Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,102,340	(C)6→5	coding (513/720 nt)	trmI ←	tRNA m(7)G46 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,102,335	0	C	.	100.0%	286.2 / NA	67	coding (518/720 nt)	trmI	tRNA m(7)G46 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (34/33);  total (34/33)

CGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATT  >  NC_000913/3102202‑3102469                                                                                                                                      |                                                                                                                                       cGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGcccccag                                                                                                                                  <  2:21947/140‑3 (MQ=255)  gTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGcc                                                                                                                                       <  2:300018/134‑1 (MQ=255)  gTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGcc                                                                                                                                       >  1:300018/1‑134 (MQ=255)         gACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGtt                                                                                                                         <  2:198049/140‑1 (MQ=255)                 gCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCCGTTTGccgaac                                                                                                                 >  2:277928/1‑135 (MQ=255)                    ggACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTAcc                                                                                                              >  1:401180/1‑140 (MQ=255)                     gACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGtt                                                                                                                         >  2:879957/1‑128 (MQ=255)                     gACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGtt                                                                                                                         <  1:879957/128‑1 (MQ=255)                      aCGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAg                                                                                                            >  2:632292/1‑140 (MQ=255)                       cgcgGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGt                                                                                                           >  1:940552/1‑140 (MQ=255)                                 aaTCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACg                                                                                                 <  2:640612/140‑1 (MQ=255)                                  aTCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                                <  2:634248/140‑1 (MQ=255)                                   tCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGc                                                                                               <  1:559428/140‑1 (MQ=255)                                     aTTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAc                                                                                             >  1:887435/1‑140 (MQ=255)                                      ttGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                            >  1:836151/1‑140 (MQ=255)                                        gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCaaa                                                                                                   <  1:400269/131‑1 (MQ=255)                                        gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCaaa                                                                                                   >  2:400269/1‑131 (MQ=255)                                        gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTg                                                                                          >  1:1003921/1‑140 (MQ=255)                                          tctctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCGTATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGag                                                                                        >  1:763911/1‑139 (MQ=255)                                          tctctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGaa                                                                                        <  2:319784/140‑1 (MQ=255)                                          tctctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGaa                                                                                        <  2:726725/140‑1 (MQ=255)                                           ctctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAAc                                                                                       >  2:349741/1‑140 (MQ=255)                                              tGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGAt                                                                                    <  1:632292/140‑1 (MQ=255)                                               gACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATa                                                                                   <  2:940552/140‑1 (MQ=255)                                                aCAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATAc                                                                                  <  2:391142/140‑1 (MQ=255)                                                aCAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATAc                                                                                  <  1:683484/140‑1 (MQ=255)                                                aCAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATAc                                                                                  >  1:433192/1‑140 (MQ=255)                                                 cAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACg                                                                                 <  1:429401/140‑1 (MQ=255)                                                 cAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACg                                                                                 <  1:42080/140‑1 (MQ=255)                                                 cAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACg                                                                                 <  2:979448/140‑1 (MQ=255)                                                    gTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCg                                                                              >  2:717138/1‑140 (MQ=255)                                                     tttttATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCGTATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAGCGATACGGCGt                                                                             <  2:763911/140‑1 (MQ=255)                                                                gTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCAGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgcg                                                                  >  2:45907/1‑140 (MQ=255)                                                                 tCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgcgc                                                                 >  2:18390/1‑140 (MQ=255)                                                                  cAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGTTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgcgcg                                                                <  1:754253/140‑1 (MQ=255)                                                                   aaTAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgcgcgc                                                               >  1:188170/1‑140 (MQ=255)                                                                     tAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCtt                                                             <  2:433192/140‑1 (MQ=255)                                                                            cATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                      <  1:18390/140‑1 (MQ=255)                                                                            cATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                      <  2:188170/140‑1 (MQ=255)                                                                            cATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                      <  2:245741/140‑1 (MQ=255)                                                                            cATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                      <  1:609004/140‑1 (MQ=255)                                                                             aTCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCAc                                                     >  1:659695/1‑140 (MQ=255)                                                                               cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACgg                                                   >  2:752596/1‑140 (MQ=255)                                                                               cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACgg                                                   >  1:388717/1‑140 (MQ=255)                                                                               cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACgg                                                   >  1:350930/1‑140 (MQ=255)                                                                                   tCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCa                                               <  2:77910/140‑1 (MQ=255)                                                                                    cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCttt                                                            <  2:642634/126‑1 (MQ=255)                                                                                    cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCttt                                                            >  1:642634/1‑126 (MQ=255)                                                                                    cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGcc                                                       >  2:939423/1‑131 (MQ=255)                                                                                    cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGcc                                                       <  1:939423/131‑1 (MQ=255)                                                                                      aGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAggg                                            >  2:270117/1‑140 (MQ=255)                                                                                         atatGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                         <  2:388717/140‑1 (MQ=255)                                                                                            tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAag                                      >  1:111489/1‑140 (MQ=255)                                                                                            tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAag                                      >  1:414884/1‑140 (MQ=255)                                                                                                 cGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                         <  2:69059/132‑1 (MQ=255)                                                                                                 cGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                         >  1:69059/1‑132 (MQ=255)                                                                                                 cGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTACTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTg                                 >  2:356122/1‑140 (MQ=255)                                                                                                  gCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                         <  2:828729/131‑1 (MQ=255)                                                                                                  gCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                         >  1:828729/1‑131 (MQ=255)                                                                                                     tAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCAcc                             <  1:349741/140‑1 (MQ=255)                                                                                                       aGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCAt                           <  2:208008/140‑1 (MQ=255)                                                                                                        ggTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATg                          >  1:36015/1‑140 (MQ=255)                                                                                                          ttCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATgcg                        <  2:111489/140‑1 (MQ=255)                                                                                                           tCCCAGTCGGTCGCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATgcgc                       <  1:717138/140‑1 (MQ=255)                                                                                                                  cggtcgCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAAt                >  2:946563/1‑140 (MQ=255)                                                                                                                   ggtcgCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAAtt               >  2:341861/1‑140 (MQ=255)                                                                                                                     tcgCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGt             >  1:196735/1‑140 (MQ=255)                                                                                                                      cgCCATATGGAATACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTc            >  2:575209/1‑140 (MQ=255)                                                                                                                                aaTACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCAtt  <  1:45907/140‑1 (MQ=255)                                                                                                                                aaTACG‑CCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCAtt  >  2:1002531/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATT  >  NC_000913/3102202‑3102469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑