Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,222,887	T→C	T248A (ACC→GCC)	iclR ←	DNA‑binding transcriptional repressor IclR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,222,887	0	T	C	100.0%	326.6 / NA	91	T248A (ACC→GCC)	iclR	DNA‑binding transcriptional repressor IclR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/45);  total (46/45)

TTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGT  >  NC_000913/4222753‑4223019                                                                                                                                       |                                                                                                                                     ttGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAAt                                                                                                                                   >  1:405392/1‑138 (MQ=255) ttGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAAt                                                                                                                                   <  2:405392/138‑1 (MQ=255) ttGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATAc                                                                                                                                 >  1:624886/1‑140 (MQ=255) ttGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATAc                                                                                                                                 >  2:934539/1‑140 (MQ=255) ttGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATAc                                                                                                                                 >  1:812259/1‑140 (MQ=255) ttGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATAc                                                                                                                                 >  1:952923/1‑140 (MQ=255) ttGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATAc                                                                                                                                 >  1:292441/1‑140 (MQ=255)   gCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGt                                                                                                                               >  2:77763/1‑140 (MQ=255)      aCTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGaa                                                                                                                            >  2:957902/1‑140 (MQ=255)       cTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGaaa                                                                                                                           >  2:85474/1‑140 (MQ=255)       cTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGaaa                                                                                                                           <  1:450546/140‑1 (MQ=255)            gTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGt                                                                                                                      >  2:841178/1‑140 (MQ=255)             tATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTc                                                                                                                     <  1:551574/140‑1 (MQ=255)                  tgGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTc                                                                                                                     <  2:787865/135‑1 (MQ=255)                  tgGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTc                                                                                                                     >  1:787865/1‑135 (MQ=255)                  tgGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGaa                                                                                                                >  2:515021/1‑140 (MQ=255)                    ggCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAAt                                                                                                              >  2:248813/1‑140 (MQ=255)                    ggCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAAt                                                                                                              >  2:243963/1‑140 (MQ=255)                    ggCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAAt                                                                                                              >  2:606670/1‑140 (MQ=255)                      cAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAg                                                                                                            >  2:315759/1‑140 (MQ=255)                      cAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAg                                                                                                            >  1:919033/1‑140 (MQ=255)                      cAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAg                                                                                                            >  2:48387/1‑140 (MQ=255)                      cAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAg                                                                                                            >  2:588144/1‑140 (MQ=255)                       aGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGa                                                                                                           >  2:21482/1‑140 (MQ=255)                              tGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCg                                                                                                    <  1:534449/140‑1 (MQ=255)                                 ctctGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCa                                                                                                 <  1:77763/140‑1 (MQ=255)                                  tctGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCTGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCaa                                                                                                <  2:71316/140‑1 (MQ=255)                                  tctGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCaa                                                                                                <  2:205381/140‑1 (MQ=255)                                   ctGCCCGCCAGAAAAAGTCAGCGCATTCCATCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCaaa                                                                                               <  2:624886/140‑1 (MQ=255)                                     gCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACg                                                                                             <  1:841178/140‑1 (MQ=255)                                     gCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACg                                                                                             >  1:858986/1‑140 (MQ=255)                                     gCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACg                                                                                             >  2:281027/1‑140 (MQ=255)                                     gCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCAGGAAATAGAAATTGCGGCAAACg                                                                                             >  1:938031/1‑140 (MQ=255)                                           cAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCAc                                                                                       <  1:588144/140‑1 (MQ=255)                                            aGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACg                                                                                      <  2:858986/140‑1 (MQ=255)                                               aaaaGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTg                                                                                   <  1:239061/140‑1 (MQ=255)                                                  aGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTc                                                                                <  1:515021/140‑1 (MQ=255)                                                    tCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCAt                                                                              <  2:68321/140‑1 (MQ=255)                                                    tCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCAt                                                                              <  1:989305/140‑1 (MQ=255)                                                     cAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATc                                                                             >  2:489420/1‑140 (MQ=255)                                                     cAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATc                                                                             >  1:769796/1‑140 (MQ=255)                                                     cAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATc                                                                             >  1:936131/1‑140 (MQ=255)                                                       gcgcATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGa                                                                           <  2:812305/140‑1 (MQ=255)                                                       gcgcATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGa                                                                           <  1:957902/140‑1 (MQ=255)                                                        cgcATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGaa                                                                          >  2:823777/1‑140 (MQ=255)                                                        cgcATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGaa                                                                          >  2:820841/1‑140 (MQ=255)                                                        cgcATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGaa                                                                          >  1:43038/1‑140 (MQ=255)                                                             tCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATac                                                                     >  1:237434/1‑140 (MQ=255)                                                              ccACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATaca                                                                    <  1:111846/140‑1 (MQ=255)                                                              ccACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATaca                                                                    <  2:110449/140‑1 (MQ=255)                                                              ccACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATaca                                                                    <  1:21482/140‑1 (MQ=255)                                                              ccACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATaca                                                                    <  1:823777/140‑1 (MQ=255)                                                                   gTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTg                                                               <  1:489420/140‑1 (MQ=255)                                                                     aCGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCa                                                             >  2:131118/1‑140 (MQ=255)                                                                        ccAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAgg                                                          <  2:919033/140‑1 (MQ=255)                                                                         cAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGc                                                         >  1:169838/1‑140 (MQ=255)                                                                         cAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGc                                                         >  1:625495/1‑140 (MQ=255)                                                                              tCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGt                                                    <  2:169838/140‑1 (MQ=255)                                                                              tCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGt                                                    <  2:952923/140‑1 (MQ=255)                                                                              tCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGt                                                    >  1:41493/1‑140 (MQ=255)                                                                                 cttccttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGc                                                 >  2:973860/1‑140 (MQ=255)                                                                                 cttccttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGc                                                 <  1:462512/140‑1 (MQ=255)                                                                                   tccttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCGAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGccc                                               <  1:243963/140‑1 (MQ=255)                                                                                   tccttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGccc                                               <  2:987301/140‑1 (MQ=255)                                                                                    ccttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGcccc                                              <  2:237434/140‑1 (MQ=255)                                                                                    ccttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGcccc                                              <  1:281027/140‑1 (MQ=255)                                                                                    ccttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGcccc                                              <  2:769796/140‑1 (MQ=255)                                                                                    ccttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGcccc                                              <  1:934539/140‑1 (MQ=255)                                                                                      ttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCa                                             >  2:105854/1‑139 (MQ=255)                                                                                      ttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCa                                             <  1:105854/139‑1 (MQ=255)                                                                                      ttcGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAg                                            <  2:905085/140‑1 (MQ=255)                                                                                           cgcTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGccc                                               >  2:381143/1‑132 (MQ=255)                                                                                           cgcTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGccc                                               <  1:381143/132‑1 (MQ=255)                                                                                                       cATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGt                           >  2:935046/1‑140 (MQ=255)                                                                                                       cATCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGt                           >  2:404419/1‑140 (MQ=255)                                                                                                        aTCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTc                          >  1:522430/1‑140 (MQ=255)                                                                                                        aTCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTc                          <  1:925624/140‑1 (MQ=255)                                                                                                        aTCGCGCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTc                          <  2:522430/140‑1 (MQ=255)                                                                                                          cgcgCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCaa                        <  1:404419/140‑1 (MQ=255)                                                                                                          cgcgCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCaa                        <  1:315759/140‑1 (MQ=255)                                                                                                          cgcgCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCaa                        <  1:305678/140‑1 (MQ=255)                                                                                                          cgcgCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCaa                        >  1:418424/1‑140 (MQ=255)                                                                                                          cgcgCCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCaa                        >  1:989966/1‑140 (MQ=255)                                                                                                            cgcCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCt                                 <  1:673944/129‑1 (MQ=255)                                                                                                            cgcCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCt                                 >  2:673944/1‑129 (MQ=255)                                                                                                            cgcCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCAAAt                      >  2:254054/1‑140 (MQ=255)                                                                                                            cgcCAAACTCGGTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCAAAt                      <  2:41493/140‑1 (MQ=255)                                                                                                                       gTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCAAATGAATAAcccc            <  1:570318/139‑1 (MQ=255)                                                                                                                       gTCACGCGGTCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCAAATGAATAAcccc            >  2:570318/1‑139 (MQ=255)                                                                                                                                tCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGt  <  1:935046/140‑1 (MQ=255)                                                                                                                                tCATCGGCAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGt  >  1:871192/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     TTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGT  >  NC_000913/4222753‑4223019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑