Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,873,008	T→C	intergenic (‑188/+6)	ftsB ← / ← ygbE	cell division protein FtsB/conserved inner membrane protein YgbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,873,008	0	T	C	100.0%	329.6 / NA	92	intergenic (‑188/+6)	ftsB/ygbE	cell division protein FtsB/conserved inner membrane protein YgbE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/45);  total (47/45)

TCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACCGCGA  >  NC_000913/2872878‑2873138                                                                                                                                   |                                                                                                                                   tctGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAg                                                                                                                           >  2:544105/1‑140 (MQ=255) tctGTTGGGGATACCGCGACAACCCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAg                                                                                                                           >  2:876331/1‑140 (MQ=255)  ctGTTGGGGATACTGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGc                                                                                                                          >  1:706083/1‑140 (MQ=255)  ctGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACAATTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGc                                                                                                                          >  2:358921/1‑140 (MQ=255)  ctGTTGGAGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGc                                                                                                                          >  2:603895/1‑140 (MQ=255)    gTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGccc                                                                                                                        >  2:716572/1‑140 (MQ=255)      tGGGGATACCGCGACAACGCGGGTAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAg                                                                                                                      >  2:577486/1‑140 (MQ=255)        gggATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTa                                                                                                                    >  2:249278/1‑140 (MQ=255)        gggATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTa                                                                                                                    <  2:803784/140‑1 (MQ=255)         ggATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAg                                                                                                                   >  2:28207/1‑140 (MQ=255)          gATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGc                                                                                                                  <  1:716572/140‑1 (MQ=255)               cgcgACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAg                                                                                                                      >  1:265614/1‑131 (MQ=255)               cgcgACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAg                                                                                                                      <  2:265614/131‑1 (MQ=255)               cgcgACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACAt                                                                                                             >  1:521940/1‑140 (MQ=255)               cgcgACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACAt                                                                                                             >  1:937621/1‑140 (MQ=255)               cgcgACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACAt                                                                                                             >  2:539849/1‑140 (MQ=255)                  gACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATaaa                                                                                                          >  1:332799/1‑140 (MQ=255)                   aCAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAAc                                                                                                         >  2:200784/1‑140 (MQ=255)                      aCGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGt                                                                                                      <  2:356506/140‑1 (MQ=255)                       cgcgGGCAAATGTACCACATTTGTCAATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTg                                                                                                     >  2:532520/1‑140 (MQ=255)                         cgGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTgcg                                                                                                   <  2:736133/140‑1 (MQ=255)                           ggCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGcc                                                                                                 <  2:153142/140‑1 (MQ=255)                           ggCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGcc                                                                                                 <  1:603895/140‑1 (MQ=255)                             cAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCaa                                                                                               <  2:55691/140‑1 (MQ=255)                             cAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCaa                                                                                               <  1:200784/140‑1 (MQ=255)                             cAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCaa                                                                                               <  1:90070/140‑1 (MQ=255)                                 tGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACAt                                                                                           <  2:937621/140‑1 (MQ=255)                                         aTTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTa                                                                                   <  2:97735/140‑1 (MQ=255)                                          tttGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTAc                                                                                  >  2:322424/1‑140 (MQ=255)                                          tttGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTAc                                                                                  <  1:539849/140‑1 (MQ=255)                                           ttGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACt                                                                                 >  2:618770/1‑140 (MQ=255)                                              tCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTc                                                                              >  2:97262/1‑140 (MQ=255)                                                 aTTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCAcc                                                                           <  1:532520/140‑1 (MQ=255)                                                 aTTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCAcc                                                                           <  1:49476/140‑1 (MQ=255)                                                     ttACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAggg                                                                       >  1:822259/1‑140 (MQ=255)                                                      tACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGt                                                                      <  2:376055/140‑1 (MQ=255)                                                           ataCCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaaaa                                                                 <  1:618770/140‑1 (MQ=255)                                                           ataCCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaaaa                                                                 <  1:1194/140‑1 (MQ=255)                                                              cccAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACaa                                                              <  1:110871/140‑1 (MQ=255)                                                               ccAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGt                                                                      >  1:887028/1‑131 (MQ=255)                                                               ccAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaaaa                                                                 <  1:398617/136‑1 (MQ=255)                                                               ccAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaaaa                                                                 >  2:398617/1‑136 (MQ=255)                                                               ccAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaa                                                                   <  2:382475/134‑1 (MQ=255)                                                               ccAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaa                                                                   >  1:382475/1‑134 (MQ=255)                                                               ccAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAAt                                                             >  2:779792/1‑140 (MQ=255)                                                               ccAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAACTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGt                                                                      <  2:887028/131‑1 (MQ=255)                                                                 aGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATAc                                                           >  2:724654/1‑140 (MQ=255)                                                                  gggCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGATGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACt                                                          >  2:784749/1‑140 (MQ=255)                                                                  gggCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACt                                                          >  2:548545/1‑140 (MQ=255)                                                                   ggCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTg                                                         >  1:654144/1‑140 (MQ=255)                                                                   ggCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTg                                                         >  2:548220/1‑140 (MQ=255)                                                                   ggCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTg                                                         >  1:433562/1‑140 (MQ=255)                                                                    gCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgt                                                        >  2:338677/1‑140 (MQ=255)                                                                       tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGt                                                                      <  1:244000/123‑1 (MQ=255)                                                                       tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGt                                                                      >  2:244000/1‑123 (MQ=255)                                                                       tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaa                                                                   <  2:120732/126‑1 (MQ=255)                                                                       tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaa                                                                   >  1:120732/1‑126 (MQ=255)                                                                       tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagc                                                     >  1:84351/1‑140 (MQ=255)                                                                       tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagc                                                     >  1:399095/1‑140 (MQ=255)                                                                       tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagc                                                     <  2:822259/140‑1 (MQ=255)                                                                         cAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagcgt                                                   >  2:992668/1‑140 (MQ=255)                                                                            aaCATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagcgtagc                                                <  1:338677/140‑1 (MQ=255)                                                                               aTAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCttt                                             <  2:607238/140‑1 (MQ=255)                                                                                 aaTCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTcc                                           <  2:399095/140‑1 (MQ=255)                                                                                  aTCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCg                                          <  1:322424/140‑1 (MQ=255)                                                                                      cATTATTAGTTACGGTTTGAATTATGAACAGAGGAGATGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCa                                      <  1:784749/140‑1 (MQ=255)                                                                                      cATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCa                                      >  1:1015435/1‑140 (MQ=255)                                                                                      cATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCa                                      <  1:548545/140‑1 (MQ=255)                                                                                      cATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCa                                      >  1:993210/1‑140 (MQ=255)                                                                                      cATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCa                                      <  1:779792/140‑1 (MQ=255)                                                                                      cATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCa                                      >  1:281698/1‑140 (MQ=255)                                                                                           ttaGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCaat                                 >  2:500619/1‑140 (MQ=255)                                                                                                 aCGGTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGc                           >  2:193161/1‑140 (MQ=255)                                                                                                   ggTTTGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAg                         >  2:889665/1‑140 (MQ=255)                                                                                                     tttGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAgcg                       <  1:577486/140‑1 (MQ=255)                                                                                                     tttGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAgcg                       >  1:695769/1‑140 (MQ=255)                                                                                                       tGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCa                     <  1:724654/140‑1 (MQ=255)                                                                                                       tGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCa                     <  1:97262/140‑1 (MQ=255)                                                                                                       tGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCa                     <  1:249278/140‑1 (MQ=255)                                                                                                       tGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCa                     <  1:122221/140‑1 (MQ=255)                                                                                                       tGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCa                     >  2:602332/1‑140 (MQ=255)                                                                                                       tGAATTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCa                     <  1:47198/140‑1 (MQ=255)                                                                                                          aTTATGAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATc                  <  1:602332/140‑1 (MQ=255)                                                                                                               gAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCccac             <  2:281698/140‑1 (MQ=255)                                                                                                               gAACAGAGGAGACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCccac             <  1:193161/140‑1 (MQ=255)                                                                                                                     aggagACGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTAc       >  2:184162/1‑140 (MQ=255)                                                                                                                       gagaCGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACcg     <  2:993210/140‑1 (MQ=255)                                                                                                                       gagaCGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACcg     <  2:1015435/140‑1 (MQ=255)                                                                                                                        agaCGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCa                     >  1:258522/1‑123 (MQ=255)                                                                                                                        agaCGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCa                     <  2:258522/123‑1 (MQ=255)                                                                                                                         gaCGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACcgcg   <  1:500619/140‑1 (MQ=255)                                                                                                                          aCGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATGCTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACCGCGa  >  2:892400/1‑140 (MQ=255)                                                                                                                          aCGGGAAAGCACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTAACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACCGCGa  >  2:533336/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                   TCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACCGCGA  >  NC_000913/2872878‑2873138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑