Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	869,831	A→G	T141A (ACA→GCA)	gsiB →	glutathione ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	869,831	0	A	G	100.0%	114.7 / NA	33	T141A (ACA→GCA)	gsiB	glutathione ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/15);  total (18/15)

CATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTGGGAAC  >  NC_000913/869695‑869964                                                                                                                                         |                                                                                                                                      cATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCaa                                                                                                                                    <  2:221167/140‑1 (MQ=255)      aaTTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTa                                                                                                                               <  1:308416/140‑1 (MQ=255)                  gCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGTAACGGTAAAGATTACCCTc                                                                                                                   >  2:479791/1‑140 (MQ=255)                   cACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATGTTAAACGCTATAACCTGTATAAGAATATTGCTGAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCa                                                                                                                  >  2:947730/1‑140 (MQ=255)                   cACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGAt                                                                                                                          <  1:457716/132‑1 (MQ=255)                   cACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGAt                                                                                                                          >  2:457716/1‑132 (MQ=255)                       gATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACa                                                                                                              <  1:896069/140‑1 (MQ=255)                        aTTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGAt                                                                                                                          >  2:173947/1‑127 (MQ=255)                        aTTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGAt                                                                                                                          <  1:173947/127‑1 (MQ=255)                         tttCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGc                                                                                                            <  2:436302/140‑1 (MQ=255)                                  cgcgGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAg                                                                                                   >  1:28073/1‑140 (MQ=255)                                    cggcggTGAAAGCGAATCTGGACTGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCg                                                                                                 <  2:359997/140‑1 (MQ=255)                                    cggcggTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCg                                                                                                 <  2:324689/140‑1 (MQ=255)                                          tGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTttatt                                                                                           <  2:192810/140‑1 (MQ=255)                                                    tCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTg                                                                                 <  1:618665/140‑1 (MQ=255)                                                             ggCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCgg                                                                        >  1:144507/1‑140 (MQ=255)                                                                               tcatcTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCAc                                                      >  2:1009582/1‑140 (MQ=255)                                                                                catcTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCAcc                                                     >  2:504003/1‑140 (MQ=255)                                                                                       aaCGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAgcg                                              <  2:28073/140‑1 (MQ=255)                                                                                       aaCGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCACGATGATTTCACCGGCAgcg                                              >  1:841567/1‑140 (MQ=255)                                                                                           cTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTgg                                          <  2:224428/140‑1 (MQ=255)                                                                                                     tataAGAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATgg                                >  2:780903/1‑140 (MQ=255)                                                                                                          gAATATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAgg                           >  1:288435/1‑140 (MQ=255)                                                                                                           aaTATTGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGga                          >  2:122741/1‑140 (MQ=255)                                                                                                               ttGCTAAAACGGAAGNGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGAtt                      >  1:611545/1‑140 (MQ=255)                                                                                                               ttGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGAtt                      >  1:1012307/1‑140 (MQ=255)                                                                                                               ttGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGAtt                      >  2:449012/1‑140 (MQ=255)                                                                                                               ttGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGAtt                      >  1:138467/1‑140 (MQ=255)                                                                                                               ttGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGAtt                      >  2:781933/1‑140 (MQ=255)                                                                                                               ttGCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTGATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGAtt                      >  2:925431/1‑140 (MQ=255)                                                                                                                 gCTAAAACGGAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTgg                    <  1:560014/140‑1 (MQ=255)                                                                                                                         ggAAGCGATCGATCCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATc            <  1:925431/140‑1 (MQ=255)                                                                                                                                   gatcCGGCAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTGGGAAc  <  1:13630/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTGGGAAC  >  NC_000913/869695‑869964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑