Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,957	T→C	E685E (GAA→GAG)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,957	0	T	C	100.0%	159.7 / NA	46	E685E (GAA→GAG)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/26);  total (20/26)

AGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGC  >  NC_000913/2819825‑2820091                                                                                                                                     |                                                                                                                                       aGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAgaga                                                                                                                                 <  1:153988/140‑1 (MQ=255) aGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAgaga                                                                                                                                 <  2:377168/140‑1 (MQ=255)   ccAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAgaga                                                                                                                                 <  2:762853/138‑1 (MQ=255)   ccAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAgaga                                                                                                                                 >  1:762853/1‑138 (MQ=255)        ttattGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCg                                                                                                                          <  1:173247/140‑1 (MQ=255)          attGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGa                                                                                                                        <  1:165674/140‑1 (MQ=255)           ttGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGaa                                                                                                                       <  2:21872/140‑1 (MQ=255)           ttGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGaa                                                                                                                       <  2:266294/140‑1 (MQ=255)           ttGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGaa                                                                                                                       <  1:772459/140‑1 (MQ=255)               tATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGa                                                                                                                   <  2:316283/140‑1 (MQ=255)               tATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGa                                                                                                                   <  1:698267/140‑1 (MQ=255)                aTCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGAc                                                                                                                  >  2:180741/1‑140 (MQ=255)                 tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGTACAGAcc                                                                                                                 >  2:669310/1‑140 (MQ=255)                 tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGTACAGAcc                                                                                                                 <  1:669310/140‑1 (MQ=255)                 tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGAcc                                                                                                                 >  1:10585/1‑140 (MQ=255)                 tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGAcc                                                                                                                 >  1:139996/1‑140 (MQ=255)                         cagcagATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCAc                                                                                                         <  1:180741/140‑1 (MQ=255)                            cagATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGa                                                                                                                   <  1:716457/127‑1 (MQ=255)                            cagATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGa                                                                                                                   >  2:716457/1‑127 (MQ=255)                              gATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTg                                                                                                    <  2:462510/140‑1 (MQ=255)                               aTGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGc                                                                                                   >  1:181253/1‑140 (MQ=255)                               aTGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGc                                                                                                   >  2:296324/1‑140 (MQ=255)                                 gcgcGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCgg                                                                                                 <  2:498650/140‑1 (MQ=255)                                 gcgcGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCgg                                                                                                 <  1:240854/140‑1 (MQ=255)                                   gcgACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCt                                                                                               >  2:344231/1‑140 (MQ=255)                                           tcTTAAGCGATCGCGGGCTGCATGAACGGTGGGGATAGCGCCTTCTCCGTTTCCCGCTTCGATCCGACGAACGCCGGCAGCAGTCCCCGACTCAGAGATGATGCGGATCAGACCAATATCACCAGTGCGGCTGGCGTGAg                                                                                       <  2:924826/139‑1 (MQ=255)                                                        cTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACaa                                                                          <  1:592480/140‑1 (MQ=255)                                                          gTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACt                                                                        <  1:296324/140‑1 (MQ=255)                                                          gTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCATGAGTACCGCCACACAACt                                                                        <  1:932122/140‑1 (MQ=255)                                                                    aCGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGaa                                                              <  1:242606/140‑1 (MQ=255)                                                                          gCGATAGCCCCTTCCCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGcc                                                        <  2:247716/140‑1 (MQ=255)                                                                                      tctcCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCAc                                            >  1:303910/1‑140 (MQ=255)                                                                                           ggTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTa                                       >  2:867759/1‑140 (MQ=255)                                                                                            gTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGcc                                                        <  2:311619/122‑1 (MQ=255)                                                                                            gTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGcc                                                        >  1:311619/1‑122 (MQ=255)                                                                                              tACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTAcgc                                    >  2:384434/1‑140 (MQ=255)                                                                                               aCCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTAcgcg                                   <  2:10585/140‑1 (MQ=255)                                                                                                 cGCTTCGATACGACGAACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCt                                 >  2:28631/1‑140 (MQ=255)                                                                                                              cgaACGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTtctc                    >  2:784309/1‑140 (MQ=255)                                                                                                                aaCGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGc                  >  1:908196/1‑140 (MQ=255)                                                                                                                  cGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGc                  <  2:26469/138‑1 (MQ=255)                                                                                                                  cGCCTGCAGCAGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGc                  >  1:26469/1‑138 (MQ=255)                                                                                                                       gcagcaGTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAg           <  2:302631/140‑1 (MQ=255)                                                                                                                        cagcagTACCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAgc          <  1:784309/140‑1 (MQ=255)                                                                                                                               aCCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCg   >  2:558213/1‑140 (MQ=255)                                                                                                                               aCCCGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCg   >  1:1012135/1‑140 (MQ=255)                                                                                                                                cccGACTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGc  >  1:907988/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       AGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGC  >  NC_000913/2819825‑2820091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑