| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,972,315 | C→T | T240M (ACG→ATG) | tas → | putative NADP(H)‑dependent aldo‑keto reductase Tas |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,972,315 | 0 | C | T | 95.9% | 165.1 / ‑0.4 | 49 | coding (719/1041 nt) | tas | putative NADP(H)‑dependent aldo‑keto reductase Tas |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (21/26); minor base . (1/1); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
CACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCAT > NC_000913/2972182‑2972448 | cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGAc < 2:957777/140‑1 (MQ=255)cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGAc < 1:444466/140‑1 (MQ=255) aTCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCggg > 1:960660/1‑140 (MQ=255) tCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGa < 2:960660/140‑1 (MQ=255) tCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGa < 2:556989/140‑1 (MQ=255) tATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCa < 1:389792/140‑1 (MQ=255) tCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGTAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCA‑GCTGACCGGGAAATATCTCAATGGTg > 2:424007/1‑140 (MQ=255) tCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGt > 2:144429/1‑140 (MQ=255) aCCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGc > 2:520769/1‑140 (MQ=255) ccTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTgg < 1:38488/140‑1 (MQ=255) ccTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTgg < 1:964422/140‑1 (MQ=255) cTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgc < 2:935666/140‑1 (MQ=255) cTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgc < 2:510539/140‑1 (MQ=255) cTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgc < 1:520769/140‑1 (MQ=255) ttACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcg > 2:650685/1‑140 (MQ=255) tACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc < 2:477188/140‑1 (MQ=255) tACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc < 1:144429/140‑1 (MQ=255) tACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc < 1:721203/140‑1 (MQ=255) aCAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGTAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCA‑GCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCAc < 1:424007/140‑1 (MQ=255) aCAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCa > 2:358529/1‑140 (MQ=255) tGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAAt > 1:77958/1‑140 (MQ=255) tGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAAt < 1:275885/140‑1 (MQ=255) gTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATa < 1:915231/140‑1 (MQ=255) gTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATa > 2:915231/1‑140 (MQ=255) ttAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATAc > 1:207172/1‑140 (MQ=255) ttAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATAc < 1:358529/140‑1 (MQ=255) cGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc < 1:380200/125‑1 (MQ=255) cGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGgcgc > 2:380200/1‑125 (MQ=255) cGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATAc > 2:980605/1‑134 (MQ=255) cGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATAc < 1:980605/134‑1 (MQ=255) aGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCa < 1:650685/140‑1 (MQ=255) tAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCAcc < 2:207172/140‑1 (MQ=255) ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCAcc > 1:888155/1‑138 (MQ=255) ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCAcc < 2:888155/138‑1 (MQ=255) ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCg > 2:72774/1‑140 (MQ=255) ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCg > 2:736047/1‑140 (MQ=255) ggTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCg > 1:637332/1‑140 (MQ=255) gTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGc > 2:229239/1‑140 (MQ=255) gTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGt > 2:270123/1‑140 (MQ=255) tCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTc > 2:14414/1‑140 (MQ=255) tCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTc > 1:867915/1‑140 (MQ=255) ctgctgGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCgcggc < 1:14414/140‑1 (MQ=255) gctgGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGt > 2:827347/1‑140 (MQ=255) gctgGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGt < 2:867915/140‑1 (MQ=255) gctgGCCTATTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGt > 2:501847/1‑140 (MQ=255) aTTCGTGCCTGGGTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGat < 2:77958/140‑1 (MQ=255) ggTTTCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACg > 2:558440/1‑140 (MQ=255) tttCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTc < 2:123103/140‑1 (MQ=255) tCGGCATGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCAt < 1:501847/140‑1 (MQ=255) | CACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCAT > NC_000913/2972182‑2972448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |