Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,444	C→T	intergenic (‑77/‑58)	rne ← / → yceQ	ribonuclease E/DUF2655 domain‑containing protein YceQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,444	0	C	T	100.0%	187.9 / NA	55	intergenic (‑77/‑58)	rne/yceQ	ribonuclease E/DUF2655 domain‑containing protein YceQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/22);  total (33/22)

AGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGA  >  NC_000913/1144315‑1144575                                                                                                                                  |                                                                                                                                    aGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTa                                                                                                                           >  1:970754/1‑140 (MQ=255) aGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTa                                                                                                                           >  2:826579/1‑140 (MQ=255) aGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTa                                                                                                                           >  2:336039/1‑140 (MQ=255) aGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTa                                                                                                                           >  2:37413/1‑140 (MQ=255)     cAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCgtgt                                                                                                                              <  2:187649/133‑1 (MQ=255)     cAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCgtgt                                                                                                                              >  1:187649/1‑133 (MQ=255)     cAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAgtcg                                                                                                                       >  1:539368/1‑140 (MQ=255)     cAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAgtcg                                                                                                                       <  1:37413/140‑1 (MQ=255)         gcgcAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAgtcgcgtc                                                                                                                   >  1:411259/1‑140 (MQ=255)              aCTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCaa                                                                                                              >  1:14923/1‑140 (MQ=255)                 ctTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCt                                                                                                           <  2:14923/140‑1 (MQ=255)                   tCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCa                                                                                                         <  1:907211/140‑1 (MQ=255)                   tCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCa                                                                                                         <  1:826579/140‑1 (MQ=255)                         tgAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCa                                                                                                         <  2:733480/134‑1 (MQ=255)                         tgAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCa                                                                                                         >  1:733480/1‑134 (MQ=255)                          gAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTAt                                                                                                  >  1:726594/1‑140 (MQ=255)                                cGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAg                                                                                            >  2:836041/1‑140 (MQ=255)                                          cATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAgg                                                                                  <  2:726594/140‑1 (MQ=255)                                          cATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAgg                                                                                  >  1:806505/1‑140 (MQ=255)                                            ttCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGgcg                                                                                <  2:411259/140‑1 (MQ=255)                                             tCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGgcgc                                                                               <  1:836041/140‑1 (MQ=255)                                              cTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCa                                                                              <  1:633409/140‑1 (MQ=255)                                               ttttCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCTGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCag                                                                             >  1:81330/1‑140 (MQ=255)                                               ttttCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTACCGTCAGCTCAAAGAGGCGCag                                                                             >  1:1012264/1‑140 (MQ=255)                                                    aTCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAgtgt                                                                        >  2:762335/1‑140 (MQ=255)                                                    aTCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAgtgt                                                                        >  2:964012/1‑140 (MQ=255)                                                       gTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCgg                                                                     >  1:640417/1‑140 (MQ=255)                                                                    tattCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTc                                                        >  2:237953/1‑140 (MQ=255)                                                                     attCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCa                                                       <  2:640417/140‑1 (MQ=255)                                                                      ttCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCAt                                                      >  1:686554/1‑140 (MQ=255)                                                                                gacgacTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGaaaaaa                                            >  2:1013080/1‑139 (MQ=255)                                                                                gacgacTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAAc                                            >  2:723011/1‑140 (MQ=255)                                                                                gacgacTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAAc                                            >  1:171193/1‑140 (MQ=255)                                                                                 acgacTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACa                                           >  2:1012589/1‑140 (MQ=255)                                                                                 acgacTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACa                                           >  1:181562/1‑140 (MQ=255)                                                                                 acgacTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACa                                           >  1:216319/1‑140 (MQ=255)                                                                                 acgacTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACa                                           >  1:895/1‑140 (MQ=255)                                                                                    acTAAGCTGCTGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAgcg                                        <  2:81330/140‑1 (MQ=255)                                                                                    acTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAgcg                                        <  1:723011/140‑1 (MQ=255)                                                                                    acTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTACCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAgcg                                        <  2:1012264/140‑1 (MQ=255)                                                                                       aaGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCaa                                     <  2:181562/140‑1 (MQ=255)                                                                                           tGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTAt                                 >  2:861374/1‑140 (MQ=255)                                                                                             cGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCa                               >  1:239393/1‑140 (MQ=255)                                                                                                  aaaGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGGAATTATCAAAGaa                          <  1:1012589/140‑1 (MQ=255)                                                                                                    aGTAACGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGTAATTATCAAAGaaaa                        >  2:268463/1‑139 (MQ=255)                                                                                                       aaCGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGa                     <  2:216319/140‑1 (MQ=255)                                                                                                         cGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACt                   <  2:686554/140‑1 (MQ=255)                                                                                                         cGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACt                   <  1:237953/140‑1 (MQ=255)                                                                                                         cGCCTTTCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACt                   <  1:1123/140‑1 (MQ=255)                                                                                                              ttCCGGGTGTGAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTa              >  2:763886/1‑140 (MQ=255)                                                                                                                      gtgAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACtctc      >  2:136436/1‑140 (MQ=255)                                                                                                                       tgAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTcc     >  2:1051/1‑140 (MQ=255)                                                                                                                       tgAACCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGGTTTTCATGCGGAAAAACAGCGCAAATATCAAAGAAACAGACTGGGTATTACTCTcc     >  2:916510/1‑140 (MQ=255)                                                                                                                          aCCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGa  <  1:136436/140‑1 (MQ=255)                                                                                                                          aCCGATGGTCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGa  <  1:763886/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    AGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGA  >  NC_000913/1144315‑1144575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑