Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,468,660	G→A	V346I (GTA→ATA)	mgtA →	Mg(2(+)) importing P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,468,660	0	G	A	100.0%	209.1 / NA	59	V346I (GTA→ATA)	mgtA	Mg(2(+)) importing P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/33);  total (26/33)

CCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATC  >  NC_000913/4468525‑4468786                                                                                                                                        |                                                                                                                               ccGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATaaa                                                                                                                            <  2:225121/140‑1 (MQ=255)  cGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAg                                                                                                                           >  1:136034/1‑140 (MQ=255)       gtgCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTc                                                                                                                      <  1:396935/140‑1 (MQ=255)       gtgCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTc                                                                                                                      <  2:72966/140‑1 (MQ=255)       gtgCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTc                                                                                                                      >  1:625112/1‑140 (MQ=255)          cTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGaa                                                                                                                   >  1:80586/1‑140 (MQ=255)            gTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGa                                                                                                                    <  2:703609/137‑1 (MQ=255)            gTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGa                                                                                                                    >  1:703609/1‑137 (MQ=255)              tAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAg                                                                                                               >  2:982970/1‑140 (MQ=255)                 tCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGaaa                                                                                                            <  1:979669/140‑1 (MQ=255)                     tGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAGATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGa                                                                                                        >  1:340258/1‑140 (MQ=255)                     tGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGa                                                                                                        <  2:136034/140‑1 (MQ=255)                      ggTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGAt                                                                                                       <  1:881733/140‑1 (MQ=255)                       gTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATc                                                                                                      >  1:808702/1‑140 (MQ=255)                       gTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATc                                                                                                      >  1:228145/1‑140 (MQ=255)                           cacCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCa                                                                                                  <  1:875509/140‑1 (MQ=255)                           cacCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAAAAAAGCTGTCGAAACAGAAAGTGATCGTCa                                                                                                  <  1:741408/140‑1 (MQ=255)                                 aGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGAt                                                                                                       >  1:917156/1‑129 (MQ=255)                                 aGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGAt                                                                                                       <  2:917156/129‑1 (MQ=255)                                  ggCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCt                                                                                           <  2:808702/140‑1 (MQ=255)                                      aCTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTc                                                                                                   <  2:435879/128‑1 (MQ=255)                                      aCTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTc                                                                                                   >  1:435879/1‑128 (MQ=255)                                      aCTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCt                                                                                           <  1:618072/136‑1 (MQ=255)                                      aCTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCt                                                                                           >  2:618072/1‑136 (MQ=255)                                            gggAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTAtt                                                                                 >  1:885676/1‑140 (MQ=255)                                                    gcgcTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACtt                                                                         >  2:570461/1‑140 (MQ=255)                                                     cgcTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACttt                                                                        >  1:459007/1‑140 (MQ=255)                                                       cTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGTAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTgg                                                                      <  1:774621/140‑1 (MQ=255)                                                          tttGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAGATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc                                                                   <  2:340258/140‑1 (MQ=255)                                                          tttGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAg                                                                              >  2:969934/1‑129 (MQ=255)                                                          tttGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAg                                                                              <  1:969934/129‑1 (MQ=255)                                                          tttGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc                                                                   <  1:79407/140‑1 (MQ=255)                                                          tttGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc                                                                   <  1:722604/140‑1 (MQ=255)                                                          tttGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc                                                                   <  2:80586/140‑1 (MQ=255)                                                          tttGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc                                                                   <  1:570461/140‑1 (MQ=255)                                                          tttGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc                                                                   <  1:451477/140‑1 (MQ=255)                                                          tttGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc                                                                   <  1:834843/140‑1 (MQ=255)                                                               gcTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATgg                                                              <  2:376148/140‑1 (MQ=255)                                                                 tttCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGat                                                            <  2:459007/140‑1 (MQ=255)                                                                           ggTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCa                                                  <  1:982970/140‑1 (MQ=255)                                                                                     aCGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCTGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAAc                                        >  1:43867/1‑140 (MQ=255)                                                                                     aCGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAAc                                        >  1:15893/1‑140 (MQ=255)                                                                                        ccGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCgg                                     <  2:885676/140‑1 (MQ=255)                                                                                           gAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCAc                                  >  1:1001493/1‑140 (MQ=255)                                                                                                 ttGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGAc                            >  2:494983/1‑140 (MQ=255)                                                                                                  tGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCTGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACg                           <  2:43867/140‑1 (MQ=255)                                                                                                  tGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACg                           <  1:853719/140‑1 (MQ=255)                                                                                                    ccGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCa                         >  2:786142/1‑140 (MQ=255)                                                                                                              ttACCTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAAtt               >  2:843015/1‑140 (MQ=255)                                                                                                                 ccTCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATtgtg            <  1:786142/140‑1 (MQ=255)                                                                                                                   tCGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCt          >  1:885186/1‑140 (MQ=255)                                                                                                                    cGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTg         >  2:377855/1‑140 (MQ=255)                                                                                                                    cGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTg         <  2:287346/140‑1 (MQ=255)                                                                                                                    cGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTg         <  1:225038/140‑1 (MQ=255)                                                                                                                    cGACGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTg         <  2:885186/140‑1 (MQ=255)                                                                                                                      aCGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGga       >  1:135700/1‑140 (MQ=255)                                                                                                                      aCGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGga       >  2:981462/1‑140 (MQ=255)                                                                                                                      aCGCTGGCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGga       >  2:789222/1‑140 (MQ=255)                                                                                                                           ggCGCGCGGCGCAATAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATc  <  1:843015/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                               CCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTTTGCGCTTTCGGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATC  >  NC_000913/4468525‑4468786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑