Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	101,492	T→C	V243A (GTG→GCG)	murC →	UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	101,492	0	T	C	100.0%	134.0 / NA	38	V243A (GTG→GCG)	murC	UDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/16);  total (22/16)

ACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCG  >  NC_000913/101357‑101628                                                                                                                                        |                                                                                                                                         aCCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCgggg                                                                                                                                      >  1:828730/1‑140 (MQ=255) aCCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTAATCCGCGAATTGTTACCGCGAGCgggg                                                                                                                                      >  2:602472/1‑140 (MQ=255)             cctaccAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACg                                                                                                                          >  2:618065/1‑140 (MQ=255)                  cAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTa                                                                                                                     >  1:583813/1‑140 (MQ=255)                     ggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGt                                                                                                                                   >  2:801653/1‑123 (MQ=255)                     ggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGt                                                                                                                                   <  1:801653/123‑1 (MQ=255)                         aCTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTc                                                                                                              >  1:534892/1‑140 (MQ=255)                          cTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCa                                                                                                             >  2:135859/1‑140 (MQ=255)                              gagaATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGa                                                                                                         <  2:534892/140‑1 (MQ=255)                                          cAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAcgt                                                                                             <  2:584678/140‑1 (MQ=255)                                            gACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgc                                                                                           <  2:828730/140‑1 (MQ=255)                                             aCTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgcg                                                                                          >  1:546045/1‑140 (MQ=255)                                                   attaATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTaga                                                                                    <  1:135859/140‑1 (MQ=255)                                                            cTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATca                                                                           <  1:152672/140‑1 (MQ=255)                                                                     cTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTgg                                                                  <  2:583813/140‑1 (MQ=255)                                                                       gCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGcc                                                                >  1:264281/1‑140 (MQ=255)                                                                         cGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCg                                                              <  1:1006253/140‑1 (MQ=255)                                                                                 ggTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCa                                                      <  1:285816/140‑1 (MQ=255)                                                                                 ggTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCa                                                      <  1:618065/140‑1 (MQ=255)                                                                                     gTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACttt                                                  >  1:756240/1‑140 (MQ=255)                                                                                             aTGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgct                                          >  1:866016/1‑140 (MQ=255)                                                                                                     tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTa                                                 >  1:586093/1‑125 (MQ=255)                                                                                                     tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTa                                                 <  2:586093/125‑1 (MQ=255)                                                                                                     tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTgcg                                       <  2:499899/135‑1 (MQ=255)                                                                                                     tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTgcg                                       >  1:499899/1‑135 (MQ=255)                                                                                                       atgatCCGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGAc                                >  2:987121/1‑140 (MQ=255)                                                                                                            ccGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAaga                           >  2:219371/1‑140 (MQ=255)                                                                                                            ccGGTGATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAaga                           >  2:1013069/1‑140 (MQ=255)                                                                                                                 gATCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGa                      >  2:230573/1‑140 (MQ=255)                                                                                                                  aTCCGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGAt                     <  1:266913/140‑1 (MQ=255)                                                                                                                    ccGCGAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATgc                   >  2:439253/1‑140 (MQ=255)                                                                                                                     cgcgAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATgcg                  >  1:368290/1‑140 (MQ=255)                                                                                                                       cgAATTGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGcgcg                >  2:647026/1‑140 (MQ=255)                                                                                                                            tGTTACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCAcc           <  2:546045/140‑1 (MQ=255)                                                                                                                              ttACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGAt                     >  2:241687/1‑128 (MQ=255)                                                                                                                              ttACCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGAt                     <  1:241687/128‑1 (MQ=255)                                                                                                                                aCCGCGAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGa       <  2:866016/140‑1 (MQ=255)                                                                                                                                    cgAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATgc   >  1:990373/1‑140 (MQ=255)                                                                                                                                     gAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATgcg  <  2:368290/140‑1 (MQ=255)                                                                                                                                     gAGCGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATgcg  >  2:837722/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         ACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCG  >  NC_000913/101357‑101628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑