Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,846,404	A→G	V255A (GTG→GCG)	uhpT ←	hexose‑6‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,846,404	0	A	G	100.0%	163.2 / NA	46	V255A (GTG→GCG)	uhpT	hexose‑6‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/25);  total (21/25)

CCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAG  >  NC_000913/3846269‑3846540                                                                                                                                        |                                                                                                                                         cccTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATa                                                                                                                                      <  1:104214/140‑1 (MQ=255) cccTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATa                                                                                                                                      <  1:676459/140‑1 (MQ=255) cccTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATa                                                                                                                                      <  1:695061/140‑1 (MQ=255)  ccTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATAc                                                                                                                                     <  2:191435/140‑1 (MQ=255)    tGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTc                                                                                                                                   >  2:293647/1‑140 (MQ=255)      aaTCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTcaa                                                                                                                                 >  1:772776/1‑140 (MQ=255)             aCCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGAt                                                                                                                          <  1:398088/140‑1 (MQ=255)                    tAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCAc                                                                                                                   <  1:293647/140‑1 (MQ=255)                    tAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCAc                                                                                                                   <  1:485779/140‑1 (MQ=255)                    tAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCAc                                                                                                                   <  1:809404/140‑1 (MQ=255)                             tCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCata                                                                                                          <  2:772776/140‑1 (MQ=255)                             tCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCata                                                                                                          <  2:434842/140‑1 (MQ=255)                                     tGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGa                                                                                                  <  1:342292/140‑1 (MQ=255)                                                     ttGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGGCTCTTTGTcc                                                                                  >  1:699281/1‑140 (MQ=255)                                                      tGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCct                                                                                 <  1:974756/140‑1 (MQ=255)                                                       gACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGa                                                                                                  >  2:89461/1‑122 (MQ=255)                                                       gACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGa                                                                                                  <  1:89461/122‑1 (MQ=255)                                                          cACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCct                                                                                 <  1:675107/136‑1 (MQ=255)                                                          cACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCct                                                                                 >  2:675107/1‑136 (MQ=255)                                                              ggTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTg                                                                         >  2:843562/1‑140 (MQ=255)                                                               gTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGa                                                                        >  1:919846/1‑140 (MQ=255)                                                                 cGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAtc                                                                      >  2:838733/1‑140 (MQ=255)                                                                         aTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGcc                                                              >  2:788552/1‑140 (MQ=255)                                                                             gTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAAc                                                          <  2:407792/140‑1 (MQ=255)                                                                              tACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                         >  1:860470/1‑140 (MQ=255)                                                                              tACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                         >  2:530105/1‑140 (MQ=255)                                                                              tACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                         >  1:702302/1‑140 (MQ=255)                                                                                      agagGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCa                                                 >  1:77699/1‑140 (MQ=255)                                                                                        agGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGc                                               >  2:95472/1‑140 (MQ=255)                                                                                        agGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGc                                               >  1:227841/1‑140 (MQ=255)                                                                                             aaTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGc                                          >  2:344039/1‑140 (MQ=255)                                                                                              aTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTCTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGcc                                         <  2:702302/140‑1 (MQ=255)                                                                                                gTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGa                                       <  2:77699/140‑1 (MQ=255)                                                                                                  tGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAgg                                     >  1:874465/1‑140 (MQ=255)                                                                                                       aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGcc                                         >  2:666384/1‑131 (MQ=255)                                                                                                       aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGcc                                         <  1:666384/131‑1 (MQ=255)                                                                                                       aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATa                                <  1:95472/140‑1 (MQ=255)                                                                                                                 gcCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCggg                      >  2:657543/1‑140 (MQ=255)                                                                                                                 gcCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCggg                      <  1:788552/140‑1 (MQ=255)                                                                                                                  ccAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCgggg                     <  1:838733/140‑1 (MQ=255)                                                                                                                        cACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGGGTCGc               <  2:699281/140‑1 (MQ=255)                                                                                                                           tttGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGc            >  2:294483/1‑140 (MQ=255)                                                                                                                                  ttCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACg     >  2:671249/1‑140 (MQ=255)                                                                                                                                  ttCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACg     <  2:256762/140‑1 (MQ=255)                                                                                                                                     aGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAg  <  1:710234/140‑1 (MQ=255)                                                                                                                                     aGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAg  <  2:919846/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAG  >  NC_000913/3846269‑3846540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑