Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,191,392	T→C	V254A (GTC→GCC)	yqiI →	putative fimbrial protein YqiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,191,392	0	T	C	100.0%	212.6 / NA	60	V254A (GTC→GCC)	yqiI	putative fimbrial protein YqiI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/37);  total (23/37)

ATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGTCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATCTGGATATAGGTAATGGTCTGCTATTGAA  >  NC_000913/3191271‑3191512                                                                                                                          |                                                                                                                         aTTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCggg                                                                                                        >  1:349876/1‑140 (MQ=255)  ttCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGt                                                                                                       >  1:995681/1‑140 (MQ=255)  ttCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGt                                                                                                       <  1:789563/140‑1 (MQ=255)  ttCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGt                                                                                                       <  2:1001868/140‑1 (MQ=255)  ttCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGt                                                                                                       >  1:713075/1‑140 (MQ=255)  ttCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGt                                                                                                       <  2:713075/140‑1 (MQ=255)  ttCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGt                                                                                                       <  2:127446/140‑1 (MQ=255)  ttCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGt                                                                                                       <  2:463541/140‑1 (MQ=255)  ttCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGt                                                                                                       <  2:349876/140‑1 (MQ=255)    cATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGc                                                                                                     <  2:441557/140‑1 (MQ=255)     aTGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCg                                                                                                    >  1:116690/1‑140 (MQ=255)     aTGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCg                                                                                                    >  2:599735/1‑140 (MQ=255)       gTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGGCCAAAGATCCGGGTGCGGc                                                                                                  <  2:719002/140‑1 (MQ=255)       gTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGc                                                                                                  <  1:161256/140‑1 (MQ=255)       gTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGc                                                                                                  <  1:697819/140‑1 (MQ=255)       gTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGc                                                                                                  <  2:859396/140‑1 (MQ=255)         ccTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCt                                                                                                <  1:62674/140‑1 (MQ=255)         ccTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCt                                                                                                <  1:966646/140‑1 (MQ=255)          cTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGc                                                                                                  <  1:558780/137‑1 (MQ=255)          cTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGc                                                                                                  >  2:558780/1‑137 (MQ=255)          cTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGc                                                                                                  <  2:704744/137‑1 (MQ=255)          cTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGc                                                                                                  >  1:704744/1‑137 (MQ=255)          cTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTg                                                                                               >  2:814427/1‑140 (MQ=255)          cTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTg                                                                                               >  2:967792/1‑140 (MQ=255)           ttGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTATCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGt                                                                                              <  2:437435/140‑1 (MQ=255)            tGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTa                                                                                             <  2:995681/140‑1 (MQ=255)                            gTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAatat                                                                             >  1:177585/1‑140 (MQ=255)                             ttGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCACGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATAtt                                                                            >  2:622423/1‑140 (MQ=255)                                aTCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAg                                                                                        >  1:48545/1‑125 (MQ=255)                                aTCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAg                                                                                        <  2:48545/125‑1 (MQ=255)                                 tCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAg                                                                        >  1:425491/1‑140 (MQ=255)                                   tGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAggg                                                                      <  1:632160/140‑1 (MQ=255)                                   tGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAggg                                                                      <  1:339774/140‑1 (MQ=255)                                       aCCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGtt                                                                  >  2:348047/1‑140 (MQ=255)                                          aGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGTGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGttttt                                                               >  1:842863/1‑140 (MQ=255)                                           gCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGtttttt                                                              >  1:232262/1‑140 (MQ=255)                                                tGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTa                                                         <  1:814427/140‑1 (MQ=255)                                                 ggCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTAc                                                        >  2:916320/1‑140 (MQ=255)                                                 ggCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTAc                                                        >  1:375157/1‑140 (MQ=255)                                                    gTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGa                                                     <  1:939851/140‑1 (MQ=255)                                                     ttGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGat                                                    >  1:746368/1‑140 (MQ=255)                                                             tttGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCTGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAg                                            <  2:159966/140‑1 (MQ=255)                                                             tttGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAg                                            <  1:306058/140‑1 (MQ=255)                                                             tttGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAg                                            <  1:916320/140‑1 (MQ=255)                                                                 gTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGAt                                        >  2:578515/1‑140 (MQ=255)                                                                  tAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGAtt                                       >  2:207213/1‑140 (MQ=255)                                                                   aaaaTAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATta                                      <  1:419579/140‑1 (MQ=255)                                                                     aataaatTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATtata                                    <  1:53052/140‑1 (MQ=255)                                                                     aataaatTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATtata                                    <  2:10816/140‑1 (MQ=255)                                                                          atTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCAt                               <  2:375157/140‑1 (MQ=255)                                                                           ttCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATc                              <  1:578515/140‑1 (MQ=255)                                                                           ttCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATc                              <  1:395271/140‑1 (MQ=255)                                                                           ttCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATc                              <  2:746368/140‑1 (MQ=255)                                                                           ttCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATc                              <  2:984552/140‑1 (MQ=255)                                                                                gAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATCTGGat                         <  2:116690/140‑1 (MQ=255)                                                                                gAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATCTGGat                         <  1:205045/140‑1 (MQ=255)                                                                                 aaCAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATCTGGata                        >  1:995348/1‑140 (MQ=255)                                                                                             tACCAACGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATCTGGATATAGGTAATGGTc            <  1:892046/140‑1 (MQ=255)                                                                                               ccAGCGTGAGTGAGACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATCTGGATATAGGTAATGGTCTg          <  1:599735/140‑1 (MQ=255)                                                                                                       agtgagACGTTTAGCGTAGCCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATCTGGATATAGGTAATGGTCTGCTATTGaa  >  2:256693/1‑140 (MQ=255)                                                                                                                          |                                                                                                                         ATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATACCAGCGTGAGTGAGACGTTTAGCGTAGTCATGACCAAAGATCCGGGTGCGGCCTGTACTGAGCAGTTTAATATTTTAGGGAGTTTTTTCACTACGGATATTTTGAGTGATTATAGCCATCTGGATATAGGTAATGGTCTGCTATTGAA  >  NC_000913/3191271‑3191512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑