Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,267,414	Δ1 bp	coding (1170/1467 nt)	yeiQ →	putative dehydrogenase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,267,411	0	G	.	100.0%	168.3 / NA	40	coding (1167/1467 nt)	yeiQ	putative dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/17);  total (23/17)

ATGTCGATTTAACACAATATGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCG  >  NC_000913/2267272‑2267546                                                                                                                                            |                                                                                                                                        aTGTCGATTTAACACAATATGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAg                                                                                                                                         >  1:359993/1‑140 (MQ=255) aTGTCGATTTAACACAATATGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAg                                                                                                                                         >  1:892726/1‑140 (MQ=255)   gTCGATTTAACACAATATGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAggg                                                                                                                                       >  2:44166/1‑140 (MQ=255)   gTCGATTTAACACAATATGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATAGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAggg                                                                                                                                       >  2:212012/1‑140 (MQ=255)     cGATTTAACACAATATGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAgggcg                                                                                                                                     <  1:208258/140‑3 (MQ=255)                aaTATGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACt                                                                                                                         >  2:369176/1‑140 (MQ=255)                   atGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGt                                                                                                                      <  2:769736/140‑1 (MQ=255)                   atGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGt                                                                                                                      <  1:44166/140‑1 (MQ=255)                    tGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGTGAAACGGACTGGTc                                                                                                                     <  1:627757/140‑1 (MQ=255)                     gCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCg                                                                                                                    >  2:413480/1‑140 (MQ=255)                      cGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGt                                                                                                                   >  2:470487/1‑140 (MQ=255)                        gATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTg                                                                                                                 <  1:1159/140‑1 (MQ=255)                        gATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTg                                                                                                                 <  2:915160/140‑1 (MQ=255)                        gATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTg                                                                                                                 <  1:643837/140‑1 (MQ=255)                         aTAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGc                                                                                                                <  2:86455/140‑1 (MQ=255)                          tAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCt                                                                                                               >  2:978739/1‑140 (MQ=255)                            aGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTgg                                                                                                             >  2:995941/1‑140 (MQ=255)                              ttAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCa                                                                                                           >  1:683228/1‑140 (MQ=255)                              ttAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCa                                                                                                           <  1:369176/140‑1 (MQ=255)                                      aCGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGt                                                                                                   >  1:491335/1‑140 (MQ=255)                                             gCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGc                                                                                            <  2:491335/140‑1 (MQ=255)                                                   ccGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATg                                                                                      >  2:713774/1‑140 (MQ=255)                                                     ggCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCg                                                                                    <  2:81751/140‑1 (MQ=255)                                                      gcgcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGt                                                                                   >  1:682817/1‑140 (MQ=255)                                                      gcgcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGt                                                                                   >  2:708670/1‑140 (MQ=255)                                                      gcgcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGt                                                                                   >  1:572756/1‑140 (MQ=255)                                                               cATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgc                                                                          <  1:708670/140‑1 (MQ=255)                                                                         ggCAAATCGCGATGGATGGCAGCCAGAAA‑TACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTtgatga                                                               >  1:444032/1‑140 (MQ=255)                                                                          gCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTtgatga                                                               >  1:563493/1‑140 (MQ=255)                                                                               tCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCg                                                          >  2:631120/1‑140 (MQ=255)                                                                               tCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCg                                                          >  1:454230/1‑140 (MQ=255)                                                                                                cAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGtt                                         <  2:775594/140‑1 (MQ=255)                                                                                                 aGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTc                                        <  2:683228/140‑1 (MQ=255)                                                                                                  gAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATAAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTcg                                       >  2:263098/1‑140 (MQ=255)                                                                                                           gCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCt                              <  2:563493/140‑1 (MQ=255)                                                                                                           gCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCt                              <  2:454230/140‑1 (MQ=255)                                                                                                           gCAACGCATGCTGGCAGGTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCt                              <  2:892726/140‑1 (MQ=255)                                                                                                                            gTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTcgcg             >  1:326514/1‑140 (MQ=255)                                                                                                                            gTATTCGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTcgcg             <  1:73814/140‑1 (MQ=255)                                                                                                                                 cGCATACATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTCGCGAACtt        >  1:888370/1‑140 (MQ=255)                                                                                                                                      aCATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGc   >  2:550948/1‑140 (MQ=255)                                                                                                                                      aCATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGc   >  2:179600/1‑140 (MQ=255)                                                                                                                                       cATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCg  >  2:168920/1‑140 (MQ=255)                                                                                                                                       cATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCg  >  1:1020229/1‑140 (MQ=255)                                                                                                                                       cATCA‑GGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCg  >  2:956049/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                        ATGTCGATTTAACACAATATGCGGATAAGTTAATTGCACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATTGATGTTCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCG  >  NC_000913/2267272‑2267546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑