Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,292,420	(T)7→6	intergenic (+55/+89)	galU → / ← hns	UTP‑‑glucose‑1‑phosphate uridylyltransferase/DNA‑binding transcriptional dual regulator H‑NS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,292,414	0	T	.	100.0%	71.9 / NA	18	intergenic (+49/+95)	galU/hns	UTP‑‑glucose‑1‑phosphate uridylyltransferase/DNA‑binding transcriptional dual regulator H‑NS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/10);  total (8/10)

AATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGC  >  NC_000913/1292289‑1292548                                                                                                                              |                                                                                                                                       aaTACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGc                                                                                                                             >  1:689159/1‑136 (MQ=255) aaTACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGc                                                                                                                             <  2:689159/136‑1 (MQ=255)       gTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGcttattcttat                                                                                                                   >  2:394624/1‑140 (MQ=255)           tCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTaaa                                                                                                               <  1:152138/140‑1 (MQ=255)           tCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTaaa                                                                                                               <  1:944264/140‑1 (MQ=255)           tCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTaaa                                                                                                               <  2:167530/140‑1 (MQ=255)                            gCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTaaa                                                                                                               <  1:632473/123‑1 (MQ=255)                            gCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTaaa                                                                                                               >  2:632473/1‑123 (MQ=255)                                            cTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCccgccg                                                                              >  2:750894/1‑140 (MQ=255)                                              ggCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCt                                                                            >  1:765666/1‑140 (MQ=255)                                                cTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTgg                                                                          >  1:5144/1‑140 (MQ=255)                                                 ttGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGc                                                                         <  1:750894/140‑1 (MQ=255)                                                             tGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGc                                                             <  1:394624/140‑1 (MQ=255)                                                             gcggCATTAAGAAGTAACATCCGTATCGGTTTTATCCACGAAACGGCGTTGAGCAATCGACGCC‑TTTTTTTATAGTTTATTTTTATTAAATTGTCTTAAACCGGAAAAAAAAAAATCCCGCCGCTGGCGGGATTTTAAGc                                                             <  2:943636/138‑1 (MQ=255)                                                                                       gggTTTTATCCACGAAACGGCGTTGAGAAATCGACGCC‑TTTTTTTATAGTTTATTCTTATTAAATTGTCTTAAACCGGAAAAAAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGc                                   <  2:5144/139‑1 (MQ=255)                                                                                               tCCACGAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAg                           >  1:320082/1‑140 (MQ=255)                                                                                                    gAAACGGCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAt                      >  2:577080/1‑140 (MQ=255)                                                                                                         ggCGTTGAGCAATCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcgtcg                 <  1:577080/140‑1 (MQ=255)                                                                                                          gCGTTGAGAAATCGACG‑CGTTTTTTTATAGTTTATTCTTATTAATTTGTCTTAACCCGAACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                <  2:621070/140‑1 (MQ=255)                                                                                                                   aaTCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTAc       <  2:320082/140‑1 (MQ=255)                                                                                                                   aaTCGACGCCG‑TTTTTTATAGCTTATTCTTATTAAATTGTCTAAAACCGGAAAAAAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTAc       <  1:973914/140‑1 (MQ=255)                                                                                                                        ccGCCG‑TTTTTTATAGCTTATTCTTATAAAATTGTCTAAAACCGGAAAAAAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGc  <  2:236916/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                       AATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGC  >  NC_000913/1292289‑1292548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑