Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	748,647	T→C	G112G (GGA→GGG)	ybgO ←	putative fimbrial protein YbgO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	748,647	0	T	C	100.0%	258.3 / NA	72	G112G (GGA→GGG)	ybgO	putative fimbrial protein YbgO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/31);  total (41/31)

GGTACGCGACATATGAATATTAGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGT  >  NC_000913/748512‑748777                                                                                                                                        |                                                                                                                                   ggTACGCGACATATGAATATTAGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCa                                                                                                                                <  2:243223/140‑1 (MQ=255)       cgACATATGAATATTAGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTgg                                                                                                                          >  1:469924/1‑140 (MQ=255)           atatGAATATTAGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATc                                                                                                                      <  1:194437/140‑1 (MQ=255)            tatGAATATTAGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCg                                                                                                                     >  1:882696/1‑140 (MQ=255)                  tatTAGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTTGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCg                                                                                                               >  1:508060/1‑140 (MQ=255)                     tAGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGaca                                                                                                            >  1:212208/1‑140 (MQ=255)                     tAGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGaca                                                                                                            <  1:71608/140‑1 (MQ=255)                      aGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGaca                                                                                                            <  1:913788/139‑1 (MQ=255)                      aGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGaca                                                                                                            >  2:913788/1‑139 (MQ=255)                       gCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGa                                                                                                              >  1:319464/1‑136 (MQ=255)                       gCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGa                                                                                                              <  2:319464/136‑1 (MQ=255)                       gCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACAcc                                                                                                          >  2:273592/1‑140 (MQ=255)                       gCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACAcc                                                                                                          >  1:546693/1‑140 (MQ=255)                       gCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGACATCGTAATCGACAcc                                                                                                          >  2:489945/1‑140 (MQ=255)                        cGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCg                                                                                                         >  1:334812/1‑140 (MQ=255)                        cGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCg                                                                                                         >  2:750735/1‑140 (MQ=255)                          ggATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGtt                                                                                                       <  1:273592/140‑1 (MQ=255)                            aTCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGt                                                                                                     >  1:991637/1‑140 (MQ=255)                               gCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGaca                                                                                                            >  2:598440/1‑130 (MQ=255)                               gCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGaca                                                                                                            <  1:598440/130‑1 (MQ=255)                                cccGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGt                                                                                                 >  2:615228/1‑140 (MQ=255)                                    aGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACg                                                                                             <  2:469924/140‑1 (MQ=255)                                    aGCAAATTTTCTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACg                                                                                             <  2:882696/140‑1 (MQ=255)                                     gCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGc                                                                                            >  2:245811/1‑140 (MQ=255)                                      cAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGACATCGTAATCGACACCGTTGTATGTCACGcc                                                                                           <  1:489945/140‑1 (MQ=255)                                              gTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGat                                                                                   <  1:615228/140‑1 (MQ=255)                                                           atcGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTg                                                                      <  2:212208/140‑1 (MQ=255)                                                           atcGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTg                                                                      >  2:63437/1‑140 (MQ=255)                                                             cgcgATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCa                                                                    <  1:750735/140‑1 (MQ=255)                                                              gcgATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCAc                                                                   >  1:989573/1‑140 (MQ=255)                                                              gcgATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCAc                                                                   >  1:252184/1‑140 (MQ=255)                                                                gatgatCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCAccc                                                                 <  2:252184/140‑1 (MQ=255)                                                                 atgatCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCAcccc                                                                >  2:472592/1‑140 (MQ=255)                                                                      cTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTTGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGAt                                                           <  2:508060/140‑1 (MQ=255)                                                                         cTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAgg                                                        >  1:140119/1‑140 (MQ=255)                                                                              gggTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGttt                                                   >  1:446393/1‑140 (MQ=255)                                                                                 tataAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                <  2:446393/140‑1 (MQ=255)                                                                                 tataAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                <  2:991637/140‑1 (MQ=255)                                                                                 tataAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                <  2:220103/140‑1 (MQ=255)                                                                                 tataAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                <  1:63437/140‑1 (MQ=255)                                                                                   taAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATa                                                          <  2:146201/128‑1 (MQ=255)                                                                                   taAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATa                                                          >  1:146201/1‑128 (MQ=255)                                                                                   taAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                >  2:180538/1‑138 (MQ=255)                                                                                   taAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                <  1:180538/138‑1 (MQ=255)                                                                                   taAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCa                                              >  2:435656/1‑140 (MQ=255)                                                                                   taAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACACCCGGATAGGGGTTTACCCa                                              >  1:654769/1‑140 (MQ=255)                                                                                        atCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                <  1:363773/133‑1 (MQ=255)                                                                                        atCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                >  2:363773/1‑133 (MQ=255)                                                                                         tCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                <  1:770164/132‑1 (MQ=255)                                                                                         tCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                >  2:770164/1‑132 (MQ=255)                                                                                          cAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAg                                       >  2:328742/1‑140 (MQ=255)                                                                                           aaTGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAccc                                               >  1:216424/1‑131 (MQ=255)                                                                                           aaTGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAccc                                               <  2:216424/131‑1 (MQ=255)                                                                                                 cTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCt                                <  2:140119/140‑1 (MQ=255)                                                                                                                 tGGTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGcc                <  1:820355/140‑1 (MQ=255)                                                                                                                  ggTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGc                 <  2:38509/138‑1 (MQ=255)                                                                                                                  ggTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGc                 >  1:38509/1‑138 (MQ=255)                                                                                                                  ggTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCg               >  1:122969/1‑140 (MQ=255)                                                                                                                  ggTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCg               >  1:286590/1‑140 (MQ=255)                                                                                                                  ggTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCg               >  2:529752/1‑140 (MQ=255)                                                                                                                  ggTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCg               >  1:479047/1‑140 (MQ=255)                                                                                                                  ggTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCg               <  1:529752/140‑1 (MQ=255)                                                                                                                  ggTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCg               >  1:809187/1‑140 (MQ=255)                                                                                                                   gTTAGTGTCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGt              >  2:883904/1‑140 (MQ=255)                                                                                                                          tCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAtt       <  1:435656/140‑1 (MQ=255)                                                                                                                          tCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAtt       >  2:298480/1‑140 (MQ=255)                                                                                                                          tCGATGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAtt       >  2:1012643/1‑140 (MQ=255)                                                                                                                             aTGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTa         <  2:502592/135‑1 (MQ=255)                                                                                                                             aTGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTa         >  1:502592/1‑135 (MQ=255)                                                                                                                             aTGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAttgtt    >  2:477576/1‑140 (MQ=255)                                                                                                                             aTGCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAttgtt    >  2:79057/1‑140 (MQ=255)                                                                                                                               gCGACTTTTCCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATtgttgt  <  2:349877/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   GGTACGCGACATATGAATATTAGCGGGATCGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGTTCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGT  >  NC_000913/748512‑748777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑