Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,869	G→A	G413E (GGA→GAA)	radD →	putative DNA repair helicase RadD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,869	0	G	A	100.0%	185.0 / NA	53	G413E (GGA→GAA)	radD	putative DNA repair helicase RadD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/26);  total (27/26)

TGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATC  >  NC_000913/2281735‑2282003                                                                                                                                       |                                                                                                                                       tGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGt                                                                                                                                   <  1:1274639/140‑1 (MQ=255)    tACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTg                                                                                                                                <  1:992498/140‑1 (MQ=255)       acCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAg                                                                                                                             >  1:1465821/1‑140 (MQ=255)          aaaaGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATg                                                                                                                          >  2:955448/1‑140 (MQ=255)           aaaGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTg                                                                                                                                <  2:996042/133‑1 (MQ=255)           aaaGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTg                                                                                                                                >  1:996042/1‑133 (MQ=255)           aaaGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATga                                                                                                                         <  1:610004/140‑1 (MQ=255)             aGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATgacg                                                                                                                       <  2:811833/140‑1 (MQ=255)              ggCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATgacga                                                                                                                      >  2:792215/1‑140 (MQ=255)                 aaaaGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACgg                                                                                                                   <  2:418377/140‑1 (MQ=255)                 aaaaGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACgg                                                                                                                   <  2:96899/140‑1 (MQ=255)                     gTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCAt                                                                                                               <  1:219752/140‑1 (MQ=255)                         cAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATcgcg                                                                                                           <  2:294437/140‑1 (MQ=255)                         cAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATcgcg                                                                                                           <  1:1209401/140‑1 (MQ=255)                             gTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACa                                                                                                       <  1:792215/140‑1 (MQ=255)                                     aCAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACt                                                                                               <  1:334646/140‑1 (MQ=255)                                     aCAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACt                                                                                               <  2:877590/140‑1 (MQ=255)                                     aCAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACt                                                                                               <  1:123436/140‑1 (MQ=255)                                         gTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAAt                                                                                                     <  1:824577/130‑1 (MQ=255)                                         gTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAAt                                                                                                     >  2:824577/1‑130 (MQ=255)                                         gTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACttccg                                                                                           >  2:41337/1‑140 (MQ=255)                                                          cGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAAtt                                                                          <  1:201954/140‑1 (MQ=255)                                                            gTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGc                                                                        <  1:41337/140‑1 (MQ=255)                                                               ttGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCg                                                                     >  2:43039/1‑140 (MQ=255)                                                                tGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGc                                                                    >  2:136167/1‑140 (MQ=255)                                                                tGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGc                                                                    >  2:989098/1‑140 (MQ=255)                                                                       acCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTaa                                                             >  2:276971/1‑140 (MQ=255)                                                                              gggggAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGaa                                                      <  1:1067581/140‑1 (MQ=255)                                                                                gggAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGaaaa                                                    >  2:647215/1‑140 (MQ=255)                                                                                  gAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAGTGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACg                                                  <  2:820491/140‑1 (MQ=255)                                                                                   aaaaCGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGa                                                 >  2:739702/1‑140 (MQ=255)                                                                                   aaaaCGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGa                                                 >  1:591679/1‑140 (MQ=255)                                                                                    aaaCGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGat                                                >  2:299725/1‑140 (MQ=255)                                                                                             ccgACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTgccgcc                                       <  1:683493/140‑1 (MQ=255)                                                                                                         tgattgaACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCcgc                           <  1:136167/140‑1 (MQ=255)                                                                                                         tgattaaACACTTGGGTCGTCGCTGTCAGGAATGGTTTGAAGACGACGACGGTCATCGCGAACAATGTGACTTCCCTTTCCGTTTAAAAATTTCCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCcgc                           <  2:337202/140‑1 (MQ=255)                                                                                                            ttgaACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGaa                        >  1:337202/1‑140 (MQ=255)                                                                                                              gaACATTTTGGTCGTCGCTGTCAGGAATGTTTTGAAAATGCCGACGGTCATCGCGAACAATGTGCCTTCCTTTTCCTTTTAAAAAATTCCCCGCAATGTAACGCGGAAAACGATATTGCCCCCCGCCGCTGCCGCGAAtg                      <  2:1017616/140‑1 (MQ=255)                                                                                                              gaACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                      >  1:145920/1‑140 (MQ=255)                                                                                                              gaACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                      >  1:113001/1‑140 (MQ=255)                                                                                                              gaACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                      <  1:43039/140‑1 (MQ=255)                                                                                                              gaACACTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                      <  1:276971/140‑1 (MQ=255)                                                                                                                acacTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtgtg                    >  2:379113/1‑140 (MQ=255)                                                                                                                 ggtttgggTCGTCGCTGTAAGGAATGGTTGAAAGATGACGACGGTCATCGCAAAAAATGTGCCTTCCGTTTCCTTTTTAAAAATTGCCCGAAATGTAACGCGAAAAACGTTTTTGCCGCCCGCCGCTGCCGCGAATGTGa                   <  2:728932/134‑1 (MQ=255)                                                                                                                 cacTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGa                   >  2:760187/1‑140 (MQ=255)                                                                                                                   ttttGGTCGTCGTTGTCAGGAATGGTTTGAAGATGACGGCGGTCATCGCGAACAATGTGACTTCCGTTCCCGTTTTAAAAATTCCCCCCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGaca                 <  2:32642/139‑1 (MQ=255)                                                                                                                   cTTTGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGaca                 <  1:760187/140‑1 (MQ=255)                                                                                                                     ttGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACAcc               >  2:1233780/1‑140 (MQ=255)                                                                                                                      tGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                      >  1:99603/1‑132 (MQ=255)                                                                                                                      tGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                      <  2:99603/132‑1 (MQ=255)                                                                                                                      tGGTCGTCGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCg              >  1:1017616/1‑140 (MQ=255)                                                                                                                        gtcgtcGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTa            <  1:299725/140‑1 (MQ=255)                                                                                                                        gtcgtcGCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTa            <  1:261429/140‑1 (MQ=255)                                                                                                                         tcgtcgCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTAc           >  1:312140/1‑140 (MQ=255)                                                                                                                           gtcgCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTg         >  1:726378/1‑140 (MQ=255)                                                                                                                            tcgCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTgg        >  2:211765/1‑140 (MQ=255)                                                                                                                              gCTGTCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTggct      >  1:728932/1‑138 (MQ=255)                                                                                                                                  tCAGGAATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCACAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATc  >  1:90395/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       TGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATC  >  NC_000913/2281735‑2282003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑