Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,002,743	A→G	T313A (ACA→GCA)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,002,743	0	A	G	100.0%	212.7 / NA	60	T313A (ACA→GCA)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/32);  total (28/32)

GGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGTGAGAATAAATTTTGAT  >  NC_000913/1002608‑1002874                                                                                                                                        |                                                                                                                                    ggATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCAtt                                                                                                                                 >  2:301285/1‑140 (MQ=255) ggATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCAtt                                                                                                                                 >  1:365803/1‑140 (MQ=255)    ttAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTAcc                                                                                                                              <  1:533202/140‑1 (MQ=255)       gTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGct                                                                                                                           <  1:906358/140‑1 (MQ=255)        ttAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGctc                                                                                                                          <  1:287193/140‑1 (MQ=255)            tGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTa                                                                                                                      <  2:22873/140‑1 (MQ=255)                aaCCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTcc                                                                                                                  >  2:66097/1‑140 (MQ=255)                    tGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCtgat                                                                                                              <  1:367501/140‑1 (MQ=255)                      aGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCtg                                                                                                                >  2:594701/1‑136 (MQ=255)                      aGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCtg                                                                                                                <  1:594701/136‑1 (MQ=255)                       gTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTgatgat                                                                                                           <  1:230274/140‑1 (MQ=255)                          tGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCa                                                                                                        >  1:819615/1‑140 (MQ=255)                            aCGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTACTGATGATGCAGa                                                                                                      >  2:653498/1‑140 (MQ=255)                                  aCCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCtgat                                                                                                              >  2:502237/1‑126 (MQ=255)                                  aCCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCtgat                                                                                                              <  1:502237/126‑1 (MQ=255)                                     acaGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgc                                                                                             <  2:256942/140‑1 (MQ=255)                                           cccAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaat                                                                                       <  1:66097/140‑1 (MQ=255)                                            ccAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaatg                                                                                      <  1:426057/140‑1 (MQ=255)                                            ccAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaatg                                                                                      <  2:365803/140‑1 (MQ=255)                                              aGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgcgcg                                                                                          >  2:147806/1‑134 (MQ=255)                                              aGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgcgcg                                                                                          <  1:147806/134‑1 (MQ=255)                                              aGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgc                                                                                             <  2:400451/131‑1 (MQ=255)                                              aGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaatg                                                                                      <  2:286693/138‑1 (MQ=255)                                              aGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaatg                                                                                      >  1:286693/1‑138 (MQ=255)                                              aGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTACTCCTGATGATGCAGACAGTATcgc                                                                                             >  1:400451/1‑131 (MQ=255)                                                   tCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCGCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCt                                                                               <  2:668303/140‑1 (MQ=255)                                                     cccAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaat                                                                                       >  1:81067/1‑130 (MQ=255)                                                     cccAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaat                                                                                       <  2:81067/130‑1 (MQ=255)                                                     cccAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCATGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTAt                                                                             >  2:971041/1‑140 (MQ=255)                                                        aGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCa                                                                          <  1:301285/140‑1 (MQ=255)                                                          gCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGt                                                                        <  2:114190/140‑1 (MQ=255)                                                             aTTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAg                                                                     >  2:123762/1‑140 (MQ=255)                                                                 aGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACg                                                                 <  2:557467/140‑1 (MQ=255)                                                                  gACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGGTGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGa                                                                <  2:212134/140‑1 (MQ=255)                                                                   acgtcacgtcCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGAc                                                               <  2:431598/140‑1 (MQ=255)                                                                   acgtcacgtcCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGAc                                                               <  2:270207/140‑1 (MQ=255)                                                                        acgtcCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGgtg                                                          >  1:58606/1‑140 (MQ=255)                                                                               ggcgtgggcgtgGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTaccacc                                                   <  1:123762/140‑1 (MQ=255)                                                                                    gggcgtggTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGaaa                                              >  2:861407/1‑140 (MQ=255)                                                                                     ggcgtggTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAAc                                             >  1:477754/1‑140 (MQ=255)                                                                                        gtggtggtgACCGATAGCCAGAACAACATTATTTCCCATGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCgg                                          <  1:971041/140‑1 (MQ=255)                                                                                         tggtggtgACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCggg                                         <  2:477754/140‑1 (MQ=255)                                                                                          ggtggtgACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTa                                                        >  1:528867/1‑124 (MQ=255)                                                                                          ggtggtgACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTa                                                        <  2:528867/124‑1 (MQ=255)                                                                                          ggtggtgACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGc                                        <  1:696930/140‑1 (MQ=255)                                                                                          ggtggtgACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGc                                        >  1:307175/1‑140 (MQ=255)                                                                                          ggtggtgACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGc                                        >  1:306801/1‑140 (MQ=255)                                                                                          ggtggtgACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGc                                        >  1:994773/1‑140 (MQ=255)                                                                                           gtggtgACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCg                                       >  2:466297/1‑140 (MQ=255)                                                                                                             aacaacATTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAAc                     >  1:291749/1‑140 (MQ=255)                                                                                                               caacaTTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACgg                   <  1:789315/140‑1 (MQ=255)                                                                                                                aacaTTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTACTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGt                  <  1:653498/140‑1 (MQ=255)                                                                                                                  caTTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGt                  >  2:17737/1‑138 (MQ=255)                                                                                                                  caTTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGt                  <  1:17737/138‑1 (MQ=255)                                                                                                                  caTTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGTg                 <  1:548737/139‑1 (MQ=255)                                                                                                                  caTTATTTCCCCTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGTg                 >  2:548737/1‑139 (MQ=255)                                                                                                                            ccTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGTGAGAATAAAttt      >  2:33947/1‑140 (MQ=255)                                                                                                                            ccTGCTGGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGTGAGAATAAAttt      >  1:351379/1‑140 (MQ=255)                                                                                                                              tgctgGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGTGAGAATAAATTTTg    >  2:182558/1‑140 (MQ=255)                                                                                                                                ctgGTGGAGCATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGTGAGAATAAATTTTGAt  >  2:109520/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    GGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGCCACGGCAACGGTGAGAATAAATTTTGAT  >  NC_000913/1002608‑1002874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑