQ=255)                                                              tgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACg                                                                          <  2:784807/140‑1 (MQ=255)                                                              tgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACg                                                                          >  1:784807/1‑140 (MQ=255)                                                                  atTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCGATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGAt                                                                      <  2:1336390/140‑1 (MQ=255)                                                                   ttCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGAtt                                                                     >  1:1365738/1‑140 (MQ=255)                                                                        caTGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGt                                                                >  2:585763/1‑140 (MQ=255)                                                                        caTGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGt                                                                >  2:1320163/1‑140 (MQ=255)                                                                           gtgCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAAAGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTTACGTTTa                                                             >  2:876715/1‑140 (MQ=255)                                                                              ccGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGa                                                                            >  2:995907/1‑122 (MQ=255)                                                                              ccGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGa                                                                            <  1:995907/122‑1 (MQ=255)                                                                              ccGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGAt                                                                      <  1:1146767/128‑1 (MQ=255)                                                                              ccGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGAt                                                                      >  2:1146767/1‑128 (MQ=255)                                                                                gAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGGGCACTGCCCATCGAAGTCGACGGCTCT‑AACCCGAAAAAGGGGGGCGTGGTTGACGttttaccgc                                                        >  2:1446059/1‑134 (MQ=255)                                                                                 aaCAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTa                                                       <  2:667340/140‑1 (MQ=255)                                                                                 aaCAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTa                                                       <  2:1063065/140‑1 (MQ=255)                                                                                 aaCAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTa                                                       <  1:1052866/140‑1 (MQ=255)                                                                                  aCAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTa                                                       >  1:19728/1‑139 (MQ=255)                                                                                  aCAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTa                                                       <  2:19728/139‑1 (MQ=255)                                                                                      aCGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTa                                                       >  1:20761/1‑135 (MQ=255)                                                                                      aCGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTa                                                       <  2:20761/135‑1 (MQ=255)                                                                                      aCGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAg                                                  >  1:972682/1‑140 (MQ=255)                                                                                         cggcggTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTg                                               <  1:1205050/140‑1 (MQ=255)                                                                                                ggtTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCg                                        <  1:585763/140‑1 (MQ=255)                                                                                                            ggcggTAAAATTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGGGAAGTGCGTAACCACGAAAc                            >  1:1297293/1‑140 (MQ=255)                                                                                                                    aaTTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAAc                    <  1:1306185/140‑1 (MQ=255)                                                                                                                    aaTTGCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAAc                    <  1:444634/140‑1 (MQ=255)                                                                                                                        gCACCCATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAActgct                >  2:12052/1‑140 (MQ=255)                                                                                                                           cccATCTTCTTTAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGc             <  2:1403199/140‑1 (MQ=255)                                                                                                                                     ttAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGaa   <  1:12052/140‑1 (MQ=255)                                                                                                                                      tAACGCGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACATGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAAc  >  1:500289/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCT‑AACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAAC  >  NC_000913/132330‑132602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑