Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,004,160	(G)7→8	coding (214/711 nt)	ycbF →	putative fimbrial chaperone YcbF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,004,153	1	.	G	100.0%	177.0 / NA	50	A69A (GCC→GCG)	ycbF	putative fimbrial chaperone YcbF
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/24);  total (26/24)

AATTGCAGGCAGGAGTGGTGGTCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGAAACGC  >  NC_000913/1004014‑1004286                                                                                                                                             |                                                                                                                                      aaTTGCAGGCAGGAGTGGTGGTCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGcc                                                                                                                                        >  1:341328/1‑140 (MQ=255)                  gtggtCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGa                                                                                                                       >  2:338544/1‑140 (MQ=255)                   tggtCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGAc                                                                                                                      >  1:751504/1‑140 (MQ=255)                         gcggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGcc                                                                                                                <  2:198776/140‑1 (MQ=255)                                          tttCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCg                                                                                               <  1:854581/140‑1 (MQ=255)                                              cGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                                                                           <  2:751504/140‑1 (MQ=255)                                                 cagacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc                                                                                        >  1:237886/1‑140 (MQ=255)                                                 cagacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc                                                                                        <  1:334540/140‑1 (MQ=255)                                                 cagacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACAATTACTGGCCGCTCCGcc                                                                                        <  1:696973/140‑1 (MQ=255)                                                              gATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTg                                                                           >  1:619633/1‑140 (MQ=255)                                                                  tCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAg                                                                        <  2:817578/139‑1 (MQ=255)                                                                  tCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAg                                                                        >  1:817578/1‑139 (MQ=255)                                                                   cTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGcc                                                                      <  1:338544/140‑1 (MQ=255)                                                                              aCTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTAGTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAg                                                           >  2:951564/1‑140 (MQ=255)                                                                                tAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCgg                                                                   <  1:38013/130‑1 (MQ=255)                                                                                tAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCgg                                                                   >  2:38013/1‑130 (MQ=255)                                                                                  aTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCAc                                                       >  2:339135/1‑140 (MQ=255)                                                                                   tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCgg                                                                   <  2:445200/127‑1 (MQ=255)                                                                                   tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCgg                                                                   >  1:445200/1‑127 (MQ=255)                                                                                   tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGt                                                                  <  1:342985/128‑1 (MQ=255)                                                                                   tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGt                                                                  >  2:342985/1‑128 (MQ=255)                                                                                   tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACg                                                      >  1:810982/1‑140 (MQ=255)                                                                                    aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGt                                                     <  2:237886/140‑1 (MQ=255)                                                                                        gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcg                                                 >  2:519600/1‑140 (MQ=255)                                                                                        gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcg                                                 <  1:115267/140‑1 (MQ=255)                                                                                        gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcg                                                 >  2:931855/1‑140 (MQ=255)                                                                                        gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcg                                                 >  1:85200/1‑140 (MQ=255)                                                                                        gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcg                                                 >  2:197828/1‑140 (MQ=255)                                                                                        gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcg                                                 >  2:115267/1‑140 (MQ=255)                                                                                        gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcg                                                 >  2:658470/1‑140 (MQ=255)                                                                                            ggAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTACCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTg                                             >  2:128366/1‑140 (MQ=255)                                                                                             gAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAATGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGc                                            >  2:590765/1‑140 (MQ=255)                                                                                                  cTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTgaga                                       >  2:200570/1‑140 (MQ=255)                                                                                                       ttattaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGa                                  >  1:423403/1‑140 (MQ=255)                                                                                                       ttattaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGa                                  >  1:422919/1‑140 (MQ=255)                                                                                                          ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAg                               >  2:876366/1‑140 (MQ=255)                                                                                                           taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGc                              <  1:243569/140‑1 (MQ=255)                                                                                                               aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACa                          >  2:241391/1‑140 (MQ=255)                                                                                                               aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACa                          >  2:446146/1‑140 (MQ=255)                                                                                                                gTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACAt                         <  1:153793/140‑1 (MQ=255)                                                                                                                gTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACAt                         <  1:241391/140‑1 (MQ=255)                                                                                                                gTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACAt                         <  1:210561/140‑1 (MQ=255)                                                                                                                  aaaaTCAACCGCCCAACGCGTTGGGCCGGGGGGGGGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCt                       <  2:619633/140‑1 (MQ=255)                                                                                                                  aaaaTCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCt                       <  1:876366/140‑1 (MQ=255)                                                                                                                     aTCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGc                    <  1:446146/140‑1 (MQ=255)                                                                                                                      tCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGcc                   <  2:85200/140‑1 (MQ=255)                                                                                                                       cAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCt                  <  1:339135/140‑1 (MQ=255)                                                                                                                          ccGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCtgtg               <  2:341328/140‑1 (MQ=255)                                                                                                                                    cgTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGaaa     <  1:931855/140‑1 (MQ=255)                                                                                                                                     gTTGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGAAAc    <  1:312880/140‑1 (MQ=255)                                                                                                                                       tGGGCCGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGAAACGc  >  2:230586/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                      AATTGCAGGCAGGAGTGGTGGTCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGAAACGC  >  NC_000913/1004014‑1004286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑