Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,977,215	(A)8→9	intergenic (‑76/+51)	motA ← / ← flhC	motility protein A/DNA‑binding transcriptional dual regulator FlhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,977,207	1	.	A	100.0%	240.6 / NA	68	intergenic (‑68/+59)	motA/flhC	motility protein A/DNA‑binding transcriptional dual regulator FlhC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (34/34);  total (34/34)

GGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCT  >  NC_000913/1977077‑1977340                                                                                                                                    |                                                                                                                                       ggTAATAAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑‑aaaaaaaaa                                                                                                                              <  2:778356/140‑2 (MQ=255)    catcaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑‑aaaaaaaaagca                                                                                                                           >  1:935574/1‑136 (MQ=255)    catcaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑‑aaaaaaaaagca                                                                                                                           <  1:399928/140‑5 (MQ=255)     atcaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑‑aaaaaaaaagcag                                                                                                                          >  2:716947/1‑135 (MQ=255)      tcaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAagcagc                                                                                                                          >  1:308789/1‑140 (MQ=255)        aaaTAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCgg                                                                                                                        >  2:620318/1‑140 (MQ=255)            aaCCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAAAGCAGCGGTAc                                                                                                                     <  2:481975/140‑1 (MQ=255)             aCCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTAcgt                                                                                                                   >  1:441033/1‑140 (MQ=255)                 ccgAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGtt                                                                                                               >  2:641004/1‑140 (MQ=255)                 ccgAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGtt                                                                                                               >  2:844340/1‑140 (MQ=255)                   gAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTAc                                                                                                             <  2:441033/140‑1 (MQ=255)                   gAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTAc                                                                                                             <  2:177327/140‑1 (MQ=255)                     aaaCTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCg                                                                                                           <  1:593438/140‑1 (MQ=255)                      aaCTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCgc                                                                                                          <  1:844340/140‑1 (MQ=255)                           tACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGctgctg                                                                                                     <  2:97245/140‑1 (MQ=255)                           tACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGctgctg                                                                                                     >  2:570779/1‑140 (MQ=255)                            aCCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTgg                                                                                                    >  2:593279/1‑140 (MQ=255)                                agaACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATg                                                                                                >  1:45602/1‑140 (MQ=255)                                agaACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATg                                                                                                >  2:107793/1‑140 (MQ=255)                                     aacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcg                                                                                           >  2:190202/1‑140 (MQ=255)                                     aacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcg                                                                                           <  1:107793/140‑1 (MQ=255)                                     aacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcg                                                                                           <  2:308789/140‑1 (MQ=255)                                      acCAGGTACCCTAATAAGATAAGCCCGACATCACCCTTCCACTGTTGACCATAAAAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGCCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcgc                                                                                          <  2:935574/140‑1 (MQ=255)                                      acCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcgc                                                                                          <  1:761948/140‑1 (MQ=255)                                       ccAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcg                                                                                           >  1:209733/1‑138 (MQ=255)                                       ccAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcg                                                                                           <  2:209733/138‑1 (MQ=255)                                            tAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCAcc                                                                                    >  1:844896/1‑140 (MQ=255)                                              aCCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGt                                                                                  <  1:641004/140‑1 (MQ=255)                                               ccTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCt                                                                                        <  1:617759/133‑1 (MQ=255)                                               ccTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCt                                                                                        >  2:190893/1‑133 (MQ=255)                                               ccTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCt                                                                                        <  1:190893/133‑1 (MQ=255)                                               ccTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCt                                                                                        >  2:617759/1‑133 (MQ=255)                                                  aataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCa                                                                              <  1:190202/140‑1 (MQ=255)                                                    taagataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGt                                                                            >  1:47604/1‑140 (MQ=255)                                                    taagataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGt                                                                            >  1:360137/1‑140 (MQ=255)                                                     aagataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGtt                                                                           >  2:875435/1‑140 (MQ=255)                                                        ataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaaa                                                                        >  1:257659/1‑140 (MQ=255)                                                         taagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAAc                                                                       >  1:1079/1‑140 (MQ=255)                                                         taagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAAc                                                                       <  2:567707/140‑1 (MQ=255)                                                         taagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAAc                                                                       <  1:875435/140‑1 (MQ=255)                                                          aagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGt                                                                                  <  1:860229/128‑1 (MQ=255)                                                          aagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGt                                                                                  >  2:860229/1‑128 (MQ=255)                                                          aagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaaa                                                                        <  2:183965/138‑1 (MQ=255)                                                          aagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaaa                                                                        >  1:183965/1‑138 (MQ=255)                                                          aagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACa                                                                      >  2:513508/1‑140 (MQ=255)                                                            gCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGc                                                                    <  2:737949/140‑1 (MQ=255)                                                               cGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTg                                                                 >  2:195480/1‑140 (MQ=255)                                                                  catcatCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTAc                                                              >  1:802546/1‑140 (MQ=255)                                                                    tcatcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActc                                                            <  1:620318/140‑1 (MQ=255)                                                                     catcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActct                                                           >  2:126269/1‑140 (MQ=255)                                                                            ccACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCa                                                    <  2:45602/140‑1 (MQ=255)                                                                            ccACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCa                                                    >  1:489197/1‑140 (MQ=255)                                                                            ccACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCa                                                    <  1:793405/140‑1 (MQ=255)                                                                                 gTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcag                                               <  2:991237/140‑1 (MQ=255)                                                                                  ttGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagc                                              >  2:812040/1‑140 (MQ=255)                                                                                       cATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagcag                                            <  2:897289/137‑1 (MQ=255)                                                                                       cATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagcag                                            >  1:897289/1‑137 (MQ=255)                                                                                        aTGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTtgt                                        <  2:257659/140‑1 (MQ=255)                                                                                         tGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTtgtg                                       <  1:570779/140‑1 (MQ=255)                                                                                               gATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCTTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGataa                                 >  2:911762/1‑140 (MQ=255)                                                                                               gATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGtt                                          >  1:239356/1‑131 (MQ=255)                                                                                               gATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGtt                                          <  2:239356/131‑1 (MQ=255)                                                                                                       gtcgtcAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCa                         <  1:812040/140‑1 (MQ=255)                                                                                                       gtcgtcAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCa                         >  1:867346/1‑140 (MQ=255)                                                                                                        tcgtcAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAg                        >  2:649085/1‑140 (MQ=255)                                                                                                        tcgtcAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAg                        <  2:47604/140‑1 (MQ=255)                                                                                                          gtcAGGCGTTAACGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGa                      <  1:513508/140‑1 (MQ=255)                                                                                                                     aCGCGCGATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAg           >  2:608589/1‑140 (MQ=255)                                                                                                                       gcgcgATTGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGtt         <  1:195480/140‑1 (MQ=255)                                                                                                                             ttGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTa       >  2:241556/1‑136 (MQ=255)                                                                                                                             ttGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTa       <  1:241556/136‑1 (MQ=255)                                                                                                                              tGGGGCA‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCt  <  2:1079/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       GGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑‑AAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCT  >  NC_000913/1977077‑1977340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑