Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,474	A→G	G156G (GGT→GGC)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,474	0	A	G	100.0%	163.1 / NA	46	G156G (GGT→GGC)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/27);  total (19/27)

CTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCA  >  NC_000913/2907341‑2907607                                                                                                                                      |                                                                                                                                      cTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtg                                                                                                                                 <  1:906142/140‑1 (MQ=255)  tttCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtga                                                                                                                                <  1:1006156/140‑1 (MQ=255)  tttCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtga                                                                                                                                >  2:242109/1‑140 (MQ=255)    tCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtgatg                                                                                                                              >  2:912196/1‑140 (MQ=255)    tCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtgatg                                                                                                                              >  2:572561/1‑140 (MQ=255)    tCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtgatg                                                                                                                              >  1:546699/1‑140 (MQ=255)          tCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGTGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTtc                                                                                                                        <  2:480467/140‑1 (MQ=255)           cAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATACCAACGTTGTTGTCAGCGTGCTAACCGCCGTTGATGATGTtca                                                                                                                       <  2:280378/140‑1 (MQ=255)               aCTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTcatcat                                                                                                                   <  1:34850/140‑1 (MQ=255)                    tGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGaa                                                                                                              <  1:19827/140‑1 (MQ=255)                    tGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGaa                                                                                                              <  1:637464/140‑1 (MQ=255)                      ccAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAAcc                                                                                                            >  2:618820/1‑140 (MQ=255)                          gTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCa                                                                                                        <  2:463059/140‑1 (MQ=255)                             aTGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATag                                                                                                     <  1:912196/140‑1 (MQ=255)                                     cTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTg                                                                                             >  1:123569/1‑140 (MQ=255)                                       tCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGcc                                                                                           <  1:571693/140‑1 (MQ=255)                                       tCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGcc                                                                                           <  1:174009/140‑1 (MQ=255)                                              ccATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTa                                                                                    <  1:552007/140‑1 (MQ=255)                                              ccATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTa                                                                                    <  2:940493/140‑1 (MQ=255)                                               cATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAc                                                                                   >  1:330761/1‑140 (MQ=255)                                                   cGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgtt                                                                               <  2:645340/140‑1 (MQ=255)                                                          cttcttTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagttca                                                                        >  2:680509/1‑140 (MQ=255)                                                             cttTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCg                                                                     <  1:572561/140‑1 (MQ=255)                                                             cttTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCg                                                                     <  1:185785/140‑1 (MQ=255)                                                              tttCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGa                                                                    <  1:618820/140‑1 (MQ=255)                                                              tttCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGa                                                                    <  1:140193/140‑1 (MQ=255)                                                                tCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGAtg                                                                  <  2:514462/140‑1 (MQ=255)                                                                      ttttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCg                                                            >  2:464493/1‑140 (MQ=255)                                                                        ttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa                                                          >  2:972940/1‑140 (MQ=255)                                                                        ttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa                                                          >  1:548909/1‑140 (MQ=255)                                                                        ttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa                                                          >  2:14786/1‑140 (MQ=255)                                                                                aaCCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCa                                                  <  1:972940/140‑1 (MQ=255)                                                                                aaCCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCa                                                  <  1:680509/140‑1 (MQ=255)                                                                                           tCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATa                                                <  1:202736/131‑1 (MQ=255)                                                                                           tCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATa                                                >  2:202736/1‑131 (MQ=255)                                                                                           tCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTagca                                       <  2:546699/140‑1 (MQ=255)                                                                                               gAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTg                                   >  2:558281/1‑140 (MQ=255)                                                                                                  ttCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTgcag                                <  1:14786/140‑1 (MQ=255)                                                                                                          tatCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTg                        >  1:271725/1‑140 (MQ=255)                                                                                                             cAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCg                     >  1:23119/1‑140 (MQ=255)                                                                                                             cAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCg                     >  2:173552/1‑140 (MQ=255)                                                                                                               aCGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGtt                   <  2:23119/140‑1 (MQ=255)                                                                                                                       gtCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCagag           >  2:651159/1‑140 (MQ=255)                                                                                                                         cAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGAt         <  2:605215/140‑1 (MQ=255)                                                                                                                             gTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAg     <  1:242109/140‑1 (MQ=255)                                                                                                                                cTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCa  >  1:236767/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      CTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCA  >  NC_000913/2907341‑2907607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑