Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,227,874	A→G	N65S (AAT→AGT)	ycgL →	PF05166 family protein YcgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,227,874	0	A	G	100.0%	175.3 / NA	50	N65S (AAT→AGT)	ycgL	PF05166 family protein YcgL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/23);  total (27/23)

GACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCG  >  NC_000913/1227747‑1228010                                                                                                                                |                                                                                                                                         gACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGa                                                                                                                              <  2:412433/140‑1 (MQ=255) gACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGa                                                                                                                              <  1:282570/140‑1 (MQ=255)  aCCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGaa                                                                                                                             >  1:1013735/1‑140 (MQ=255)           aTTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTaaa                                                                                                                    <  2:1013735/140‑1 (MQ=255)            tttATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAAc                                                                                                                   >  1:386906/1‑140 (MQ=255)             ttATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACa                                                                                                                  >  1:764507/1‑140 (MQ=255)                 atGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGATGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCa                                                                                                              >  2:921286/1‑140 (MQ=255)                 atGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCa                                                                                                              >  2:138220/1‑140 (MQ=255)                 atGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCa                                                                                                              >  2:430900/1‑140 (MQ=255)                 atGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCa                                                                                                              >  1:549593/1‑140 (MQ=255)                   gTCGAAAAAAAAGACGGTTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCAtt                                                                                                            >  2:783954/1‑140 (MQ=255)                   gTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTaaa                                                                                                                    >  1:412281/1‑132 (MQ=255)                   gTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTaaa                                                                                                                    <  2:412281/132‑1 (MQ=255)                   gTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCAtt                                                                                                            >  1:431999/1‑140 (MQ=255)                    tCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTa                                                                                                           >  2:131111/1‑140 (MQ=255)                     cGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTaa                                                                                                          >  2:78939/1‑140 (MQ=255)                     cGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTaa                                                                                                          >  2:425683/1‑140 (MQ=255)                          aaaaaGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAg                                                                                                     <  2:386906/140‑1 (MQ=255)                           aaaaGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGc                                                                                                    <  2:431999/140‑1 (MQ=255)                            aaaGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCa                                                                                                   <  1:78939/140‑1 (MQ=255)                                       ttCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTAtt                                                                                        >  1:242080/1‑140 (MQ=255)                                                     aGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGcca                                                                          <  1:138220/140‑1 (MQ=255)                                                          cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGccac                                                                         >  2:260999/1‑136 (MQ=255)                                                          cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGccac                                                                         <  1:260999/136‑1 (MQ=255)                                                          cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCaccacc                                                                     >  2:132490/1‑140 (MQ=255)                                                          cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCaccacc                                                                     >  1:592911/1‑140 (MQ=255)                                                            gatgaAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCg                                                                   <  1:430900/140‑1 (MQ=255)                                                                     ttttGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGc                                                          <  2:265974/140‑1 (MQ=255)                                                                      tttGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCt                                                         <  1:425683/140‑1 (MQ=255)                                                                             aGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCaa                                                  <  1:131111/140‑1 (MQ=255)                                                                               ccTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACa                                                <  2:764507/140‑1 (MQ=255)                                                                                         cGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTc                                      >  1:226590/1‑140 (MQ=255)                                                                                          gatgatTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCa                                     <  2:242080/140‑1 (MQ=255)                                                                                          gatgatTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCa                                     <  1:519118/140‑1 (MQ=255)                                                                                             gatTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACa                                                >  1:725253/1‑126 (MQ=255)                                                                                             gatTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACa                                                <  2:725253/126‑1 (MQ=255)                                                                                             gatTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATgg                                  >  1:782542/1‑140 (MQ=255)                                                                                                      gCTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGaaaa                         <  1:694239/140‑1 (MQ=255)                                                                                                       cTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCa                                     <  1:472366/127‑1 (MQ=255)                                                                                                       cTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCa                                     >  2:472366/1‑127 (MQ=255)                                                                                                       cTGGATGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAAc                        >  2:191732/1‑140 (MQ=255)                                                                                                           aTGGGCGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAgac                    <  1:272914/140‑1 (MQ=255)                                                                                                                cGGAAGAAACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGacac               <  1:783954/140‑1 (MQ=255)                                                                                                                  gaagaaACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTa             >  1:778521/1‑140 (MQ=255)                                                                                                                  gaagaaACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTa             <  2:778521/140‑1 (MQ=255)                                                                                                                    agaaACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTa             <  1:103629/138‑1 (MQ=255)                                                                                                                    agaaACTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTa             >  2:103629/1‑138 (MQ=255)                                                                                                                         cTGGTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATa      >  2:267239/1‑140 (MQ=255)                                                                                                                            gTCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAAcc   >  2:136822/1‑140 (MQ=255)                                                                                                                             tCAGTGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCg  >  2:548991/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                         GACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCG  >  NC_000913/1227747‑1228010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑