Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,219,789	T→C	N734S (AAT→AGT)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,219,789	0	T	C	100.0%	238.4 / NA	69	N734S (AAT→AGT)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/36);  total (33/36)

GGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGATTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACAT  >  NC_000913/2219652‑2219925                                                                                                                                          |                                                                                                                                         ggCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTc                                                                                                                                        >  1:782245/1‑140 (MQ=255)    cTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAg                                                                                                                                     >  1:1015696/1‑140 (MQ=255)            ggCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAg                                                                                                                             <  1:598790/140‑1 (MQ=255)            ggCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAg                                                                                                                             <  2:14254/140‑1 (MQ=255)            ggCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAg                                                                                                                             >  2:226548/1‑140 (MQ=255)            ggCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAg                                                                                                                             <  2:782245/140‑1 (MQ=255)            ggCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAg                                                                                                                             <  2:918707/140‑1 (MQ=255)            ggCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAg                                                                                                                             <  1:112396/140‑1 (MQ=255)                 aaaCGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCt                                                                                                                        <  1:767571/140‑1 (MQ=255)                       cgcgCCCTTACTAAAGCACTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCATAACTTCAg                                                                                                                             >  1:192443/1‑129 (MQ=255)                       cgcgCCCTTACTAAAGCACTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCATAACTTCAg                                                                                                                             <  2:192443/129‑1 (MQ=255)                          gcCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATatcga                                                                                                               <  1:78199/140‑1 (MQ=255)                            ccTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATatcgatc                                                                                                             <  2:1379777/140‑1 (MQ=255)                            ccTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATatcgatc                                                                                                             <  2:1015696/140‑1 (MQ=255)                            ccTTACTAAAGCATTACAGCAACACAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATatcgatc                                                                                                             <  1:955832/140‑1 (MQ=255)                                     aGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTg                                                                                                    >  1:438622/1‑140 (MQ=255)                                        aTTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACa                                                                                                 >  2:186141/1‑140 (MQ=255)                                        aTTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACa                                                                                                 >  2:1427568/1‑140 (MQ=255)                                          tACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGt                                                                                               >  2:916393/1‑140 (MQ=255)                                          tACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGt                                                                                               >  2:337030/1‑140 (MQ=255)                                           aCAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTc                                                                                              <  1:526986/140‑1 (MQ=255)                                            cAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCt                                                                                             >  1:639120/1‑140 (MQ=255)                                                 aCTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGtt                                                                                        >  2:11983/1‑140 (MQ=255)                                                 aCTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGtt                                                                                        >  1:577700/1‑140 (MQ=255)                                                      aaCTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGcc                                                                                   <  1:533193/140‑1 (MQ=255)                                                      aaCTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGcc                                                                                   <  1:916393/140‑1 (MQ=255)                                                      aaCTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGcc                                                                                   <  1:1427568/140‑1 (MQ=255)                                                       aCTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGccc                                                                                  >  1:708599/1‑140 (MQ=255)                                                                     aCGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGAt                                                                    <  2:452896/140‑1 (MQ=255)                                                                          tGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAATTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTtct                                                               >  2:120079/1‑140 (MQ=255)                                                                          tGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTtct                                                               >  1:556387/1‑140 (MQ=255)                                                                          tGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTtct                                                               >  2:949673/1‑140 (MQ=255)                                                                          tGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTtct                                                               >  1:1200772/1‑140 (MQ=255)                                                                          tGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTtct                                                               >  1:865451/1‑140 (MQ=255)                                                                          tGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTtct                                                               >  1:764398/1‑140 (MQ=255)                                                                          tGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTtct                                                               >  1:458320/1‑140 (MQ=255)                                                                                     tGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTc                                                    >  2:887369/1‑140 (MQ=255)                                                                                       ccGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAg                                                  >  1:717367/1‑140 (MQ=255)                                                                                         gATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTc                                                    >  1:1069997/1‑136 (MQ=255)                                                                                         gATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTc                                                    <  2:1069997/136‑1 (MQ=255)                                                                                             aaGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGttt                                            >  1:1032500/1‑140 (MQ=255)                                                                                               gACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTca                                          <  2:458320/140‑1 (MQ=255)                                                                                               gACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTca                                          >  2:231635/1‑140 (MQ=255)                                                                                                    tGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAggg                                     >  1:1308342/1‑140 (MQ=255)                                                                                                    tGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAggg                                     <  1:741480/140‑1 (MQ=255)                                                                                                    tGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAggg                                     <  1:231635/140‑1 (MQ=255)                                                                                                     gAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGc                                    <  1:186141/140‑1 (MQ=255)                                                                                                       aCTTGCCAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGa                                  <  2:556387/140‑1 (MQ=255)                                                                                                            ccAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGc                                    >  1:224058/1‑133 (MQ=255)                                                                                                            ccAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGc                                    <  2:224058/133‑1 (MQ=255)                                                                                                            ccAGGCTCGGCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCAt                             >  2:985338/1‑140 (MQ=255)                                                                                                                    ggCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCg                     >  1:219746/1‑140 (MQ=255)                                                                                                                     gCGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGt                    >  1:1086740/1‑140 (MQ=255)                                                                                                                      cGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTc                   >  1:1271793/1‑140 (MQ=255)                                                                                                                      cGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTc                   <  1:949673/140‑1 (MQ=255)                                                                                                                      cGTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTc                   <  2:479631/140‑1 (MQ=255)                                                                                                                       gTCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTca                  <  2:1308342/140‑1 (MQ=255)                                                                                                                        tCATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTcac                 <  1:337030/140‑1 (MQ=255)                                                                                                                         cATATTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTcaca                <  2:829829/140‑1 (MQ=255)                                                                                                                          atatTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACAt               >  2:671375/1‑140 (MQ=255)                                                                                                                          atatTTCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACAt               <  2:1200772/140‑1 (MQ=255)                                                                                                                             tttCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCt            <  1:226548/140‑1 (MQ=255)                                                                                                                             tttCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCt            <  2:295153/140‑1 (MQ=255)                                                                                                                               tCATCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACAACCTGc          <  2:577700/140‑1 (MQ=255)                                                                                                                                  tCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAg       <  2:708599/140‑1 (MQ=255)                                                                                                                                  tCTGTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAg       <  1:887369/140‑1 (MQ=255)                                                                                                                                     gTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTAc    <  2:764398/140‑1 (MQ=255)                                                                                                                                     gTTGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTAc    <  1:671375/140‑1 (MQ=255)                                                                                                                                      ttGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACa   >  1:651820/1‑140 (MQ=255)                                                                                                                                       tGACTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACAt  <  2:517019/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGATTCCAGAACTTCAGCGCCTCAATATCGATCGGGAAGCTGACAGTCTGAGTTTCGCCCGGTTTCAGGGTGATTTTCTCAAAGCCTTTCAGCTGTTTCACAGGGCGACTCATGGAAGCCGTCACATCCTGCAAGTACAT  >  NC_000913/2219652‑2219925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑