Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	508,296	A→G	I27V (ATC→GTC)	ybaQ →	putative DNA‑binding transcriptional regulator YbaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	508,296	0	A	G	100.0%	296.8 / NA	83	I27V (ATC→GTC)	ybaQ	putative DNA‑binding transcriptional regulator YbaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/40);  total (43/40)

TTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTA  >  NC_000913/508159‑508430                                                                                                                                          |                                                                                                                                       tttGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTc                                                                                                                                      >  2:231408/1‑140 (MQ=255)  ttGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCa                                                                                                                                     >  2:488357/1‑140 (MQ=255)  ttGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCa                                                                                                                                     <  1:382566/140‑1 (MQ=255)   tGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCaa                                                                                                                                    <  1:120741/140‑1 (MQ=255)     tATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATg                                                                                                                                  <  1:856229/140‑1 (MQ=255)         aTTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGa                                                                                                                                 >  2:289047/1‑137 (MQ=255)         aTTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGa                                                                                                                                 <  1:289047/137‑1 (MQ=255)         aTTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGtt                                                                                                                              >  1:458106/1‑140 (MQ=255)           tCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAg                                                                                                                            >  2:78047/1‑140 (MQ=255)               tATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCa                                                                                                                          <  1:84062/138‑1 (MQ=255)               tATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCa                                                                                                                          >  2:84062/1‑138 (MQ=255)                   ttCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTtg                                                                                                                    <  1:78047/140‑1 (MQ=255)                    tCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTtgc                                                                                                                   >  2:491141/1‑140 (MQ=255)                    tCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTtgc                                                                                                                   >  1:224334/1‑140 (MQ=255)                    tCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTtgc                                                                                                                   >  2:915280/1‑140 (MQ=255)                     cTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTtgct                                                                                                                  >  1:627468/1‑140 (MQ=255)                      ttGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTtgctg                                                                                                                 >  1:633559/1‑140 (MQ=255)                       tGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATg                                                                                                                                  <  1:823549/122‑1 (MQ=255)                       tGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATg                                                                                                                                  >  2:823549/1‑122 (MQ=255)                         cATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCAt                                                                                                              >  2:17608/1‑140 (MQ=255)                         cATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCAt                                                                                                              >  1:169582/1‑140 (MQ=255)                         cATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCAt                                                                                                              >  2:1006375/1‑140 (MQ=255)                         cATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCAt                                                                                                              >  2:825/1‑140 (MQ=255)                          aTCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATg                                                                                                             <  2:169582/140‑1 (MQ=255)                            cGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGtt                                                                                                           <  2:633559/140‑1 (MQ=255)                             gCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCa                                                                                                                          <  2:691887/124‑1 (MQ=255)                             gCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCa                                                                                                                          >  1:691887/1‑124 (MQ=255)                             gCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTc                                                                                                          >  1:639755/1‑140 (MQ=255)                              cTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATg                                                                                                             <  1:677301/136‑1 (MQ=255)                              cTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATg                                                                                                             >  2:677301/1‑136 (MQ=255)                              cTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCa                                                                                                         >  1:833724/1‑140 (MQ=255)                              cTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCa                                                                                                         >  1:961213/1‑140 (MQ=255)                              cTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCa                                                                                                         >  2:220148/1‑140 (MQ=255)                              cTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCa                                                                                                         >  2:892438/1‑140 (MQ=255)                              cTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGCTGCTGCATGTTCa                                                                                                         >  1:146033/1‑140 (MQ=255)                                      aaGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATa                                                                                                 >  1:600597/1‑140 (MQ=255)                                       aGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAg                                                                                                <  2:170110/140‑1 (MQ=255)                                       aGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAg                                                                                                <  2:982259/140‑1 (MQ=255)                                        ggAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGtt                                                                                                           <  1:44848/128‑1 (MQ=255)                                        ggAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGtt                                                                                                           >  2:44848/1‑128 (MQ=255)                                          aaGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGt                                                                                             <  1:825/140‑1 (MQ=255)                                           aGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTc                                                                                            >  1:395057/1‑140 (MQ=255)                                            gCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCa                                                                                           <  2:961213/140‑1 (MQ=255)                                            gCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCa                                                                                           <  1:347403/140‑1 (MQ=255)                                            gCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCa                                                                                           <  2:314488/140‑1 (MQ=255)                                                 aGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCa                                                                                      <  2:600597/140‑1 (MQ=255)                                                 aGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCa                                                                                      <  2:458106/140‑1 (MQ=255)                                                 aGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCa                                                                                      <  2:224334/140‑1 (MQ=255)                                                 aGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCa                                                                                      <  1:491141/140‑1 (MQ=255)                                                  gTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACGCCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCAc                                                                                     <  2:146033/140‑1 (MQ=255)                                                  gTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCAc                                                                                     <  1:915280/140‑1 (MQ=255)                                                  gTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCAc                                                                                     <  2:627468/140‑1 (MQ=255)                                                  gTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCAGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCAc                                                                                     <  2:950175/140‑1 (MQ=255)                                                      aaaaCCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGAt                                                                                 >  1:197984/1‑140 (MQ=255)                                                          ccATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAAt                                                                             <  1:220148/140‑1 (MQ=255)                                                            aTGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATaa                                                                           <  1:231408/140‑1 (MQ=255)                                                            aTGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATaa                                                                           <  2:218/140‑1 (MQ=255)                                                                 aCAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATc                                                                      <  2:833724/140‑1 (MQ=255)                                                                   aGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGt                                                                    <  2:112580/140‑1 (MQ=255)                                                                    ggCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTa                                                                   >  1:204704/1‑140 (MQ=255)                                                                       aacaaGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAAc                                                                <  1:483561/140‑1 (MQ=255)                                                                             aaaaCCGACGACACCCGGAGATATTCTTCTTTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCacta                                                          <  1:881603/140‑1 (MQ=255)                                                                               aaCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCactact                                                        >  1:925504/1‑140 (MQ=255)                                                                                    acgacCCCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGAt                                                   <  2:508976/140‑1 (MQ=255)                                                                                    acgacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACt                                                               <  2:213082/128‑1 (MQ=255)                                                                                    acgacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACt                                                               >  1:213082/1‑128 (MQ=255)                                                                                    acgacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGAt                                                   <  2:395057/140‑1 (MQ=255)                                                                                                   atTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGc                                    >  1:410280/1‑140 (MQ=255)                                                                                                        tctcTATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAg                               <  2:204704/140‑1 (MQ=255)                                                                                                         ctctATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGa                                  >  2:965947/1‑136 (MQ=255)                                                                                                         ctctATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGa                                  <  1:965947/136‑1 (MQ=255)                                                                                                            tATGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGtttt                           >  1:292423/1‑140 (MQ=255)                                                                                                              tGAATATCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTg                         >  1:826591/1‑140 (MQ=255)                                                                                                                   atCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATAcc                    >  1:382826/1‑140 (MQ=255)                                                                                                                    tCTGGAACCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACca                   <  2:382826/140‑1 (MQ=255)                                                                                                                          aCCGCTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCg             >  1:624751/1‑140 (MQ=255)                                                                                                                             gCTTGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAtt          >  1:561523/1‑140 (MQ=255)                                                                                                                              cTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACGGTCGAttt         >  1:888078/1‑140 (MQ=255)                                                                                                                              cTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAttt         >  2:703287/1‑140 (MQ=255)                                                                                                                              cTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAttt         >  1:40469/1‑140 (MQ=255)                                                                                                                              cTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAttt         >  2:605220/1‑140 (MQ=255)                                                                                                                              cTCGATTTGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGGTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAttt         >  1:946413/1‑140 (MQ=255)                                                                                                                                     tGAAAGTCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTa  <  1:479622/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TTTGTATGATTCAGTATGTTCTTGCATCGCTATTCACAAGGAAGCAACAGTTAAAAACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTA  >  NC_000913/508159‑508430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑