Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,357,504	T→C	V927A (GTC→GCC)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,357,504	0	T	C	100.0%	91.4 / NA	27	V927A (GTC→GCC)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/10);  total (17/10)

GGCGGTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCAC  >  NC_000913/3357374‑3357640                                                                                                                                   |                                                                                                                                         ggcggTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGAt                                                                                                                                 <  1:329402/140‑1 (MQ=255)  gcggTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGAtt                                                                                                                                >  2:688232/1‑140 (MQ=255)  gcggTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGAtt                                                                                                                                >  1:582619/1‑140 (MQ=255)             aaCTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCa                                                                                                                     <  1:688232/140‑1 (MQ=255)                        aGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGc                                                                                                          <  2:582619/140‑1 (MQ=255)                        aGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGc                                                                                                          <  2:31566/140‑1 (MQ=255)                                             cTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGc                                                                                     >  1:682971/1‑140 (MQ=255)                                                        acaaAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATaaa                                                                          >  2:292201/1‑140 (MQ=255)                                                            aGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCa                                                                      >  1:532938/1‑140 (MQ=255)                                                             gtgtCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCAc                                                                     >  1:676377/1‑140 (MQ=255)                                                                tCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGcc                                                                  >  2:6583/1‑140 (MQ=255)                                                                        cAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCg                                                          >  1:379037/1‑140 (MQ=255)                                                                                         gTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCg                                         >  1:516050/1‑140 (MQ=255)                                                                                                        cTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACg                          >  1:238474/1‑140 (MQ=255)                                                                                                        cTCCGGCGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACg                          >  2:616203/1‑140 (MQ=255)                                                                                                              cGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAg                               >  1:616220/1‑129 (MQ=255)                                                                                                              cGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAg                               <  2:616220/129‑1 (MQ=255)                                                                                                              cGTATCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGAtc                    >  1:803392/1‑140 (MQ=255)                                                                                                                  tCTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTccc                <  2:532938/140‑1 (MQ=255)                                                                                                                   cTGGTCAATGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTcccc               >  2:101266/1‑140 (MQ=255)                                                                                                                           tGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGAt                     <  2:236039/126‑1 (MQ=255)                                                                                                                           tGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGAt                     >  1:236039/1‑126 (MQ=255)                                                                                                                           tGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCcgccgccgc       >  2:51939/1‑140 (MQ=255)                                                                                                                           tGCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCcgccgccgc       >  1:451134/1‑140 (MQ=255)                                                                                                                            gCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGca      <  2:451134/140‑1 (MQ=255)                                                                                                                            gCCGACGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGca      <  1:51939/140‑1 (MQ=255)                                                                                                                                aCGCCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGcaccac  <  2:682971/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         GGCGGTAATTCGAACTCCGGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCAC  >  NC_000913/3357374‑3357640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑