Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,237,681	C→T	A124V (GCC→GTC)	ygjR →	putative oxidoreductase YgjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,237,681	0	C	T	100.0%	112.9 / NA	33	A124V (GCC→GTC)	ygjR	putative oxidoreductase YgjR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/20);  total (13/20)

CAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTT  >  NC_000913/3237551‑3237813                                                                                                                                   |                                                                                                                                     caACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCtgcctgcc                                                                                                                             <  1:26090/140‑1 (MQ=255)              gCCATAAAATTAATGTGATTTGCGAGAACCCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAACCCGTCTGCCTGCCGAACTTTCATttg                                                                                                                <  2:150984/140‑1 (MQ=255)               ccATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATttgt                                                                                                               >  1:273128/1‑140 (MQ=255)                  tAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTgc                                                                                                            >  2:616079/1‑140 (MQ=255)                        tAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAgg                                                                                                      <  1:794980/140‑1 (MQ=255)                         aaTGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGgc                                                                                                     >  2:714524/1‑140 (MQ=255)                          aTGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGgcg                                                                                                    <  1:867190/140‑1 (MQ=255)                          aTGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGgcg                                                                                                    >  2:804407/1‑140 (MQ=255)                               aTTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGcc                                                                                               >  1:215506/1‑140 (MQ=255)                               aTTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGcc                                                                                               >  2:62508/1‑140 (MQ=255)                               aTTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGcc                                                                                               >  1:364879/1‑140 (MQ=255)                                            cGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCaaa                                                                                  <  1:419003/140‑1 (MQ=255)                                                 gCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCg                                                                             >  1:928404/1‑140 (MQ=255)                                                             gCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCttttt                                                                 <  1:714524/140‑1 (MQ=255)                                                                gAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCaa                                                              <  2:215506/140‑1 (MQ=255)                                                                    tGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTAt                                                          <  2:870763/140‑1 (MQ=255)                                                                       aTGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGTTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGc                                                       >  1:903656/1‑140 (MQ=255)                                                                           cgccATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGt                                                   >  2:611741/1‑140 (MQ=255)                                                                                   ccTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTcg                                           <  1:616079/140‑1 (MQ=255)                                                                                        gcgcGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTa                                      <  1:611741/140‑1 (MQ=255)                                                                                        gcgcGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCA‑TTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTAt                                     <  1:714865/140‑1 (MQ=255)                                                                                           cgGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCa                                   <  2:666189/140‑1 (MQ=255)                                                                                            gggAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTcg                                           <  1:628431/131‑1 (MQ=255)                                                                                            gggAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTcg                                           >  2:628431/1‑131 (MQ=255)                                                                                            gggAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCaa                                  <  2:518149/140‑1 (MQ=255)                                                                                             ggAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAAc                                 <  2:928404/140‑1 (MQ=255)                                                                                             ggAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAAc                                 <  2:624013/140‑1 (MQ=255)                                                                                                 aaTCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTa                             <  2:273128/140‑1 (MQ=255)                                                                                                   tCAGGTGGTGCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTAcc                           <  1:546787/140‑1 (MQ=255)                                                                                                        tggtgCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGAt                      <  1:62508/140‑1 (MQ=255)                                                                                                         ggtgCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTAt                                     <  2:573051/124‑1 (MQ=255)                                                                                                         ggtgCTGTTTGAGGCATTTAAAACCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTAt                                     >  1:573051/1‑124 (MQ=255)                                                                                                                            aaaaCCGTCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCtt  >  1:217954/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                     CAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTT  >  NC_000913/3237551‑3237813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑