Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,183,301	A→G	Q686R (CAA→CGA)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,183,301	0	A	G	100.0%	293.2 / NA	82	Q686R (CAA→CGA)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/43);  total (39/43)

CCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCC  >  NC_000913/4183169‑4183434                                                                                                                                     |                                                                                                                                      ccAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAg                                                                                                                                <  1:871733/140‑1 (MQ=255)   aGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGc                                                                                                                              <  2:432050/140‑1 (MQ=255)     cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCg                                                                                                                            >  1:928489/1‑140 (MQ=255)         ttCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTcc                                                                                                                        >  1:562588/1‑140 (MQ=255)          tCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCg                                                                                                                       <  2:894301/140‑1 (MQ=255)              ccGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGActc                                                                                                                   >  2:327711/1‑140 (MQ=255)               cgcgACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGActct                                                                                                                  >  2:884583/1‑140 (MQ=255)                   aCCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGc                                                                                                                              >  2:72567/1‑124 (MQ=255)                   aCCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGc                                                                                                                              <  1:72567/124‑1 (MQ=255)                   aCCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTgcgc                                                                                                              >  1:161645/1‑140 (MQ=255)                   aCCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTgcgc                                                                                                              >  2:582784/1‑140 (MQ=255)                    ccAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTgcgcg                                                                                                             <  1:23004/140‑1 (MQ=255)                    ccAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTgcgcg                                                                                                             <  2:161645/140‑1 (MQ=255)                         ttGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGAt                                                                                                        <  2:266530/140‑1 (MQ=255)                           gACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATaa                                                                                                      <  2:562588/140‑1 (MQ=255)                            aCTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAg                                                                                                     >  1:732133/1‑140 (MQ=255)                               aCATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                                                                                                  <  2:928489/140‑1 (MQ=255)                                  tGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtg                                                                                               >  2:329065/1‑140 (MQ=255)                                  tGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtg                                                                                               >  2:163083/1‑140 (MQ=255)                                  tGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtg                                                                                               >  1:454051/1‑140 (MQ=255)                                  tGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtg                                                                                               >  2:597104/1‑140 (MQ=255)                                  tGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtg                                                                                               <  1:623516/140‑1 (MQ=255)                                      cGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttg                                                                                           >  1:154361/1‑140 (MQ=255)                                       gTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGGACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg                                                                                          <  1:289472/140‑1 (MQ=255)                                        tATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggt                                                                                         >  1:294917/1‑140 (MQ=255)                                        tATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggt                                                                                         >  1:239949/1‑140 (MQ=255)                                         aTCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGCTGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                        <  1:881161/140‑1 (MQ=255)                                         aTCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                        <  1:908481/140‑1 (MQ=255)                                         aTCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                        <  1:329065/140‑1 (MQ=255)                                         aTCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                        <  1:582784/140‑1 (MQ=255)                                          tCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc                                                                                       >  1:424551/1‑140 (MQ=255)                                          tCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc                                                                                       <  2:533639/140‑1 (MQ=255)                                           ccACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg                                                                                          <  1:609501/136‑1 (MQ=255)                                           ccACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg                                                                                          >  2:609501/1‑136 (MQ=255)                                               ccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGt                                                                                   >  2:825582/1‑139 (MQ=255)                                               ccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGt                                                                                   <  1:825582/139‑1 (MQ=255)                                                   cagGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                        >  1:385925/1‑130 (MQ=255)                                                   cagGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                        <  2:385925/130‑1 (MQ=255)                                                      gtggtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACg                                                                           >  1:545743/1‑140 (MQ=255)                                                         gtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGc                                                                        >  1:797594/1‑140 (MQ=255)                                                             ccGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGtt                                                                    >  1:217747/1‑140 (MQ=255)                                                                tCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGcc                                                                 >  2:659358/1‑140 (MQ=255)                                                                    tGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTg                                                                      <  2:676484/131‑1 (MQ=255)                                                                    tGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTg                                                                      >  1:676484/1‑131 (MQ=255)                                                                    tGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTg                                                             >  2:847570/1‑140 (MQ=255)                                                                    tGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTg                                                             <  1:586468/140‑1 (MQ=255)                                                                    tGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTg                                                             >  1:396731/1‑140 (MQ=255)                                                                        tCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGTCGTTGACTc                                                         <  2:405743/140‑1 (MQ=255)                                                                        tCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTc                                                         <  1:522140/140‑1 (MQ=255)                                                                        tCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTc                                                         <  1:327711/140‑1 (MQ=255)                                                                        tCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTc                                                         <  2:239949/140‑1 (MQ=255)                                                                                   gTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTg                                              >  1:35037/1‑140 (MQ=255)                                                                                     tCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCg                                            <  2:675459/140‑1 (MQ=255)                                                                                     tCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCg                                            <  2:424551/140‑1 (MQ=255)                                                                                     tCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCg                                            <  2:306984/140‑1 (MQ=255)                                                                                     tCCTGGAACACGATGACGCCAACCGTGCATTGATGGGAGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCg                                            >  2:471606/1‑140 (MQ=255)                                                                                          gAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGc                                       >  1:421777/1‑140 (MQ=255)                                                                                           aaCACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCt                                      <  2:732133/140‑1 (MQ=255)                                                                                              acGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTaaa                                   <  1:471606/140‑1 (MQ=255)                                                                                                gATGACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACg                                 >  2:798796/1‑140 (MQ=255)                                                                                                   gACGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg                              <  2:454051/140‑1 (MQ=255)                                                                                                     cGCCAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtggtg                            <  1:659358/140‑1 (MQ=255)                                                                                                       ccAACCGTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtggtggt                          <  2:457641/140‑1 (MQ=255)                                                                                                             gTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGGCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGtt                    <  1:597104/140‑1 (MQ=255)                                                                                                             gTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGtt                    <  2:35037/140‑1 (MQ=255)                                                                                                             gTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGtt                    <  1:218105/140‑1 (MQ=255)                                                                                                             gTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGtt                    <  1:163083/140‑1 (MQ=255)                                                                                                             gTGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGtt                    <  2:421777/140‑1 (MQ=255)                                                                                                              tGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTc                   >  2:220884/1‑140 (MQ=255)                                                                                                              tGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTc                   >  1:585707/1‑140 (MQ=255)                                                                                                              tGCATTGATGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTc                   >  2:908197/1‑140 (MQ=255)                                                                                                                     aTGGGTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGt            >  2:177854/1‑140 (MQ=255)                                                                                                                         gTGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGAt        <  1:798796/140‑1 (MQ=255)                                                                                                                          tGCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATg       >  1:152150/1‑140 (MQ=255)                                                                                                                           gCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCTGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGc      >  2:111935/1‑140 (MQ=255)                                                                                                                           gCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGc      <  2:152150/140‑1 (MQ=255)                                                                                                                           gCGAACATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGc      <  1:847570/140‑1 (MQ=255)                                                                                                                               aCATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGAt        <  2:1007861/134‑1 (MQ=255)                                                                                                                               aCATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGAt        >  1:1007861/1‑134 (MQ=255)                                                                                                                               aCATGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTcc  >  1:43177/1‑140 (MQ=255)                                                                                                                                 aTGCGACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGc      >  2:712158/1‑134 (MQ=255)                                                                                                                                 aTGCGACGTCAGGCCGTTCCCACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGc      <  1:712158/134‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      CCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCC  >  NC_000913/4183169‑4183434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑