Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,003,901 G→A V155M (GTG→ATG)  ycbV → putative fimbrial protein YcbV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,003,9010GA100.0% 162.0 / NA 46V155M (GTG→ATG) ycbVputative fimbrial protein YcbV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/25);  total (21/25)

CGGGCTGGCAATTGCGATTCTGGATGATGCGAAGATATTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATGTGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGTGAATTGCAGGCAGGAG  >  NC_000913/1003768‑1004028
                                                                                                                                     |                                                                                                                               
cGGGCTGGCAATTGCGATTCTGGATGATGCGAAGATATTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGt                                                                                                                           <  1:373121/140‑1 (MQ=255)
      ggCAATTGCGATTCTGGATGATGCGAAGATATTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTa                                                                                                                     <  1:839875/140‑1 (MQ=255)
      ggCAATTGCGATTCTGGATGATGCGAAGATATTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTa                                                                                                                     <  1:300224/140‑1 (MQ=255)
            tGCGATTCTGGATGATGCGAAGATATTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGa                                                                                                               >  2:486191/1‑140 (MQ=255)
                  tCTGGATGATGCGAAGATATTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTa                                                                                                                     <  2:224772/128‑1 (MQ=255)
                  tCTGGATGATGCGAAGATATTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTa                                                                                                                     >  1:224772/1‑128 (MQ=255)
                      gatgatGCGAAGATATTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAgcgc                                                                                                     >  2:451389/1‑140 (MQ=255)
                                  atatTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACt                                                                                         <  1:451389/140‑1 (MQ=255)
                                  atatTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGAAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACt                                                                                         <  2:389837/140‑1 (MQ=255)
                                       gATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCAtt                                                                                    <  1:22142/140‑1 (MQ=255)
                                       gATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCAtt                                                                                    <  2:73173/140‑1 (MQ=255)
                                       gATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCAtt                                                                                    <  2:398950/140‑1 (MQ=255)
                                        aTTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTa                                                                                   <  1:486191/140‑1 (MQ=255)
                                          tCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTAc                                                                                          <  1:560033/131‑1 (MQ=255)
                                          tCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTAc                                                                                          >  2:560033/1‑131 (MQ=255)
                                            cGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGAc                                                                               >  1:753414/1‑140 (MQ=255)
                                              cTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACAttt                                                                                              >  1:223990/1‑123 (MQ=255)
                                              cTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACAttt                                                                                              <  2:223990/123‑1 (MQ=255)
                                              cTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTa                                                                             <  2:259299/140‑1 (MQ=255)
                                              cTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTa                                                                             <  1:129301/140‑1 (MQ=255)
                                              cTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCACGACATTTGTACTTCATTATGACTa                                                                             >  2:115290/1‑140 (MQ=255)
                                                  aCCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATAc                                                                         >  2:268061/1‑140 (MQ=255)
                                                   ccAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTCTGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACg                                                                        <  2:887345/140‑1 (MQ=255)
                                                   ccAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACg                                                                        >  1:30410/1‑140 (MQ=255)
                                                       gCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCACGACATTTGTACTTCATTATGACTAATACGTGGa                                                                    <  1:115290/140‑1 (MQ=255)
                                                                  cTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGgcg                                                         <  2:753414/140‑1 (MQ=255)
                                                                     cAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGaa                                                                   >  2:45997/1‑127 (MQ=255)
                                                                     cAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGaa                                                                   <  1:45997/127‑1 (MQ=255)
                                                                      aGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGctct                                                     >  1:198776/1‑140 (MQ=255)
                                                                                 cGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGcc                                          >  1:45270/1‑140 (MQ=255)
                                                                                    tAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGtt                                       >  2:854581/1‑140 (MQ=255)
                                                                                    tAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGtt                                       >  1:102090/1‑140 (MQ=255)
                                                                                       ggTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTa                                    >  1:308993/1‑140 (MQ=255)
                                                                                           ccATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCgc                                >  1:216170/1‑140 (MQ=255)
                                                                                             aTTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCgctg                              >  1:446671/1‑140 (MQ=255)
                                                                                                   tttttATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCtggtg                        <  2:216170/140‑1 (MQ=255)
                                                                                                    ttttATGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCtggtgg                       <  2:446671/140‑1 (MQ=255)
                                                                                                         tGCGCAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCgggg                  >  2:334540/1‑139 (MQ=255)
                                                                                                          gcgcAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGTg                 <  2:308993/140‑1 (MQ=255)
                                                                                                           cgcAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGTGa                >  2:1018326/1‑140 (MQ=255)
                                                                                                            gcAATTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGGGaa               >  1:96676/1‑140 (MQ=255)
                                                                                                              aaTTGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGTGAAtt             <  2:430243/140‑1 (MQ=255)
                                                                                                                 tGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGTGAATTgca          <  2:498888/140‑1 (MQ=255)
                                                                                                                 tGCGACCTGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGTGAATTgca          <  1:1018326/140‑1 (MQ=255)
                                                                                                                        tGTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGGGGATTGcaggcaggg   >  2:619538/1‑139 (MQ=255)
                                                                                                                         gTCAATAGCGATATGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGTGAATTGCAGGCAGGAg  <  2:102090/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                               
CGGGCTGGCAATTGCGATTCTGGATGATGCGAAGATATTGATTCCGCTTAACCAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGATGTGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGGGTGAATTGCAGGCAGGAG  >  NC_000913/1003768‑1004028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑