Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,726	A→G	V134A (GTT→GCT)	aaeA ←	aromatic carboxylic acid efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,726	0	A	G	100.0%	137.2 / NA	39	V134A (GTT→GCT)	aaeA	aromatic carboxylic acid efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/24);  total (15/24)

TCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCT  >  NC_000913/3388590‑3388854                                                                                                                                         |                                                                                                                                 tCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCg                                                                                                                               <  2:166851/140‑1 (MQ=255)  ccTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGt                                                                                                                              <  2:560276/140‑1 (MQ=255)          aataaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTgtagta                                                                                                                      <  1:689288/140‑1 (MQ=255)          aataaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTgtagta                                                                                                                      <  2:45910/140‑1 (MQ=255)            taaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACg                                                                                                                    >  1:672890/1‑140 (MQ=255)             aaaCTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACgt                                                                                                                   <  2:389595/140‑1 (MQ=255)              aaCTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACgtt                                                                                                                  <  2:672890/140‑1 (MQ=255)               aCTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACgttg                                                                                                                 <  2:596627/140‑1 (MQ=255)                  cACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGttgttg                                                                                                              >  1:411683/1‑140 (MQ=255)                   aCCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTgg                                                                                                             >  1:783302/1‑140 (MQ=255)                     cGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGcc                                                                                                           <  2:924931/140‑1 (MQ=255)                                 tGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATctct                                                                                               <  2:287899/140‑1 (MQ=255)                                  gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTg                                                                                                         <  2:915784/129‑1 (MQ=255)                                  gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTg                                                                                                         >  1:915784/1‑129 (MQ=255)                                  gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCtt                                                                                              >  2:374790/1‑140 (MQ=255)                                    ggTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTcg                                                                                            >  1:833204/1‑140 (MQ=255)                                          tCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGAc                                                                                      <  1:325605/140‑1 (MQ=255)                                                 gCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCt                                                                               <  1:398544/140‑1 (MQ=255)                                                    aTCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGca                                                                            >  2:591338/1‑140 (MQ=255)                                                     tCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGcac                                                                           >  1:162476/1‑140 (MQ=255)                                                     tCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGcac                                                                           >  1:608380/1‑140 (MQ=255)                                                          tgGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCga                                                                      <  2:272187/140‑1 (MQ=255)                                                          tgGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCga                                                                      <  2:960567/140‑1 (MQ=255)                                                              gcgcgGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACg                                                                  <  2:608380/140‑1 (MQ=255)                                                                    aTCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTacg                                                            <  2:281179/140‑1 (MQ=255)                                                                           tGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCg                                                     <  2:833204/140‑1 (MQ=255)                                                                             cGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGc                                                   >  2:742541/1‑140 (MQ=255)                                                                              gTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcc                                                  >  2:590969/1‑140 (MQ=255)                                                                                             ttGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTcc                                   <  1:742541/140‑1 (MQ=255)                                                                                             ttGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTcc                                   <  1:466474/140‑1 (MQ=255)                                                                                              tGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGATGTCCGGCCTCCTGGCGTTTCTCCt                                  <  2:779757/140‑1 (MQ=255)                                                                                              tGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCt                                  <  2:498902/140‑1 (MQ=255)                                                                                                    aTCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCa                            <  2:881287/140‑1 (MQ=255)                                                                                                              gCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGata                  <  2:767755/140‑1 (MQ=255)                                                                                                              gCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGata                  >  1:865704/1‑140 (MQ=255)                                                                                                               ccTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGataa                 <  2:484368/140‑1 (MQ=255)                                                                                                                   cgcTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAg             >  2:847753/1‑140 (MQ=255)                                                                                                                     cTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCa           >  1:467800/1‑140 (MQ=255)                                                                                                                              aCTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCt  >  1:743599/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                 TCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCT  >  NC_000913/3388590‑3388854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑