Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,819,380	C→T	N169N (AAC→AAT)	yicG →	conserved inner membrane protein YicG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,819,380	0	C	T	100.0%	187.6 / NA	53	N169N (AAC→AAT)	yicG	conserved inner membrane protein YicG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/25);  total (28/25)

CACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCT  >  NC_000913/3819245‑3819513                                                                                                                                        |                                                                                                                                      caccGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCatg                                                                                                                                   <  2:834329/140‑1 (MQ=255) caccGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCatg                                                                                                                                   <  2:207354/140‑1 (MQ=255)    cGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCatgatg                                                                                                                                <  2:611453/140‑1 (MQ=255)     ggCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGAtgt                                                                                                                               >  1:483288/1‑140 (MQ=255)      gCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCatgatg                                                                                                                                <  2:290460/138‑1 (MQ=255)      gCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCatgatg                                                                                                                                >  1:290460/1‑138 (MQ=255)       cGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGAATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTgg                                                                                                                             <  1:178369/140‑1 (MQ=255)           tttGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAAt                                                                                                                         <  2:842629/140‑1 (MQ=255)           tttGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAAt                                                                                                                         <  1:875897/140‑1 (MQ=255)                cggcgTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTAtct                                                                                                                    >  2:334513/1‑140 (MQ=255)                     ttttGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCAcc                                                                                                               >  2:635476/1‑140 (MQ=255)                               tatGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGCAATTATCTCCACCCTGGTATTCg                                                                                                     >  1:83561/1‑140 (MQ=255)                                     cTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCtttt                                                                                               >  2:606438/1‑140 (MQ=255)                                        tAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTg                                                                                            >  1:215627/1‑140 (MQ=255)                                         aaaCGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGc                                                                                           >  2:582664/1‑140 (MQ=255)                                                  ccACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACt                                                                                  >  2:280060/1‑140 (MQ=255)                                                     cTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGgc                                                                               >  1:992174/1‑140 (MQ=255)                                                       ggTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGgcgc                                                                             <  2:992174/140‑1 (MQ=255)                                                           ttCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACg                                                                         >  2:656664/1‑140 (MQ=255)                                                              cAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCt                                                                      <  1:30589/140‑1 (MQ=255)                                                                  aaGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAg                                                                  <  1:871635/140‑1 (MQ=255)                                                                   agagTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGc                                                                 >  2:362025/1‑140 (MQ=255)                                                                      gTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTgg                                                              >  1:604968/1‑140 (MQ=255)                                                                        tGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGa                                                            >  2:103697/1‑140 (MQ=255)                                                                           aTGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTa                                                         >  2:586204/1‑140 (MQ=255)                                                                            tGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGCAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTAc                                                        <  2:83561/140‑1 (MQ=255)                                                                              ccGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCg                                                      <  1:586204/140‑1 (MQ=255)                                                                              ccGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCg                                                      <  2:604968/140‑1 (MQ=255)                                                                               cGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCgg                                                     <  2:215627/140‑1 (MQ=255)                                                                               cGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCgg                                                     <  1:656664/140‑1 (MQ=255)                                                                               cGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCgg                                                     >  2:188013/1‑140 (MQ=255)                                                                                ggTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGt                                                    >  2:697405/1‑140 (MQ=255)                                                                                    tctcATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTAc                                                        <  1:80795/132‑1 (MQ=255)                                                                                    tctcATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTAc                                                        >  2:80795/1‑132 (MQ=255)                                                                                    tctcATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCttt                                                >  1:301954/1‑140 (MQ=255)                                                                                    tctcATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCttt                                                >  1:900584/1‑140 (MQ=255)                                                                                         tttGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTtacta                                           <  1:188013/140‑1 (MQ=255)                                                                                              cTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGcc                                      <  1:606438/140‑1 (MQ=255)                                                                                              cTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGcc                                      <  1:103697/140‑1 (MQ=255)                                                                                               tCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCa                                     <  1:571786/140‑1 (MQ=255)                                                                                                ccgccgTTGTGTATATCTCGCTCCCACTCTATCTTTCTAAACATTTTTTGGTATTTATATCCGCCCTGCTAGTCGTCTTTTTTGCCCGATTACTGGCGCTACGCCTTAAAATGGGATTACCGGTCTTTTACTAGAGGCAc                                    >  2:660233/1‑140 (MQ=255)                                                                                                 cgccgTGCTGTACATCGCGCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCACTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACg                                   <  1:9800/140‑1 (MQ=255)                                                                                                                cgcgCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTa                         <  1:983426/135‑1 (MQ=255)                                                                                                                cgcgCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTa                         >  2:983426/1‑135 (MQ=255)                                                                                                                cgcgCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGtt                    >  2:456135/1‑140 (MQ=255)                                                                                                                cgcgCTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGtt                    <  1:697405/140‑1 (MQ=255)                                                                                                                  cgcTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGa                              <  2:396064/128‑1 (MQ=255)                                                                                                                  cgcTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGa                              >  1:396064/1‑128 (MQ=255)                                                                                                                  cgcTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCa                  >  2:405352/1‑140 (MQ=255)                                                                                                                  cgcTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCa                  >  2:360693/1‑140 (MQ=255)                                                                                                                  cgcTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCa                  >  1:997327/1‑140 (MQ=255)                                                                                                                  cgcTGCAACACTATGTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCa                  >  2:862442/1‑140 (MQ=255)                                                                                                                           acTATCTTTCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCtgtg         <  2:900584/140‑1 (MQ=255)                                                                                                                                  ttCTAATCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATctgct  >  1:97654/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTATCCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCT  >  NC_000913/3819245‑3819513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑