Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,187,155	C→T	A362A (GCG→GCA)	roxA ←	ribosomal protein‑arginine oxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,187,155	0	C	T	100.0%	132.5 / NA	38	A362A (GCG→GCA)	roxA	ribosomal protein‑arginine oxygenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/16);  total (22/16)

TGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCATACACG  >  NC_000913/1187024‑1187290                                                                                                                                    |                                                                                                                                        tGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAg                                                                                                                                 <  1:479183/140‑1 (MQ=255) tGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAg                                                                                                                                 >  1:577167/1‑140 (MQ=255)       tGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATcgc                                                                                                                           <  1:940577/140‑1 (MQ=255)        gATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATcgcg                                                                                                                          <  2:577167/140‑1 (MQ=255)                gTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGa                                                                                                                  >  1:866720/1‑140 (MQ=255)                                 gcgcAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCAt                                                                                                 <  1:763609/140‑1 (MQ=255)                                   gcAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCg                                                                                               >  1:963857/1‑140 (MQ=255)                                                    aaaGGGATGCGGCATGTGATGCCGCATCCGGCCTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGt                                                                              >  2:438390/1‑140 (MQ=255)                                                      aGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCa                                                                            >  2:236844/1‑140 (MQ=255)                                                           tGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCa                                                                       >  1:224517/1‑140 (MQ=255)                                                                     gATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg                                                             <  1:867052/140‑1 (MQ=255)                                                                      aTGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg                                                             >  2:536005/1‑139 (MQ=255)                                                                      aTGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg                                                             <  1:536005/139‑1 (MQ=255)                                                                          cGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGt                                                        >  2:187078/1‑140 (MQ=255)                                                                            cATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGc                                                      >  1:187360/1‑140 (MQ=255)                                                                              tCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg                                                             <  1:419818/131‑1 (MQ=255)                                                                              tCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg                                                             >  2:419818/1‑131 (MQ=255)                                                                              tCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCg                                                           <  1:824767/133‑1 (MQ=255)                                                                              tCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCg                                                           >  2:824767/1‑133 (MQ=255)                                                                                cGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCg                                                           >  1:259013/1‑131 (MQ=255)                                                                                cGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCg                                                           <  2:259013/131‑1 (MQ=255)                                                                                             aaCTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGt                                     >  2:257845/1‑140 (MQ=255)                                                                                                       tCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATc                           <  1:383008/140‑1 (MQ=255)                                                                                                       tCGAAGAACCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATc                           >  2:383008/1‑140 (MQ=255)                                                                                                         gaagaaCCAATGCCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGa                         <  2:62121/140‑1 (MQ=255)                                                                                                          aagaaCCAATACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGAt                        <  1:257845/140‑1 (MQ=255)                                                                                                                   tACCCGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCAcc               <  1:187078/140‑1 (MQ=255)                                                                                                                     cccGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCAt             <  1:109674/140‑1 (MQ=255)                                                                                                                       cGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCa              >  1:808005/1‑137 (MQ=255)                                                                                                                       cGCTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCa              <  2:808005/137‑1 (MQ=255)                                                                                                                         cTATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGc         >  2:222382/1‑140 (MQ=255)                                                                                                                          tATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGAt                        <  1:910396/124‑1 (MQ=255)                                                                                                                          tATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCa        >  1:559559/1‑140 (MQ=255)                                                                                                                          tATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCa        >  1:431601/1‑140 (MQ=255)                                                                                                                          tATTGACCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTATCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGAt                        >  2:910396/1‑124 (MQ=255)                                                                                                                               aCCAGTGCCGCGAGCATCGCGAGGAGTGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCATacac   >  2:831303/1‑140 (MQ=255)                                                                                                                               aCCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGAtcttct                   <  1:885646/124‑1 (MQ=255)                                                                                                                               aCCAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGAtcttct                   >  2:885646/1‑124 (MQ=255)                                                                                                                                ccAGTGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCATACAcg  >  2:212812/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        TGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCATACACG  >  NC_000913/1187024‑1187290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑