Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	727,838	T→C	M299V (ATG→GTG)	kdpA ←	K(+) transporting P‑type ATPase subunit KdpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	727,838	0	T	C	100.0%	209.5 / NA	59	M299V (ATG→GTG)	kdpA	K(+) transporting P‑type ATPase subunit KdpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

CAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCA  >  NC_000913/727702‑727972                                                                                                                                         |                                                                                                                                       cacgAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCAcaccac                                                                                                                                     <  1:265233/137‑1 (MQ=255) caGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCAcaccac                                                                                                                                     >  2:769278/1‑140 (MQ=255)           ccgTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACg                                                                                                                           >  1:260940/1‑140 (MQ=255)                            aaCAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCGTATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCAc                                                                                                          <  1:269561/140‑1 (MQ=255)                            aaCAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCAc                                                                                                          <  1:657070/140‑1 (MQ=255)                                 gCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACa                                                                                                     >  1:182393/1‑140 (MQ=255)                                 gCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACa                                                                                                     <  1:20216/140‑1 (MQ=255)                                 gCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACa                                                                                                     >  1:284222/1‑140 (MQ=255)                                   tACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATc                                                                                                   <  2:281117/140‑1 (MQ=255)                                      tGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGcc                                                                                                <  1:769278/140‑1 (MQ=255)                                      tGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGcc                                                                                                <  2:315192/140‑1 (MQ=255)                                      tGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGcc                                                                                                <  1:210385/140‑1 (MQ=255)                                         ccAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCcac                                                                                             >  1:325240/1‑140 (MQ=255)                                                          ctctctTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTg                                                                            >  1:27083/1‑140 (MQ=255)                                                           tctctTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTgg                                                                           <  2:325240/140‑1 (MQ=255)                                                                  aCCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACTACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGAt                                                                    <  1:1006100/140‑1 (MQ=255)                                                                    cTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCg                                                                  >  1:699263/1‑140 (MQ=255)                                                                      tCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGcc                                                                <  1:854084/140‑1 (MQ=255)                                                                      tCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGcc                                                                >  1:300843/1‑140 (MQ=255)                                                                       ccATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGcc                                                                <  1:842671/139‑1 (MQ=255)                                                                       ccATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGcc                                                                >  2:842671/1‑139 (MQ=255)                                                                         atatTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCAt                                                             >  1:77481/1‑140 (MQ=255)                                                                             tGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACt                                                         >  2:822035/1‑140 (MQ=255)                                                                              gATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACtt                                                        >  1:11670/1‑140 (MQ=255)                                                                               aTGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGATCTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCGGGCGGCGATCGCCCATCACtac                                                       <  2:240819/140‑3 (MQ=255)                                                                                 gctgctGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCAc                                                     >  2:812850/1‑140 (MQ=255)                                                                                  ctgctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCAcc                                                    >  1:859530/1‑140 (MQ=255)                                                                                  ctgctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCAcc                                                    >  2:877513/1‑140 (MQ=255)                                                                                    gctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACt                                                         >  1:555809/1‑133 (MQ=255)                                                                                    gctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACt                                                         <  2:555809/133‑1 (MQ=255)                                                                                    gctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCaa                                                  <  2:699263/140‑1 (MQ=255)                                                                                    gctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCaa                                                  >  1:874978/1‑140 (MQ=255)                                                                                    gctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCaa                                                  >  1:558081/1‑140 (MQ=255)                                                                                    gctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCaa                                                  <  2:300843/140‑1 (MQ=255)                                                                                              cccAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGca                                        <  2:11670/140‑1 (MQ=255)                                                                                                 aGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCa                                             <  1:834445/132‑1 (MQ=255)                                                                                                 aGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCa                                             >  2:834445/1‑132 (MQ=255)                                                                                                 aGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAg                                     >  1:308866/1‑140 (MQ=255)                                                                                                   tGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAgcg                                   <  2:284222/140‑1 (MQ=255)                                                                                                   tGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAgcg                                   <  2:182393/140‑1 (MQ=255)                                                                                                   tGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAgcg                                   >  2:797326/1‑140 (MQ=255)                                                                                                       agcagATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGt                               <  2:77481/140‑1 (MQ=255)                                                                                                        gcagATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGtt                              >  2:410443/1‑140 (MQ=255)                                                                                                           gATGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTggg                           <  1:797326/140‑1 (MQ=255)                                                                                                             tGAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGAt                         >  1:346015/1‑140 (MQ=255)                                                                                                              gAGGATTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAGCATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATc                        >  2:253647/1‑140 (MQ=255)                                                                                                                  aTTACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCaaga                    <  1:812850/140‑1 (MQ=255)                                                                                                                    tACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCaagaag                  >  1:587572/1‑140 (MQ=255)                                                                                                                    tACCCTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCaagaag                  >  1:371380/1‑140 (MQ=255)                                                                                                                      cccTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGAt                >  1:88625/1‑140 (MQ=255)                                                                                                                      cccTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCACACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTggg                           >  2:829973/1‑129 (MQ=255)                                                                                                                      cccTGAACTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCACACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTggg                           <  1:829973/129‑1 (MQ=255)                                                                                                                           aaCTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGcc            >  2:529879/1‑139 (MQ=255)                                                                                                                           aaCTTCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGcc            <  1:529879/139‑1 (MQ=255)                                                                                                                               tCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCAt       <  2:27083/140‑1 (MQ=255)                                                                                                                               tCTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCAt       <  1:822035/140‑1 (MQ=255)                                                                                                                                cTGCCCACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATc      >  2:870680/1‑140 (MQ=255)                                                                                                                                    ccACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCa  <  2:558081/140‑1 (MQ=255)                                                                                                                                    ccACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCa  <  2:365446/140‑1 (MQ=255)                                                                                                                                    ccACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCa  <  2:346015/140‑1 (MQ=255)                                                                                                                                    ccACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCa  <  1:870680/140‑1 (MQ=255)                                                                                                                                    ccACACCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCa  <  2:874978/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCA  >  NC_000913/727702‑727972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑