Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	184,266	T→C	E268E (GAA→GAG)	yaeI ←	phosphodiesterase YaeI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	184,266	0	T	C	100.0%	159.1 / NA	45	E268E (GAA→GAG)	yaeI	phosphodiesterase YaeI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/20);  total (25/20)

TATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCA  >  NC_000913/184130‑184398                                                                                                                                         |                                                                                                                                     tATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTcc                                                                                                                                   <  1:817530/140‑1 (MQ=255)  aTTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCa                                                                                                                                  <  2:125048/140‑1 (MQ=255)              ggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACtt                                                                                                                      >  1:345660/1‑140 (MQ=255)                         cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                           >  2:84293/1‑140 (MQ=255)                         cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                           >  1:242398/1‑140 (MQ=255)                         cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                           >  2:485058/1‑140 (MQ=255)                         cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                           >  1:565604/1‑140 (MQ=255)                         cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                           >  1:609630/1‑140 (MQ=255)                              cTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAg                                                                                                      >  1:175337/1‑140 (MQ=255)                                   cTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCa                                                                                                 <  2:609630/140‑1 (MQ=255)                                    tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                                <  2:242398/140‑1 (MQ=255)                                                aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                                    <  2:61929/124‑1 (MQ=255)                                                aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                                    >  1:61929/1‑124 (MQ=255)                                                      ttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccac                                                                              <  1:54522/140‑1 (MQ=255)                                                           ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                         <  2:565604/140‑1 (MQ=255)                                                              cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                      <  1:789934/140‑1 (MQ=255)                                                              cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                      <  1:945917/140‑1 (MQ=255)                                                              cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                      <  1:956596/140‑1 (MQ=255)                                                              cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                      <  2:13400/140‑1 (MQ=255)                                                                gACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGtt                                                                    >  2:108040/1‑140 (MQ=255)                                                                     cTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                           <  1:494003/128‑1 (MQ=255)                                                                     cTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                           >  2:494003/1‑128 (MQ=255)                                                                           tGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtgtg                                                         >  1:201759/1‑140 (MQ=255)                                                                             gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtgtg                                                                 >  2:102513/1‑130 (MQ=255)                                                                             gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtgtg                                                                 <  1:102513/130‑1 (MQ=255)                                                                             gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTc                                                       >  2:749536/1‑140 (MQ=255)                                                                             gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTc                                                       >  1:262555/1‑140 (MQ=255)                                                                                     aTCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCa                                               <  1:108040/140‑1 (MQ=255)                                                                                       cAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa                                             <  1:749536/140‑1 (MQ=255)                                                                                          aaaaTTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGc                                          <  1:172029/140‑1 (MQ=255)                                                                                                           tCTGGTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGt                         >  1:414341/1‑140 (MQ=255)                                                                                                               gTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGc                              >  1:849144/1‑131 (MQ=255)                                                                                                               gTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGc                              <  2:849144/131‑1 (MQ=255)                                                                                                               gTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACg                     >  2:986766/1‑140 (MQ=255)                                                                                                               gTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACg                     >  1:90317/1‑140 (MQ=255)                                                                                                               gTGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTGTCGCCAAAGGCATTTAATCCGGCGACGTAACg                     >  2:899048/1‑140 (MQ=255)                                                                                                                tGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt                    <  2:262555/140‑1 (MQ=255)                                                                                                                tGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt                    <  1:84293/140‑1 (MQ=255)                                                                                                                  aTAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGttt                  >  2:570991/1‑140 (MQ=255)                                                                                                                  aTAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGttt                  >  2:716843/1‑140 (MQ=255)                                                                                                                  aTAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt                    <  1:734064/138‑1 (MQ=255)                                                                                                                  aTAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt                    >  2:734064/1‑138 (MQ=255)                                                                                                                    aaaaTTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTAt                >  2:1009999/1‑140 (MQ=255)                                                                                                                         tCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCg           >  2:148328/1‑140 (MQ=255)                                                                                                                                  acacCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCa  >  1:64587/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCA  >  NC_000913/184130‑184398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑