Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	625,143	T→C	S87P (TCG→CCG)	entC →	isochorismate synthase EntC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	625,143	0	T	C	100.0%	140.1 / NA	40	S87P (TCG→CCG)	entC	isochorismate synthase EntC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

CCGGCTGTGAACGGGGATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGTCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGCCAGGCAATTCCGGAGCAAACCACGTTTGAACA  >  NC_000913/625008‑625276                                                                                                                                        |                                                                                                                                      ccGGCTGTGAACGGGGATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCt                                                                                                                                   <  1:964179/140‑1 (MQ=255)  cGGCTGTGAACGGGGATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTg                                                                                                                                  >  2:79860/1‑140 (MQ=255)    gCTGTGAACGGGGATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGta                                                                                                                                <  2:350273/140‑1 (MQ=255)      tgtgAACGGGGATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCATGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGtata                                                                                                                              >  2:59573/1‑140 (MQ=255)              gggATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGa                                                                                                                       <  1:143911/139‑1 (MQ=255)              gggATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGa                                                                                                                       >  2:143911/1‑139 (MQ=255)               ggATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAAt                                                                                                                     >  1:986907/1‑140 (MQ=255)                    cGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCATGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTgg                                                                                                                <  1:59573/140‑1 (MQ=255)                     gCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGc                                                                                                               <  2:811139/140‑1 (MQ=255)                     gCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGc                                                                                                               <  2:277578/140‑1 (MQ=255)                     gCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGc                                                                                                               >  1:811139/1‑140 (MQ=255)                         gACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTc                                                                                                           <  2:986907/140‑1 (MQ=255)                            aGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGtt                                                                                                        <  1:60720/140‑1 (MQ=255)                              tCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGc                                                                                                               <  1:166381/131‑1 (MQ=255)                              tCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGc                                                                                                               >  2:166381/1‑131 (MQ=255)                                   tCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGt                                                                                                 <  1:238232/140‑1 (MQ=255)                                     cAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCa                                                                                               >  1:548295/1‑140 (MQ=255)                                                          tttGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACg                                                                          <  1:1010495/140‑1 (MQ=255)                                                          tttGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACg                                                                          <  2:858318/140‑1 (MQ=255)                                                          tttGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACg                                                                          <  2:287427/140‑1 (MQ=255)                                                                gATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGttt                                                                    <  1:135010/140‑1 (MQ=255)                                                                gATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGttt                                                                    <  1:79860/140‑1 (MQ=255)                                                                    ccAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCAcc                                                                >  1:366530/1‑140 (MQ=255)                                                                    ccAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCAcc                                                                >  1:374794/1‑140 (MQ=255)                                                                             aGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAg                                                       <  1:539080/140‑1 (MQ=255)                                                                                    cAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCAc                                                                 >  1:584932/1‑123 (MQ=255)                                                                                    cAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCAc                                                                 <  2:584932/123‑1 (MQ=255)                                                                                    cAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGa                                                >  1:339964/1‑140 (MQ=255)                                                                                       aaaTCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAAtg                                             <  2:708181/140‑1 (MQ=255)                                                                                         aTCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAACCAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCt                                                  >  2:174111/1‑133 (MQ=255)                                                                                         aTCCGGTGATGGTCGGGGCGATTCCCGTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCt                                                  <  1:174111/133‑1 (MQ=255)                                                                                           ccGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATgtggt                                         >  1:663499/1‑140 (MQ=255)                                                                                            cGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATgtggtg                                        <  2:339964/140‑1 (MQ=255)                                                                                               tGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGaa                                     >  2:410732/1‑140 (MQ=255)                                                                                                      cGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGCCAgg                              <  1:410732/140‑1 (MQ=255)                                                                                                             aTTCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGCCAGGCAATTcc                       >  1:947245/1‑140 (MQ=255)                                                                                                              ttCCCTTCGATCCACGTCAGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGCCAGGCAATTCCg                      >  2:844719/1‑140 (MQ=255)                                                                                                                                aGCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGCCAGGCAATTCCGGAGCAAACCACGTTTGaa    >  2:976812/1‑140 (MQ=255)                                                                                                                                 gCCTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGCCAGGCAATTCCGGAGCAAACCACGTTTGAAc   >  2:982590/1‑140 (MQ=255)                                                                                                                                  ccTTCGCCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGCCAGGCAATTCCGGAGCAAACCACGTTTGAACa  >  2:602221/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CCGGCTGTGAACGGGGATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTGTTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTCGATCCACGTCAGCCTTCGTCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAGGAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGCCAGGCAATTCCGGAGCAAACCACGTTTGAACA  >  NC_000913/625008‑625276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑