Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,941,273	C→T	A19T (GCT→ACT)	yieP ←	putative transcriptional regulator YieP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,941,273	0	C	T	100.0%	186.6 / NA	53	A19T (GCT→ACT)	yieP	putative transcriptional regulator YieP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/36);  total (17/36)

TTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCAAAAACGGCAAA  >  NC_000913/3941136‑3941404                                                                                                                                          |                                                                                                                                    ttttGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCa                                                                                                                                   <  2:208994/140‑1 (MQ=255)  tttGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCtcagtcag                                                                                                                                  <  1:386049/140‑1 (MQ=255)     gCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAAc                                                                                                                               <  2:378614/140‑1 (MQ=255)         ttAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATAc                                                                                                                           >  2:36906/1‑140 (MQ=255)          tAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACg                                                                                                                          <  1:950978/140‑1 (MQ=255)          tAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACg                                                                                                                          <  2:474939/140‑1 (MQ=255)          tAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACg                                                                                                                          <  2:870736/140‑1 (MQ=255)           aaCGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGa                                                                                                                         >  2:856356/1‑140 (MQ=255)               ttttGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAgg                                                                                                                     <  1:883105/140‑1 (MQ=255)                tttGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGt                                                                                                                    >  2:945192/1‑140 (MQ=255)                      cGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCt                                                                                                              <  2:467229/140‑1 (MQ=255)                         ttCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           <  1:36906/140‑1 (MQ=255)                         ttCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           <  2:589963/140‑1 (MQ=255)                         ttCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           <  2:920849/140‑1 (MQ=255)                           cgcgTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCa                                                                                                         <  1:559804/140‑1 (MQ=255)                             cgTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           >  1:314789/1‑136 (MQ=255)                             cgTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCtgtg                                                                                                           <  2:314789/136‑1 (MQ=255)                                  cGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACt                                                                                                  >  2:524971/1‑140 (MQ=255)                                   gCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACtg                                                                                                 >  2:85837/1‑140 (MQ=255)                                       tACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACtgttgt                                                                                             >  2:552066/1‑140 (MQ=255)                                       tACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACtgttgt                                                                                             <  1:107455/140‑1 (MQ=255)                                        aCGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTtgtg                                                                                            <  1:221291/140‑1 (MQ=255)                                            ctcactcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCt                                                                                        >  2:840893/1‑140 (MQ=255)                                             tcactcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCtt                                                                                       <  2:456179/140‑1 (MQ=255)                                              cacgcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTGTTAAGATCCAATGCGCCCGCTTCTCAGTCAGATCATACGCAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTa                                                                                      <  2:575144/140‑1 (MQ=255)                                                 tcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTtgtg                                                                                            >  2:878854/1‑131 (MQ=255)                                                 tcCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTtgtg                                                                                            <  1:878854/131‑1 (MQ=255)                                                  ccAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAgg                                                                                  <  1:809757/140‑1 (MQ=255)                                                     aaTTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAt                                                                               <  1:300336/140‑1 (MQ=255)                                                      aTTGCTCGCCCAGCTCAATCTCACTGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAtt                                                                              >  2:58229/1‑140 (MQ=255)                                                                        tctcACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttct                                                            <  2:41619/140‑1 (MQ=255)                                                                             ccGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTa                                                       >  2:529592/1‑140 (MQ=255)                                                                              cGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAt                                                      <  2:679667/140‑1 (MQ=255)                                                                               gggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAtt                                                     <  1:58229/140‑1 (MQ=255)                                                                               gggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAtt                                                     <  1:552066/140‑1 (MQ=255)                                                                               gggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAtt                                                     <  1:85837/140‑1 (MQ=255)                                                                                ggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATtc                                                    <  1:524971/140‑1 (MQ=255)                                                                                ggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATtc                                                    <  1:840893/140‑1 (MQ=255)                                                                                ggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATtc                                                    <  1:529592/140‑1 (MQ=255)                                                                                     aaTGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTcctcct                                               >  1:262824/1‑140 (MQ=255)                                                                                      aTGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCtt                                              <  2:857535/140‑1 (MQ=255)                                                                                          tGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTa                                          <  1:343182/140‑1 (MQ=255)                                                                                          tGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTa                                          <  1:956616/140‑1 (MQ=255)                                                                                          tGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTa                                          <  2:463389/140‑1 (MQ=255)                                                                                                    atatTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAgg                                <  1:586018/140‑1 (MQ=255)                                                                                                    atatTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAgg                                <  1:945192/140‑1 (MQ=255)                                                                                                       ttCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAg                             <  2:262824/140‑1 (MQ=255)                                                                                                          aCCTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAgtgt                          >  1:964651/1‑140 (MQ=255)                                                                                                           ccTTTTAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGtgtg                         >  1:593761/1‑140 (MQ=255)                                                                                                               ttAAGATCCGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTa                     <  1:299772/140‑1 (MQ=255)                                                                                                                      ccGTTGCGCCAGCTTCTCTGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTCTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGc              >  2:380642/1‑140 (MQ=255)                                                                                                                      ccGTTGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGc              >  2:856909/1‑140 (MQ=255)                                                                                                                         ttGCGCCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCaaa           >  2:659067/1‑140 (MQ=255)                                                                                                                             gcCAGCTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCAAAAACg       <  2:964651/140‑1 (MQ=255)                                                                                                                                  cTTCTCAGTCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCAAAAACGGCaaa  <  2:593761/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    TTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGGAAGTGTGATTACGGTTGCAAAAACGGCAAA  >  NC_000913/3941136‑3941404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑