Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	959,905	A→G	Y365C (TAC→TGC)	aroA →	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	959,905	0	A	G	100.0%	246.1 / NA	69	Y365C (TAC→TGC)	aroA	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/39);  total (30/39)

ACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTACATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGG  >  NC_000913/959769‑960039                                                                                                                                         |                                                                                                                                       aCGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCAt                                                                                                                                     <  2:387300/140‑1 (MQ=255)         gTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCa                                                                                                                             <  2:1014778/140‑1 (MQ=255)                       gCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGaaaaa                                                                                                               <  2:532377/140‑1 (MQ=255)                           caccacGCTGCGCAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGa                                                                                                           >  2:423579/1‑140 (MQ=255)                                 gCTGCGCAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTg                                                                                                     >  2:429736/1‑140 (MQ=255)                                  cTGCGCAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGc                                                                                                    <  2:454440/140‑1 (MQ=255)                                     cgcAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCga                                                                                                 >  2:90668/1‑140 (MQ=255)                                     cgcAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCga                                                                                                 >  2:437819/1‑140 (MQ=255)                                     cgcAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCga                                                                                                 >  2:810095/1‑140 (MQ=255)                                     cgcAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCga                                                                                                 >  2:709177/1‑140 (MQ=255)                                     cgcAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCga                                                                                                 >  1:892494/1‑140 (MQ=255)                                       cAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCgaga                                                                                               >  1:991992/1‑140 (MQ=255)                                       cAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCgaga                                                                                               <  2:470530/140‑1 (MQ=255)                                          tatCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcg                                                                                            <  2:991992/140‑1 (MQ=255)                                          tatCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcg                                                                                            <  1:449417/140‑1 (MQ=255)                                            tCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcgcg                                                                                          <  1:429736/140‑1 (MQ=255)                                            tCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcgcg                                                                                          <  1:169848/140‑1 (MQ=255)                                            tCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcgcg                                                                                          <  2:892494/140‑1 (MQ=255)                                            tCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcgcg                                                                                          <  2:241790/140‑1 (MQ=255)                                            tCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcgcg                                                                                          <  1:901958/140‑1 (MQ=255)                                            tCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcgcg                                                                                          <  1:810095/140‑1 (MQ=255)                                            tCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcgcg                                                                                          <  2:565871/140‑1 (MQ=255)                                               ataACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACa                                                                                       >  2:475259/1‑140 (MQ=255)                                                taACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACAt                                                                                      <  1:141712/140‑1 (MQ=255)                                                   cTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACa                                                                                   <  1:709177/140‑1 (MQ=255)                                                    tGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACaa                                                                                  >  1:462296/1‑140 (MQ=255)                                                    tGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACaa                                                                                  >  2:870798/1‑140 (MQ=255)                                                      gCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcgcg                                                                                          >  2:742389/1‑130 (MQ=255)                                                      gCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATcgcg                                                                                          <  1:742389/130‑1 (MQ=255)                                                      gCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATg                                                                                >  2:387075/1‑140 (MQ=255)                                                      gCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATg                                                                                >  2:392682/1‑140 (MQ=255)                                                      gCGGGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATg                                                                                >  2:464644/1‑140 (MQ=255)                                                       cGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGa                                                                               >  2:601325/1‑140 (MQ=255)                                                          gtTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCa                                                                            <  1:989944/140‑1 (MQ=255)                                                          gtTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCa                                                                            <  2:462296/140‑1 (MQ=255)                                                              aaGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCgg                                                                        <  1:90668/140‑1 (MQ=255)                                                               agagACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGa                                                                       <  1:475259/140‑1 (MQ=255)                                                                   aCCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGa                                                                       <  1:385669/136‑1 (MQ=255)                                                                   aCCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGa                                                                       >  2:385669/1‑136 (MQ=255)                                                                     cGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCg                                                                  <  2:817257/139‑1 (MQ=255)                                                                     cGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCg                                                                  >  1:817257/1‑139 (MQ=255)                                                                     cGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGa                                                                 >  2:50592/1‑140 (MQ=255)                                                                        tCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGAtgt                                                              >  2:228531/1‑140 (MQ=255)                                                                        tCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGAtgt                                                              >  2:584974/1‑140 (MQ=255)                                                                            cTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGttt                                                          <  2:77646/140‑1 (MQ=255)                                                                                  gCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCt                                                    <  1:392682/140‑1 (MQ=255)                                                                                     aTGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCtggt                                                 <  1:924112/140‑1 (MQ=255)                                                                                      tGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCtggtg                                                <  2:540458/140‑1 (MQ=255)                                                                                       ggCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCtggtgg                                               >  1:689886/1‑140 (MQ=255)                                                                                         cAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCg                                             <  2:957687/140‑1 (MQ=255)                                                                                            cAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTg                                          <  1:146790/140‑1 (MQ=255)                                                                                            cAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTg                                          <  1:464644/140‑1 (MQ=255)                                                                                               aaCTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCa                                       <  1:601325/140‑1 (MQ=255)                                                                                                 cTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGa                                     <  2:689886/140‑1 (MQ=255)                                                                                                    cGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGtt                                           >  1:442545/1‑131 (MQ=255)                                                                                                    cGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGtt                                           <  2:442545/131‑1 (MQ=255)                                                                                                    cGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATac                                  >  2:41154/1‑140 (MQ=255)                                                                                                            cGGCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGa                          <  2:207995/140‑1 (MQ=255)                                                                                                             ggCGCGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGAc                         <  1:41154/140‑1 (MQ=255)                                                                                                              gcgcGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACg                        >  1:618119/1‑140 (MQ=255)                                                                                                              gcgcGGAAGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACg                        <  1:387075/140‑1 (MQ=255)                                                                                                                    aaGTGGAAGAGGGGCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCtt                  <  1:437819/140‑1 (MQ=255)                                                                                                                              ggggCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAAt        >  1:844080/1‑140 (MQ=255)                                                                                                                              ggggCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAAt        >  1:281506/1‑140 (MQ=255)                                                                                                                              ggggCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAAt        >  2:276428/1‑140 (MQ=255)                                                                                                                               gggCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATg       >  1:183008/1‑140 (MQ=255)                                                                                                                                 gCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCa     <  2:183008/140‑1 (MQ=255)                                                                                                                                 gCACGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCa     <  1:276428/140‑1 (MQ=255)                                                                                                                                   aCGATTGCATTCGTATCACTCCTCCGGAAAAACTGAAGTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAAGGCACg   >  2:899876/1‑140 (MQ=255)                                                                                                                                    cGATTGCATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACgg  >  1:970929/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTAAAGAGACCGATCGCCTGTTTGCGATGGCAACAGAACTGCGTAAAGTCGGCGCGGAAGTGGAAGAGGGGCACGATTACATTCGTATCACTCCTCCGGAAAAACTGAACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGG  >  NC_000913/959769‑960039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑