| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,351,196 | (G)7→8 | intergenic (‑157/+211) | fabI ← / ← ycjD | enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,351,189 | 1 | . | G | 94.1% | 168.4 / 3.4 | 51 | intergenic (‑150/+218) | fabI/ycjD | enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (22/26); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
TGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA > NC_000913/1351058‑1351319 | tgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑gggggggg < 2:398208/140‑2 (MQ=255) cTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTACGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCtt < 1:625632/140‑1 (MQ=255) tgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCt < 2:259419/140‑1 (MQ=255) aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCt < 1:336052/140‑1 (MQ=255) aGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTa < 1:680733/135‑1 (MQ=255) aGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTa > 2:680733/1‑135 (MQ=255) tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCC‑CTCCCCag > 1:718209/1‑140 (MQ=255) cgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg < 1:412531/140‑1 (MQ=255) tAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGTGCGa < 2:341815/140‑1 (MQ=255) cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa < 1:701555/133‑1 (MQ=255) cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa > 2:701555/1‑133 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa > 1:90060/1‑140 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa > 2:550068/1‑140 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa > 2:142268/1‑140 (MQ=255) aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt < 1:550068/140‑1 (MQ=255) cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGc < 2:295329/140‑1 (MQ=255) aGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCC‑CTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaa < 2:718209/140‑1 (MQ=255) gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaa < 2:90060/140‑1 (MQ=255) gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa > 2:117073/1‑126 (MQ=255) gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa < 1:117073/126‑1 (MQ=255) aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGTaaa > 1:362464/1‑140 (MQ=255) tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAat > 1:568084/1‑140 (MQ=255) tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAat > 2:861602/1‑140 (MQ=255) ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTa > 1:552013/1‑140 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAcc > 1:127922/1‑140 (MQ=255) aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCccacca > 2:696960/1‑140 (MQ=255) tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaa < 1:861602/140‑1 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa > 1:898775/1‑140 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa > 2:96155/1‑140 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa < 1:86428/140‑1 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa < 2:245925/140‑1 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa > 1:245925/1‑140 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa < 1:52836/140‑1 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa > 2:651362/1‑140 (MQ=255) cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaac > 2:877897/1‑140 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg > 2:305397/1‑140 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg > 2:846155/1‑140 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGACTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa < 1:873599/136‑1 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGACTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa > 2:873599/1‑136 (MQ=255) cTGTGAGTAGCTATAGTTGCCAGGTCCGGCCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGc < 2:930479/140‑1 (MQ=255) gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATAc < 1:877897/140‑1 (MQ=255) cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCCCCCCCTAACCCTCCCCCCAGAGGGGCGGGGGGGCCGTATTGTGCAAATATTGTTACCCCAGCAACAAAAAGGCCCATACAGcc > 1:46485/1‑140 (MQ=255) tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGccc < 1:96155/140‑1 (MQ=255) gTTGCCCGGGCCGGCCGGGGCAGGCTGGGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa < 2:46485/140‑1 (MQ=37) gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa < 1:651362/140‑1 (MQ=255) gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa > 2:386793/1‑140 (MQ=255) aCCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg < 1:386793/140‑1 (MQ=255) aCCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg < 1:142268/140‑1 (MQ=255) aCCGGAGCAGGCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCCCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGGCCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGATCATACAGCCCCCAACCCTTTCATGGCg > 1:666103/1‑140 (MQ=38) ccGGAGCAGGCTGCGGCAGGGGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg < 1:636066/140‑1 (MQ=255) ccGGAGCAGGCTGCGGCAGGGGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg < 1:745504/140‑1 (MQ=255) gCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa < 2:127922/140‑1 (MQ=255) gCTGCGGCAG‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCCCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa < 2:898775/140‑1 (MQ=255) | TGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA > NC_000913/1351058‑1351319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |