Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,027,944	T→C	V275A (GTG→GCG)	ghxQ →	guanine/hypoxanthine transporter GhxQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,027,944	0	T	C	100.0%	218.0 / NA	62	V275A (GTG→GCG)	ghxQ	guanine/hypoxanthine transporter GhxQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/29);  total (33/29)

AAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATATTCTCCGGCC  >  NC_000913/3027811‑3028069                                                                                                                                      |                                                                                                                              aaaTACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGa                                                                                                                         >  1:834095/1‑140 (MQ=255)      ccACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTa                                                                                                                    <  2:118848/140‑1 (MQ=255)              tggtggCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGAc                                                                                                                        <  2:734026/128‑1 (MQ=255)              tggtggCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGAc                                                                                                                        >  1:734026/1‑128 (MQ=255)                gtggCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCAt                                                                                                          >  1:249980/1‑140 (MQ=255)                gtggCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCAt                                                                                                          >  1:825611/1‑140 (MQ=255)                      aTGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGc                                                                                                    >  2:498612/1‑140 (MQ=255)                           aaGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCg                                                                                               <  1:465273/140‑1 (MQ=255)                                  aCTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCa                                                                                        >  2:622219/1‑140 (MQ=255)                                  aCTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCa                                                                                        >  2:493021/1‑140 (MQ=255)                                       cGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGa                                                                                   >  2:527076/1‑140 (MQ=255)                                         aaGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAAt                                                                                 >  2:737260/1‑140 (MQ=255)                                             tGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATttgt                                                                             >  2:846443/1‑140 (MQ=255)                                             tGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATttgt                                                                             <  1:622219/140‑1 (MQ=255)                                             tGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATttgt                                                                             <  1:73859/140‑1 (MQ=255)                                                tAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTgg                                                                          >  2:1019880/1‑140 (MQ=255)                                                 aaGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGa                                                                         <  2:443335/140‑1 (MQ=255)                                                         gATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACa                                                                 <  2:453028/140‑1 (MQ=255)                                                            tttCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAAcc                                                              >  1:434801/1‑140 (MQ=255)                                                              tCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAg                                                            <  2:825611/140‑1 (MQ=255)                                                               cAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGa                                                           >  2:639904/1‑140 (MQ=255)                                                                  ccTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGatca                                                        >  2:791941/1‑140 (MQ=255)                                                                      gATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATaaa                                                                     <  2:246742/123‑1 (MQ=255)                                                                      gATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATaaa                                                                     >  1:246742/1‑123 (MQ=255)                                                                         attatGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAAcgg                                                 <  1:527076/140‑1 (MQ=255)                                                                              gggTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCa                                            <  2:562708/140‑1 (MQ=255)                                                                              gggTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGATGGATAAAGACAACCAGATCATCAACGGCGGCa                                            <  1:493021/140‑1 (MQ=255)                                                                                gTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCaaa                                          <  1:846443/140‑1 (MQ=255)                                                                                  gaaCTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGc                                        >  1:416849/3‑140 (MQ=255)                                                                                  gCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCa                                            >  1:124601/1‑136 (MQ=255)                                                                                  gCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCa                                            <  2:124601/136‑1 (MQ=255)                                                                                  gCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGc                                        >  2:638225/1‑140 (MQ=255)                                                                                  gCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGc                                        >  1:15661/1‑140 (MQ=255)                                                                                   cACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGcc                                       >  1:984792/1‑140 (MQ=255)                                                                                   cACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGcc                                       >  2:683396/1‑140 (MQ=255)                                                                                   cACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGcc                                       >  1:61890/1‑140 (MQ=255)                                                                                   cACTCCAGCCAACTGNACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGcc                                       >  1:583791/1‑140 (MQ=255)                                                                                    aCTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGatca                                                        <  1:515141/122‑1 (MQ=255)                                                                                    aCTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGatca                                                        >  2:515141/1‑122 (MQ=255)                                                                                    aCTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCa                                            >  1:457110/1‑134 (MQ=255)                                                                                    aCTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCa                                            <  2:457110/134‑1 (MQ=255)                                                                                    aCTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGccc                                      <  2:834095/140‑1 (MQ=255)                                                                                    aCTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGccc                                      <  2:15661/140‑1 (MQ=255)                                                                                    aCTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGccc                                      >  2:709037/1‑140 (MQ=255)                                                                                    aCTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGccc                                      <  1:954526/140‑1 (MQ=255)                                                                                           ccAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAg                               <  2:61890/140‑1 (MQ=255)                                                                                             aaCTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTg                             <  2:434801/140‑1 (MQ=255)                                                                                              aCTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGa                            <  1:683396/140‑1 (MQ=255)                                                                                              aCTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGa                            <  1:791941/140‑1 (MQ=255)                                                                                               cTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGAc                           <  2:984792/140‑1 (MQ=255)                                                                                               cTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGAc                           <  2:583791/140‑1 (MQ=255)                                                                                                tGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCATAGCCCTGACCAGTGACt                          >  1:776519/1‑140 (MQ=255)                                                                                                tGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACt                          >  2:14572/1‑140 (MQ=255)                                                                                                  tACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCa                        >  2:336185/1‑140 (MQ=255)                                                                                                      tCCGAGTGTACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTaa                    <  2:434190/140‑1 (MQ=255)                                                                                                            tgtACTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCaa              >  1:654322/1‑140 (MQ=255)                                                                                                                cTGGCATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATAtt          <  2:654322/140‑1 (MQ=255)                                                                                                                    cATTGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATATTCTcc      >  1:379943/1‑140 (MQ=255)                                                                                                                       tGGTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATATTCTCCGGc   <  1:336185/140‑1 (MQ=255)                                                                                                                        ggTGATGACCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATATTCTCCGGcc  >  2:214003/1‑140 (MQ=255)                                                                                                                             tgaCCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCATGACCAGTGACTCAGTAAGTTCAat             >  1:945003/1‑124 (MQ=255)                                                                                                                             tgaCCGCAGCGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCATGACCAGTGACTCAGTAAGTTCAat             <  2:945003/124‑1 (MQ=255)                                                                                                                                      |                                                                                                                              AAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGACGCTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATATTCTCCGGCC  >  NC_000913/3027811‑3028069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑