Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,245,633	G→A	G137D (GGC→GAC)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,245,633	0	G	A	100.0%	235.2 / NA	69	G137D (GGC→GAC)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/44);  total (25/44)

CCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTG  >  NC_000913/3245498‑3245769                                                                                                                                        |                                                                                                                                         ccGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTAc                                                                                                                                      <  1:439683/140‑1 (MQ=255) ccGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTAc                                                                                                                                      <  1:423158/140‑1 (MQ=255)      cTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTAGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTaa                                                                                                                                 >  1:429642/1‑140 (MQ=255)        ggCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACg                                                                                                                               >  1:790725/1‑140 (MQ=255)          cAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTa                                                                                                                             <  1:996350/140‑1 (MQ=255)          cAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTa                                                                                                                             <  1:955072/140‑1 (MQ=255)           aGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAg                                                                                                                            <  2:512165/140‑1 (MQ=255)             gTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGt                                                                                                                          <  2:12461/140‑1 (MQ=255)              tAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGtt                                                                                                                         >  1:937319/1‑140 (MQ=255)               aGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGttc                                                                                                                        <  2:790725/140‑1 (MQ=255)               aGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGttc                                                                                                                        <  1:126933/140‑1 (MQ=255)               aGCTCGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGttc                                                                                                                        <  2:937319/140‑1 (MQ=255)                  tGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGttcttc                                                                                                                     <  1:251881/140‑1 (MQ=255)                   ggggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCg                                                                                                                    >  1:469365/1‑140 (MQ=255)                   ggggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCg                                                                                                                    >  2:386971/1‑140 (MQ=255)                     ggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGAt                                                                                                                  <  2:768905/140‑1 (MQ=255)                     ggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGAt                                                                                                                  <  1:640753/140‑1 (MQ=255)                          ccTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGGTCCCGGCGAAAAAGCGGTCCATCGCAGTAGACTACTTTAACGTAGGGTCTTCGATTGGTg                                                                                                             >  2:121919/1‑140 (MQ=255)                              gCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCgat                                                                                                         >  2:778880/1‑140 (MQ=255)                                   tGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTg                                                                                                    >  2:638448/1‑140 (MQ=255)                                   tGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTg                                                                                                    >  1:533956/1‑140 (MQ=255)                                   tGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTg                                                                                                    >  2:159672/1‑140 (MQ=255)                                         tggtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgc                                                                                              <  2:424044/140‑1 (MQ=255)                                         tggtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgc                                                                                              <  1:778880/140‑1 (MQ=255)                                           gtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgccg                                                                                            <  2:496048/140‑1 (MQ=255)                                           gtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgccg                                                                                            <  2:572847/140‑1 (MQ=255)                                            tgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgccgc                                                                                           >  2:893728/1‑140 (MQ=255)                                              cggtcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtg                                                                                         <  1:580206/140‑1 (MQ=255)                                              cggtcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtg                                                                                         <  1:419050/140‑1 (MQ=255)                                              cggtcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtg                                                                                         <  1:893728/140‑1 (MQ=255)                                               ggtcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtgg                                                                                        >  2:24510/1‑140 (MQ=255)                                                      gCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATg                                                                                 <  2:469365/140‑1 (MQ=255)                                                        cgcgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATggg                                                                               <  1:638448/140‑1 (MQ=255)                                                        cgcgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATggg                                                                               <  1:159672/140‑1 (MQ=255)                                                         gcgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGc                                                                              <  1:980171/140‑1 (MQ=255)                                                         gcgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGc                                                                              <  1:24510/140‑1 (MQ=255)                                                          cgGAAGCAGCGATGATCCCGGCGGGTCTGGAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGGGGTCCATCCCAGGAGAATACTTTTACGTAAGTTCTTCGATTTGGGGGGTGATTGCGCCGCCGCTGGTGGGATgggga                                                                             >  2:259707/1‑138 (MQ=255)                                                               gcagcGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGt                                                                        >  1:752396/1‑140 (MQ=255)                                                                     gtgatCCCGGCGGGTCTGGAAGGCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGca                                                                  <  2:893420/139‑1 (MQ=255)                                                                       gatCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGcaca                                                                >  2:500250/1‑140 (MQ=255)                                                                       gatCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGcac                                                                 >  1:120420/1‑139 (MQ=255)                                                                       gatCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGcac                                                                 <  2:120420/139‑1 (MQ=255)                                                                        atCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAg                                                               <  1:121919/140‑1 (MQ=255)                                                                              gcggGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCa                                                         <  2:623850/140‑1 (MQ=255)                                                                              gcggGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGGGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCa                                                         >  2:256354/1‑140 (MQ=255)                                                                               cggGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAg                                                        >  1:696932/1‑140 (MQ=255)                                                                                  gTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATg                                                     >  1:124247/1‑140 (MQ=255)                                                                                   tCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATgg                                                    >  1:884308/1‑140 (MQ=255)                                                                                   tCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATgg                                                    <  2:477278/140‑1 (MQ=255)                                                                                    cTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGc                                                   >  1:650220/1‑140 (MQ=255)                                                                                      gAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCAt                                                 <  1:703845/140‑1 (MQ=255)                                                                                          gCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTat                                             <  2:884308/140‑1 (MQ=255)                                                                                           ccAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTatc                                            <  2:124247/140‑1 (MQ=255)                                                                                            cAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTatca                                           >  1:145336/1‑140 (MQ=255)                                                                                               cTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCAtct                                        >  2:338458/1‑140 (MQ=255)                                                                                                  cGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATTGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCg                                     <  1:430814/140‑1 (MQ=255)                                                                                                  cGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCg                                     <  2:77996/140‑1 (MQ=255)                                                                                                    aaTGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGt                                   <  2:414662/140‑1 (MQ=255)                                                                                                        gTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt                               <  2:148659/140‑1 (MQ=255)                                                                                                        gTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt                               <  2:533956/140‑1 (MQ=255)                                                                                                        gTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt                               <  2:429642/140‑1 (MQ=255)                                                                                                        gTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt                               <  1:386971/140‑1 (MQ=255)                                                                                                         ttCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAtt                              <  2:650220/140‑1 (MQ=255)                                                                                                         ttCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAtt                              <  2:752396/140‑1 (MQ=255)                                                                                                         ttCCCGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAtt                              >  1:276769/1‑140 (MQ=255)                                                                                                            ccGGCGAAAGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAg                           <  1:500250/140‑1 (MQ=255)                                                                                                                   aaGAGCGTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATc                    <  1:203518/140‑1 (MQ=255)                                                                                                                         gTTCCATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCg              <  1:56571/140‑1 (MQ=255)                                                                                                                             cATCGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatgg          >  2:906518/1‑140 (MQ=255)                                                                                                                                cGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcat       >  1:933995/1‑140 (MQ=255)                                                                                                                                cGCAGTAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcat       <  1:543235/140‑1 (MQ=255)                                                                                                                                     tAGACTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTg  >  2:144864/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         CCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTG  >  NC_000913/3245498‑3245769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑