Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,950	C→T	D48N (GAT→AAT)	yccJ ←	PF13993 family protein YccJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,950	0	C	T	100.0%	107.6 / NA	31	D48N (GAT→AAT)	yccJ	PF13993 family protein YccJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/12);  total (19/12)

TGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTT  >  NC_000913/1066819‑1067073                                                                                                                                    |                                                                                                                            tGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACctt                                                                                                                     >  1:967455/1‑140 (MQ=255)                      cgcgAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTAGGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACGAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCa                                                                                                >  2:899383/1‑140 (MQ=255)                          agaAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCAtttt                                                                                            >  1:355916/1‑140 (MQ=255)                                         gCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGc                                                                             <  1:226562/140‑1 (MQ=255)                                                    gTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGaaaa                                                                  <  2:9208/140‑1 (MQ=255)                                                               cGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCt                                                       >  1:408553/1‑140 (MQ=255)                                                                    gTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTc                                                  <  1:387557/140‑1 (MQ=255)                                                                     tCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTcc                                                 >  2:611004/1‑140 (MQ=255)                                                                     tCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTcc                                                 <  1:611004/140‑1 (MQ=255)                                                                       tGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGc                                                              >  2:790609/1‑125 (MQ=255)                                                                       tGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGc                                                              <  1:790609/125‑1 (MQ=255)                                                                         ttCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTc                                                  >  2:967626/1‑135 (MQ=255)                                                                         ttCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTc                                                  >  2:642764/1‑135 (MQ=255)                                                                         ttCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTc                                                  <  1:967626/135‑1 (MQ=255)                                                                         ttCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTc                                                  <  1:642764/135‑1 (MQ=255)                                                                          tCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTc                                                  <  1:741405/134‑1 (MQ=255)                                                                          tCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTc                                                  >  2:741405/1‑134 (MQ=255)                                                                           cGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAGATGGCCTCGGCTATTTCCGGCGAc                                           <  1:153390/140‑1 (MQ=255)                                                                              cccAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTa                                        <  1:390568/140‑1 (MQ=255)                                                                                        cGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCagag                              >  2:982952/1‑140 (MQ=255)                                                                                        cGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCagag                              >  2:948304/1‑140 (MQ=255)                                                                                            tCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGc                          <  2:802233/140‑1 (MQ=255)                                                                                                        ttAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGAc              >  2:524232/1‑140 (MQ=255)                                                                                                        ttAGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGAc              >  1:236608/1‑140 (MQ=255)                                                                                                          aGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGt            >  1:112146/1‑140 (MQ=255)                                                                                                          aGCAAAGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGt            >  1:291819/1‑140 (MQ=255)                                                                                                               aGGCTTTGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGtgatga       >  1:561750/1‑140 (MQ=255)                                                                                                                   tttGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCtt   >  1:42146/1‑140 (MQ=255)                                                                                                                    ttGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCttt  >  2:987086/1‑140 (MQ=255)                                                                                                                    ttGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCttt  >  2:135885/1‑140 (MQ=255)                                                                                                                    ttGACTAACACTTCATTACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCttt  <  1:948304/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                            TGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTT  >  NC_000913/1066819‑1067073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑