Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,356,131	T→C	V204A (GTA→GCA)	glpC →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,356,131	0	T	C	100.0%	337.5 / NA	100	V204A (GTA→GCA)	glpC	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (58/42);  total (58/42)

TATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGC  >  NC_000913/2355995‑2356265                                                                                                                                         |                                                                                                                                       tataAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCAcc                                                                                                                                     >  2:1318297/1‑140 (MQ=255)      aGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTaa                                                                                                                                >  1:1406472/1‑140 (MQ=255)       gACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAAt                                                                                                                               >  2:315380/1‑140 (MQ=255)         ccAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCg                                                                                                                             >  1:1102729/1‑140 (MQ=255)         ccAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCg                                                                                                                             >  1:827186/1‑140 (MQ=255)         ccAGGTCGCTTTCTTTCACGCCTGCTTCGTTAACTACAACCATCCGCAGTTAGGGAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCCCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCg                                                                                                                             >  1:1039208/1‑140 (MQ=255)           aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGcc                                                                                                                           >  1:571536/1‑140 (MQ=255)           aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGcc                                                                                                                           >  2:242730/1‑140 (MQ=255)           aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGcc                                                                                                                           >  2:549373/1‑140 (MQ=255)           aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGcc                                                                                                                           >  1:370483/1‑140 (MQ=255)           aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGcc                                                                                                                           >  1:900487/1‑140 (MQ=255)           aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGcc                                                                                                                           >  1:278405/1‑140 (MQ=255)            ggTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCa                                                                                                                          <  2:370483/140‑1 (MQ=255)            ggTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCa                                                                                                                          <  2:900487/140‑1 (MQ=255)               cGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACg                                                                                                                       <  2:1102729/140‑1 (MQ=255)                  tttctttcACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCt                                                                                                                    >  2:646911/1‑140 (MQ=255)                   ttctttcACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCtt                                                                                                                   <  2:571536/140‑1 (MQ=255)                   ttctttcACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCtt                                                                                                                   <  2:820421/140‑1 (MQ=255)                            ggCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATaa                                                                                                          <  2:1192980/140‑1 (MQ=255)                                 ctTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCAc                                                                                                     >  2:1317531/1‑140 (MQ=255)                                           tacgaCCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGc                                                                                           >  1:60883/5‑140 (MQ=255)                                            aCAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCa                                                                                          <  2:827186/140‑1 (MQ=255)                                            aCAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCa                                                                                          >  2:221070/1‑140 (MQ=255)                                              aaCCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAAt                                                                                        <  1:221070/140‑1 (MQ=255)                                               aCCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAAtt                                                                                       >  2:558089/1‑140 (MQ=255)                                                 cATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTAc                                                                                     <  2:486234/140‑1 (MQ=255)                                                 cATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTAc                                                                                     <  2:691681/140‑1 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCa                                                                                          >  2:63827/1‑133 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCa                                                                                          <  1:63827/133‑1 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTAc                                                                                     <  1:530844/138‑1 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTAc                                                                                     >  2:530844/1‑138 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGa                                                                                   >  1:573973/1‑140 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGa                                                                                   >  2:1275379/1‑140 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGa                                                                                   >  2:1417025/1‑140 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGa                                                                                   <  1:385307/140‑1 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGa                                                                                   >  2:570146/1‑140 (MQ=255)                                                   tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGa                                                                                   <  2:573973/140‑1 (MQ=255)                                                    ccGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGaa                                                                                  >  1:605115/1‑140 (MQ=255)                                                    ccGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGaa                                                                                  >  2:1325125/1‑140 (MQ=255)                                                     cGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAAt                                                                                 >  1:30750/1‑140 (MQ=255)                                                     cGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAAt                                                                                 >  1:640430/1‑140 (MQ=255)                                                      gCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATg                                                                                <  1:558089/140‑1 (MQ=255)                                                      gCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATg                                                                                <  1:549373/140‑1 (MQ=255)                                                          ttAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTaga                                                                            >  2:631435/1‑140 (MQ=255)                                                                aaaGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGAt                                                                      <  1:242730/140‑1 (MQ=255)                                                                  aGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATcc                                                                    <  2:30750/140‑1 (MQ=255)                                                                   gATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCcg                                                                   <  1:646911/140‑1 (MQ=255)                                                                   gATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCcg                                                                   <  2:1039208/140‑1 (MQ=255)                                                                    aTTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCcgc                                                                  >  2:414515/1‑140 (MQ=255)                                                                    aTTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCcgc                                                                  >  2:1059025/1‑140 (MQ=255)                                                                    aTTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCcgc                                                                  <  1:734079/140‑1 (MQ=255)                                                                    aTTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCcgc                                                                  <  1:1318297/140‑1 (MQ=255)                                                                        aattaaAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAg                                                              <  1:631435/140‑1 (MQ=255)                                                                              aGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTgg                                                        >  2:1222414/1‑140 (MQ=255)                                                                                  cTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCGAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGt                                                    >  1:405677/1‑140 (MQ=255)                                                                                  cTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGt                                                    >  1:1036695/1‑140 (MQ=255)                                                                                   tCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCGCGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTa                                                   <  2:1406472/140‑1 (MQ=255)                                                                                   tCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTgg                                                        >  1:780907/1‑135 (MQ=255)                                                                                   tCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTgg                                                        <  2:780907/135‑1 (MQ=255)                                                                                     aaCGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTaaa                                                 <  2:605115/140‑1 (MQ=255)                                                                                       cGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAg                                               >  2:931660/1‑140 (MQ=255)                                                                                       cGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAg                                               >  2:1283045/1‑140 (MQ=255)                                                                                       cGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAg                                               >  1:112834/1‑140 (MQ=255)                                                                                               gTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCg                                       >  1:217787/1‑140 (MQ=255)                                                                                                tACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCgg                                      <  1:1317531/140‑1 (MQ=255)                                                                                                tACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCgg                                      <  1:315380/140‑1 (MQ=255)                                                                                                 aCCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCCATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGt                                     >  1:693430/1‑140 (MQ=255)                                                                                                      tgtACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTg                                >  1:171754/1‑140 (MQ=255)                                                                                                        tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGcc                              >  1:990745/1‑140 (MQ=255)                                                                                                        tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGcc                              <  1:1283045/140‑1 (MQ=255)                                                                                                        tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGcc                              >  1:124028/1‑140 (MQ=255)                                                                                                        tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGcc                              >  2:883612/1‑140 (MQ=255)                                                                                                        tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGcc                              >  1:773012/1‑140 (MQ=255)                                                                                                          cAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAc                            >  1:402355/1‑140 (MQ=255)                                                                                                          cAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAc                            >  1:629021/1‑140 (MQ=255)                                                                                                             cTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                             >  1:1426796/1‑136 (MQ=255)                                                                                                             cTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                             <  2:1426796/136‑1 (MQ=255)                                                                                                             cTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctc                         >  2:117665/1‑140 (MQ=255)                                                                                                             cTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctc                         >  2:234862/1‑140 (MQ=255)                                                                                                             cTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctc                         >  2:1191941/1‑140 (MQ=255)                                                                                                              tGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctcc                        <  1:931660/140‑1 (MQ=255)                                                                                                               gCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctcct                       <  1:1417025/140‑1 (MQ=255)                                                                                                                cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCgg                                      >  1:1318258/1‑124 (MQ=255)                                                                                                                cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCgg                                      <  2:1318258/124‑1 (MQ=255)                                                                                                                cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                      <  1:414515/140‑1 (MQ=255)                                                                                                                cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                      >  1:952389/1‑140 (MQ=255)                                                                                                                cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                      <  1:234862/140‑1 (MQ=255)                                                                                                                cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                      <  2:60883/140‑1 (MQ=255)                                                                                                                cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGAT‑AAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCa                     >  2:243055/1‑140 (MQ=255)                                                                                                                cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGAT‑AAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCa                     <  1:243055/140‑1 (MQ=255)                                                                                                                 tCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCa                     <  2:1036695/140‑1 (MQ=255)                                                                                                                  cAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCaa                    >  2:1202050/1‑140 (MQ=255)                                                                                                                      aaaGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTg                <  2:124028/140‑1 (MQ=255)                                                                                                                      aaaGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTg                <  2:753540/140‑1 (MQ=255)                                                                                                                      aaaGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTg                <  2:171754/140‑1 (MQ=255)                                                                                                                                gctgcGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCCATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGcc      <  2:693430/140‑1 (MQ=255)                                                                                                                                  tgcGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCt    >  1:250649/1‑140 (MQ=255)                                                                                                                                    cggcgCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTgc  >  2:622178/1‑140 (MQ=255)                                                                                                                                    cggcgCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTgc  >  1:56824/1‑140 (MQ=255)                                                                                                                                    cggcgCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTgc  >  1:1004720/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGC  >  NC_000913/2355995‑2356265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑