Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,326,835	A→G	intergenic (‑101/‑300)	yjdM ← / → crfC	conserved protein YjdM/clamp‑binding sister replication fork colocalization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,326,835	0	A	G	100.0%	217.2 / NA	65	intergenic (‑101/‑300)	yjdM/crfC	conserved protein YjdM/clamp‑binding sister replication fork colocalization protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/32);  total (33/32)

TTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTACTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTT  >  NC_000913/4326699‑4326967                                                                                                                                         |                                                                                                                                     ttCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGctg                                                                                                                                   >  1:705098/1‑140 (MQ=255) ttCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGctg                                                                                                                                   >  2:459240/1‑140 (MQ=255)  tCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGtgctgc                                                                                                                                  <  1:353797/140‑1 (MQ=255)   cGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTgctgct                                                                                                                                 <  2:539797/140‑1 (MQ=255)    ggAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGctgctg                                                                                                                                >  2:37637/1‑140 (MQ=255)      aGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGAt                                                                                                                              >  1:874778/1‑140 (MQ=255)      aGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGAt                                                                                                                              >  1:160930/1‑140 (MQ=255)       gTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGAtt                                                                                                                             >  2:914231/1‑140 (MQ=255)        ttGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGAttt                                                                                                                            <  2:7695/140‑1 (MQ=255)         tGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGAtttt                                                                                                                           >  1:73106/1‑140 (MQ=255)         tGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGAtttt                                                                                                                           >  2:294747/1‑140 (MQ=255)          gCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTa                                                                                                                          <  1:758750/140‑1 (MQ=255)           cATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAg                                                                                                                         >  2:560391/1‑140 (MQ=255)                  ggCAGTGTGGTAATGACATGTGGATTTCTTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGAcgacga                                                                                                                  <  1:550921/140‑1 (MQ=255)                     aGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCg                                                                                                               >  2:440480/1‑140 (MQ=255)                       tgtgGTAATGACATGTGGATTTCCTCGAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGtt                                                                                                             >  2:844938/1‑140 (MQ=255)                            tAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCAt                                                                                                        <  2:73106/140‑1 (MQ=255)                            tAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCAt                                                                                                        >  1:647658/1‑140 (MQ=255)                                 aCATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGaa                                                                                                   >  2:385143/1‑140 (MQ=255)                                 aCATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGaa                                                                                                   >  2:228321/1‑140 (MQ=255)                                   aTGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAg                                                                                                 >  1:18629/1‑140 (MQ=255)                                   aTGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAg                                                                                                 >  1:846940/1‑140 (MQ=255)                                     gtgGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAATGa                                                                                               >  1:586024/1‑140 (MQ=255)                                     gtgGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGa                                                                                               >  1:700867/1‑140 (MQ=255)                                     gtgGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGa                                                                                               >  2:81938/1‑140 (MQ=255)                                      tgGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGaa                                                                                              >  2:708401/1‑140 (MQ=255)                                      cggAGTTCCTCAAAAGGCAGCAGGGCAAGAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGAGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGaa                                                                                              >  2:754392/2‑140 (MQ=255)                                              cTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCaaaa                                                                                      <  2:874778/140‑1 (MQ=255)                                                 aaaaGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGa                                                                                   >  2:537531/1‑140 (MQ=255)                                                      tCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAaata                                                                              <  2:647658/140‑1 (MQ=255)                                                        agcagGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGAGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAaataat                                                                            >  1:1011438/1‑140 (MQ=255)                                                             ggCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCtttt                                                                       <  2:846940/140‑1 (MQ=255)                                                               cAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTg                                                                     >  1:240102/1‑140 (MQ=255)                                                               cAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTg                                                                     >  2:591522/1‑140 (MQ=255)                                                               cAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTg                                                                     >  1:753792/1‑140 (MQ=255)                                                                aaaaGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGc                                                                    <  1:81938/140‑1 (MQ=255)                                                                aaaaGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGc                                                                    <  1:798995/140‑1 (MQ=255)                                                                 aaaGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGcc                                                                   <  2:1785/140‑1 (MQ=255)                                                                 aaaGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGcc                                                                   <  2:160930/140‑1 (MQ=255)                                                                 aaaGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGcc                                                                   <  1:557509/140‑1 (MQ=255)                                                                 aaaGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGcc                                                                   <  1:914231/140‑1 (MQ=255)                                                                  aaGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCct                                                                  <  2:317431/140‑1 (MQ=255)                                                                       ccAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGc                                                                    >  1:551037/1‑133 (MQ=255)                                                                       ccAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGc                                                                    <  2:551037/133‑1 (MQ=255)                                                                        cAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCtattt                                                            <  1:708401/140‑1 (MQ=255)                                                                         aaaaaaCGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCtattta                                                           <  1:239004/140‑1 (MQ=255)                                                                         aaaaaaCGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCtattta                                                           <  1:228321/140‑1 (MQ=255)                                                                              aCGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTattatt                                                      <  2:26440/140‑1 (MQ=255)                                                                                   aGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGa                                                 <  2:1011438/140‑1 (MQ=255)                                                                                    gATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGAt                                                <  1:560391/140‑1 (MQ=255)                                                                                     aTTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATa                                               >  1:478970/1‑140 (MQ=255)                                                                                         ccGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGt                                           <  1:537531/140‑1 (MQ=255)                                                                                           gAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTa                                         <  1:591522/140‑1 (MQ=255)                                                                                               aaGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGc                                     <  2:240102/140‑1 (MQ=255)                                                                                               aaGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGc                                     >  1:69220/1‑140 (MQ=255)                                                                                                            taCACAAAATCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCtt                        >  2:823592/1‑140 (MQ=255)                                                                                                                   aaTCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCTTTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTaa                 >  1:763025/1‑140 (MQ=255)                                                                                                                   aaTCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTaa                 >  1:1015408/1‑140 (MQ=255)                                                                                                                   aaTCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTaa                 >  1:670459/1‑140 (MQ=255)                                                                                                                    aTCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAATGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAAt                <  2:586024/140‑1 (MQ=255)                                                                                                                     tCCCTGAGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAAtt               <  1:484733/140‑1 (MQ=255)                                                                                                                      cccTGAGTTTGTCGGGGGTGCTGATGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTc              >  2:513503/1‑140 (MQ=255)                                                                                                                           aGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCTTTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATg         <  2:763025/140‑1 (MQ=255)                                                                                                                           aGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATg         <  2:478970/140‑1 (MQ=255)                                                                                                                           aGTTTGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATg         <  1:191991/140‑1 (MQ=255)                                                                                                                           aGTTTGTCGGGGGTGCTGATGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATg         <  1:513503/140‑1 (MQ=255)                                                                                                                               tGTCGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGatat     <  2:670459/140‑1 (MQ=255)                                                                                                                                  cGGGGGTGCTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATAtttt  <  2:72274/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGGGGGTACTGCTGATTTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTT  >  NC_000913/4326699‑4326967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑