Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,296,578	T→C	Y183C (TAC→TGC)	yjcO ←	Sel1 repeat‑containing protein YjcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,296,578	0	T	C	100.0%	88.1 / NA	26	Y183C (TAC→TGC)	yjcO	Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/14);  total (12/14)

TTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATCAAACCAAGCAGCATTTGGGCATCGACGGCAGAGTCG  >  NC_000913/4296451‑4296712                                                                                                                                |                                                                                                                                       tttttCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAATCCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAAc                                                                                                                            <  1:825460/140‑1 (MQ=255) tttttCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAAc                                                                                                                            <  1:445931/140‑1 (MQ=255) tttttCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAAc                                                                                                                            <  1:490227/140‑1 (MQ=255)        aaCTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCg                                                                                                                     <  1:618600/140‑1 (MQ=255)               cacaCCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAAtt                                                                                                              >  1:987581/1‑140 (MQ=255)                     cGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCagag                                                                                                        >  1:776524/1‑140 (MQ=255)                      ggTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGc                                                                                                       <  1:118849/140‑1 (MQ=255)                            aaaCCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGtt                                                                                                 <  2:53740/140‑1 (MQ=255)                                  ttCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTg                                                                                                     >  2:282073/1‑130 (MQ=255)                                  ttCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTg                                                                                                     <  1:282073/130‑1 (MQ=255)                                  ttCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAAt                                                                                           >  1:161844/1‑140 (MQ=255)                                    ccATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATAc                                                                                         >  1:928806/1‑140 (MQ=255)                                     cATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATAcc                                                                                        <  2:912610/140‑1 (MQ=255)                                           gCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTg                                                                                  <  2:928806/140‑1 (MQ=255)                                            cTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGc                                                                                 <  2:776524/140‑1 (MQ=255)                                            cTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGc                                                                                 >  1:298238/1‑140 (MQ=255)                                             tCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATTCCAGGTTGcc                                                                                <  2:161844/140‑1 (MQ=255)                                                       ccAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAtcgt                                                                      <  2:822605/140‑1 (MQ=255)                                                           tGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGc                                                                  >  1:831979/1‑140 (MQ=255)                                                                                                      cGTTTAAGAACATCATTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATCAAACCAAGCAGCAtt                       >  1:150887/1‑140 (MQ=255)                                                                                                                  tcatTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATCAAACCAAGCAGCATTTGGGCATCGACg           >  2:675622/1‑140 (MQ=255)                                                                                                                   catTCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATCAAACCAAGCAGCATTTGGGCATCGACgg          >  1:76930/1‑140 (MQ=255)                                                                                                                     ttCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATCAAACCAAGCAGCATTTGGGCATCGACGGCa        <  1:675622/140‑1 (MQ=255)                                                                                                                     ttCCCGCCCAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATCAAACCAAGCAGCATTTGGGCATCGACGGCa        >  1:262271/1‑140 (MQ=255)                                                                                                                           cccAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATCAAACCAAGCAGCATTTGGGCATCGACGGCAGAGTCg  >  2:211760/1‑140 (MQ=255)                                                                                                                           cccAGCACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATCAAACCAAGCAGCATTTGGGCATCGACGGCAGAGTCg  <  2:695154/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                       TTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATCAAACCAAGCAGCATTTGGGCATCGACGGCAGAGTCG  >  NC_000913/4296451‑4296712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑