Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,736,296	G→A	A51T (GCA→ACA)	bamD →	outer membrane protein assembly factor BamD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,736,296	0	G	A	100.0%	190.0 / NA	54	A51T (GCA→ACA)	bamD	outer membrane protein assembly factor BamD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/30);  total (24/30)

TCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAT  >  NC_000913/2736163‑2736432                                                                                                                                      |                                                                                                                                         tCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGGCTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATaa                                                                                                                                    >  1:276300/1‑140 (MQ=255)  cTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAAc                                                                                                                                   <  2:432057/140‑1 (MQ=255)    ggtggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGc                                                                                                                                 <  2:835586/140‑1 (MQ=255)         cAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTg                                                                                                                            <  1:197071/140‑1 (MQ=255)          aGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTgg                                                                                                                           <  2:52531/140‑1 (MQ=255)          aGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTgg                                                                                                                           <  1:381231/140‑1 (MQ=255)           gccgccACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGa                                                                                                                          >  1:902175/1‑140 (MQ=255)              gccACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGc                                                                                                                       <  2:902175/140‑1 (MQ=255)               ccACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCg                                                                                                                      <  1:423682/140‑1 (MQ=255)                         ccTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATc                                                                                                            >  1:4443/1‑140 (MQ=255)                                  ggCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGGCAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGt                                                                                                   >  2:945293/1‑140 (MQ=255)                                   gCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGtt                                                                                                  <  1:384768/140‑1 (MQ=255)                                   gCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGtt                                                                                                  <  1:945514/140‑1 (MQ=255)                                       gTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGt                                                                                              >  1:247263/1‑140 (MQ=255)                                               gggTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTc                                                                                      <  1:719963/140‑1 (MQ=255)                                                  tCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgca                                                                                   >  2:873352/1‑140 (MQ=255)                                                  tCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgca                                                                                   <  1:777744/140‑1 (MQ=255)                                                     aaGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagca                                                                                >  2:828661/1‑140 (MQ=255)                                                     aaGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagca                                                                                >  1:673069/1‑140 (MQ=255)                                                       ggAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAgg                                                                              <  1:873352/140‑1 (MQ=255)                                                       ggAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAgg                                                                              <  2:32352/140‑1 (MQ=255)                                                         aagaagTACCTGATAAACCGTCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAAGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTg                                                                            <  2:276300/140‑1 (MQ=255)                                                          agaagTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGc                                                                           >  2:456019/1‑140 (MQ=255)                                                                  cTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                                                                        >  2:582551/1‑135 (MQ=255)                                                                  cTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                                                                        <  1:582551/135‑1 (MQ=255)                                                                   tGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtc                                                                  >  2:551448/1‑140 (MQ=255)                                                                   tGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtc                                                                  <  2:4443/140‑1 (MQ=255)                                                                      tAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCa                                                               >  2:207437/1‑140 (MQ=255)                                                                      tAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCa                                                               <  2:247263/140‑1 (MQ=255)                                                                      tAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCa                                                               >  2:706477/1‑140 (MQ=255)                                                                         tCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtct                                                                 <  2:974712/135‑1 (MQ=255)                                                                         tCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtct                                                                 >  1:974712/1‑135 (MQ=255)                                                                         tCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCt                                                            >  1:168410/1‑140 (MQ=255)                                                                           cGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                                                                        >  1:253061/1‑126 (MQ=255)                                                                           cGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                                                                        <  2:253061/126‑1 (MQ=255)                                                                             ccAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGc                                                        <  2:381432/140‑1 (MQ=255)                                                                              cAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGcc                                                       <  2:673069/140‑1 (MQ=255)                                                                              cAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGcc                                                       <  1:393348/140‑1 (MQ=255)                                                                              cAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGcc                                                       <  1:551448/140‑1 (MQ=255)                                                                                               aCTGCACTACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGa                                      >  2:341867/1‑140 (MQ=255)                                                                                                cTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGAt                                     <  1:259889/140‑1 (MQ=255)                                                                                                 tGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGAtt                                    <  1:706477/140‑1 (MQ=255)                                                                                                  gCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGACCTCATCTACGCCTACTATAAAAACGCCGAttt                                   <  2:843999/140‑1 (MQ=255)                                                                                                           aaGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGc                          >  1:923243/1‑140 (MQ=255)                                                                                                            aGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGca                         <  1:828661/140‑1 (MQ=255)                                                                                                                gCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAgg                     >  2:884785/1‑140 (MQ=255)                                                                                                                  aGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCt                   >  2:698855/1‑140 (MQ=255)                                                                                                                    gACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGc                 <  1:25893/140‑1 (MQ=255)                                                                                                                       ggTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCat              >  2:148435/1‑140 (MQ=255)                                                                                                                                  aCAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTa   >  1:920324/1‑140 (MQ=255)                                                                                                                                   cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCatcgat          <  2:765497/132‑1 (MQ=255)                                                                                                                                   cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAt  <  1:772704/140‑1 (MQ=255)                                                                                                                                   cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAt  >  1:118853/1‑140 (MQ=255)                                                                                                                                   cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAt  <  1:341867/140‑1 (MQ=255)                                                                                                                                   cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAt  >  2:24437/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         TCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAT  >  NC_000913/2736163‑2736432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑