Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 518,551 A→G V200A (GTC→GCC)  ybbO ← NADP(+)‑dependent aldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913518,5510AG100.0% 140.8 / NA 41V200A (GTC→GCC) ybbONADP(+)‑dependent aldehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/22);  total (19/22)

GTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGAC  >  NC_000913/518416‑518686
                                                                                                                                       |                                                                                                                                       
gTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGtt                                                                                                                                     <  1:558126/140‑1 (MQ=255)
 tCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTg                                                                                                                                    >  1:85814/1‑140 (MQ=255)
         tAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCACCGTAAAGCGGGCGGCGATGCAGGGATTTTCGGCTGTTTTATCTCTTTGCGTTTGGTTGGCGTTGTCGGTGaa                                                                                                                            <  2:51572/140‑1 (MQ=255)
         tAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGaa                                                                                                                            <  1:286581/140‑1 (MQ=255)
         tAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGaa                                                                                                                            <  1:812904/140‑1 (MQ=255)
         tAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGaa                                                                                                                            <  1:943581/140‑1 (MQ=255)
          aGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAg                                                                                                                           <  2:430797/140‑1 (MQ=255)
              cATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAg                                                                                                                       >  1:63449/1‑140 (MQ=255)
               aTCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGt                                                                                                                      <  2:711264/140‑1 (MQ=255)
               aTCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGt                                                                                                                      <  2:85814/140‑1 (MQ=255)
                          ctcGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGAc                                                                                                           <  2:63449/140‑1 (MQ=255)
                                     aaGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGAc                                                                                                           >  1:273869/1‑129 (MQ=255)
                                     aaGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGAc                                                                                                           <  2:273869/129‑1 (MQ=255)
                                        cATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAgg                                                                                             <  2:561378/140‑1 (MQ=255)
                                          tGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGa                                                                                                            <  1:276602/123‑1 (MQ=255)
                                          tGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGa                                                                                                            >  2:276602/1‑123 (MQ=255)
                                           ggCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTg                                                                                          >  2:978645/1‑140 (MQ=255)
                                                     gTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTc                                                                                >  1:824615/1‑140 (MQ=255)
                                                      tcaaccaccGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTcc                                                                               >  2:730779/4‑140 (MQ=255)
                                                      tCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCt                                                                                           <  1:295603/128‑1 (MQ=255)
                                                      tCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCt                                                                                           >  2:295603/1‑128 (MQ=255)
                                                           caccGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCtgt                                                                          >  1:1004423/1‑140 (MQ=255)
                                                              cGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGgc                                                                       >  2:598050/1‑140 (MQ=255)
                                                                 ttCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCa                                                                    <  1:167252/140‑1 (MQ=255)
                                                                      gTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTcc                                                               <  2:567838/140‑1 (MQ=255)
                                                                        cccAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCAt                                                             >  2:284610/1‑140 (MQ=255)
                                                                           aaCGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATgcg                                                          <  2:1004423/140‑1 (MQ=255)
                                                                             cGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCa                                                        <  2:824615/140‑1 (MQ=255)
                                                                              gTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAg                                                       <  1:284610/140‑1 (MQ=255)
                                                                                 aaGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGc                                                    >  2:827584/1‑140 (MQ=255)
                                                                                        cggcgATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTcc                                                               >  2:263659/1‑122 (MQ=255)
                                                                                        cggcgATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTcc                                                               <  1:263659/122‑1 (MQ=255)
                                                                                               gCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCt                                      <  2:334093/140‑1 (MQ=255)
                                                                                                       tttCGACTGGTTTATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCa                              <  2:760841/140‑1 (MQ=255)
                                                                                                                   tATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAg                                  >  2:413758/1‑124 (MQ=255)
                                                                                                                   tATCACTTTGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAg                                  <  1:413758/124‑1 (MQ=255)
                                                                                                                         tttGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTaa            <  2:387447/140‑1 (MQ=255)
                                                                                                                          ttGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAg           >  2:610061/1‑140 (MQ=255)
                                                                                                                          ttGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAg           <  1:827584/140‑1 (MQ=255)
                                                                                                                           tGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAgc          >  1:363155/1‑140 (MQ=255)
                                                                                                                           tGCGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAgc          >  1:854028/1‑140 (MQ=255)
                                                                                                                             cGTCTGGTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAgcgc        >  2:776315/1‑140 (MQ=255)
                                                                                                                                   gTTGGCGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGAc  >  1:452693/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGAC  >  NC_000913/518416‑518686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑